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AB175472

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568)

5

(1 Review)

|

(58 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 58 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175472)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175472)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175472 Alexa Fluor® 568 donkey anti-mouse IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175472)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175472)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

Flow Cyt, ELISA, IHC-Fr, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (58)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:159 PubMed40528237

2025

Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoqiang Guan,Linglin Zhang,Kristian Nzogang Fomo,Jie Yang,Norbert Pfeiffer,Franz H Grus

eLife 13: PubMed40476343

2025

Clearance of protein aggregates during cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Shoukang Du,Yuhan Wang,Bowen Chen,Shuangshuang Xie,Kuan Yoow Chan,David C Hay,Ting Gang Chew

Acta physiologica (Oxford, England) 241:e70060 PubMed40387445

2025

Chronic High-Fat Diet Consumption Followed by Lipopolysaccharide Challenge Induces Persistent and Long-Lasting Microglial Priming, Mediates Synaptic Elimination via Complement C1q, and Leads to Behavioral Abnormalities in Male Wistar Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Titikorn Chunchai,Hiranya Pintana,Chanon Kunasol,Patcharapong Pantiya,Busarin Arunsak,Sasiwan Kerdphoo,Wichwara Nawara,Suriphan Donchada,Nattayaporn Apaijai,Jirapas Sripetchwandee,Chanisa Thonusin,Nipon Chattipakorn,Siriporn C Chattipakorn

mBio 16:e0098425 PubMed40298449

2025

Role of glucuronoxylomannan and steryl glucosides in protecting against cryptococcosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Soares Matos,Samyr M Querobino,Veronica S Brauer,Luna S Joffe,Nivea Pereira de Sa,Caroline Mota Fernandes,Deveney DaSilva,Vanessa A da Silva,Marinaldo Pacífico Cavalcanti Neto,Tyler Normile,Hengwei Zhu,Surita R Bhatia,Li Tan,Parastoo Azadi,Christian Heiss,Tamara L Doering,Maurizio Del Poeta

Cell 188:3329-3348.e31 PubMed40250419

2025

Fate and state transitions during human blood vessel organoid development.

Applications

Unspecified application

Species

Unspecified reactive species

Marina T Nikolova,Zhisong He,Makiko Seimiya,Gustav Jonsson,Wuji Cao,Ryo Okuda,Reiner A Wimmer,Ryoko Okamoto,Josef M Penninger,J Gray Camp,Barbara Treutlein

Frontiers in cellular neuroscience 19:1524470 PubMed40177584

2025

Chemogenetic activation of Gq signaling modulates dendritic development of cortical neurons in a time- and layer-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ina Köhler,Lisa Marie Rennau,Adriana Rehm,Julia Große,Steffen Gonda,Andrea Räk,Christian Riedel,Petra Wahle

PloS one 20:e0317878 PubMed40106424

2025

Tolerance of Placozoa for temperate climates: Evidence for known and new placozoan clades in the southern waters of Australia.

Applications

Unspecified application

Species

Unspecified reactive species

Bree A Wright,Hans-Jürgen Osigus,Moritz J Schmidt,Julian Ratcliffe,Kai Kamm,Gabriela C Martinez-Ortiz,Martina Rehn,Marc Kvansakul,Bernd Schierwater,Patrick O Humbert

Journal of translational medicine 23:189 PubMed39956898

2025

MicroRNA-27a transfected dental pulp stem cells undergo odonto/osteogenic differentiation via targeting DKK3 and SOSTDC1 in Wnt/BMP signaling in vitro and enhance bone formation in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ziniu Yu,Nobuyuki Kawashima,Keisuke Sunada-Nara,Shihan Wang,Peifeng Han,Thoai Quoc Kieu,Chunmei Ren,Sonoko Noda,Kento Tazawa,Takashi Okiji

Islets 16:2436696 PubMed39641365

2024

Changes in immunofluorescence staining during islet regeneration in a cystic fibrosis-related diabetes (CFRD) ferret model.

Applications

Unspecified application

Species

Unspecified reactive species

Sawash M Mohammed,Robert N Bone,Jacqueline Del Carmen Aquino,Raghavendra G Mirmira,Carmella Evans-Molina,Heba M Ismail

Frontiers in physiology 15:1425620 PubMed39318362

2024

Endothelial cell elongation and alignment in response to shear stress requires acetylation of microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Katiannah Moise,Keerthana M Arun,Maalavika Pillai,Jocelynda Salvador,Aarya S Mehta,Yogesh Goyal,M Luisa Iruela-Arispe
View all publications

Product promise

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