Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568)
5
(1 Review)
|
(58 Publications)
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 58 publications.
View Alternative Names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175472)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175472 Alexa Fluor® 568 donkey anti-mouse IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175472)
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Publications (58)
Recent publications for all applications. Explore the full list and refine your search
Journal of neuroinflammation 22:159 PubMed40528237
2025
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eLife 13: PubMed40476343
2025
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Acta physiologica (Oxford, England) 241:e70060 PubMed40387445
2025
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mBio 16:e0098425 PubMed40298449
2025
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Cell 188:3329-3348.e31 PubMed40250419
2025
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Frontiers in cellular neuroscience 19:1524470 PubMed40177584
2025
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PloS one 20:e0317878 PubMed40106424
2025
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Journal of translational medicine 23:189 PubMed39956898
2025
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Islets 16:2436696 PubMed39641365
2024
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Frontiers in physiology 15:1425620 PubMed39318362
2024
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