AB175700

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

Name: Donkey Anti Mouse (IgG) secondary antibody preadsorbed Alexa Fluor® 568
Brand: Abcam Limited
SKU: AB175700
Price: 270 USD
Availability: InStock

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(39 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 39 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

3 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175700 Alexa Fluor® 568 Donkey anti-Mouse IgG (H&L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175700 Alexa Fluor® 568 Donkey anti-Mouse IgG (H&L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

Emmission spectra of the Alexa Fluor^® conjugated secondary antibodies.

615 Texas Red®

Donkey Anti-Mouse IgG H&L (Texas Red ®)
Unconjugated

Donkey Anti-Mouse IgG H&L preadsorbed
10nm Gold

Donkey Anti-Mouse IgG H&L (10nm Gold)
572 TRITC

Donkey Anti-Mouse IgG H&L (TRITC)
Alkaline Phosphatase

Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
578 PE

Donkey Anti-Mouse IgG H&L (PE) preadsorbed
576 DyLight® 550

Donkey Anti-Mouse IgG H&L (DyLight® 550)
618 DyLight® 594

Donkey Anti-Mouse IgG H&L (DyLight® 594)
519 Alexa Fluor® 488

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)
665 Alexa Fluor® 647

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
617 Alexa Fluor® 594

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)
565 Alexa Fluor® 555

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed
805 Alexa Fluor® 790

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed
775 Alexa Fluor® 750

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750)
421 Alexa Fluor® 405

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)
Biotin

Donkey Anti-Mouse IgG H&L (Biotin)
518 DyLight® 488

Donkey Anti-Mouse IgG H&L (DyLight® 488)
519 FITC

Donkey Anti-Mouse IgG H&L (FITC)
HRP

Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
673 DyLight® 650

Donkey Anti-Mouse IgG H&L (DyLight® 650)
702 Alexa Fluor® 680

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

ELISA, Flow Cyt, IHC-P, IHC-Fr, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 26:179 PubMed40770605

2025

Compromised PGF2α signaling in the paraventricular hypothalamic nucleus contributes to the central sensitization of the nitroglycerin-induced chronic migraine in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xianrong Hu,Lunquan Wu,Li Wang,Congxue Peng,Yunyun Tian,Qian Zhu,Hongwei Zhu,Liang Nie,Li Yin,Yuehui Zhang,Jiang Bian

Frontiers in immunology 16:1592597 PubMed40735328

2025

Revealing the impact of quorum sensing molecule 2'-aminoacetophenone on the human bronchial-airway epithelium and pulmonary endothelium using a human airway-on-a-chip.

Applications

Unspecified application

Species

Unspecified reactive species

Shifu Aggarwal,Arijit Chakraborty,Vijay K Singh,Stephen Lory,Katia Karalis,Laurence G Rahme

Journal of the National Cancer Center 5:221-235 PubMed40265092

2025

The single-cell immune landscape of HIV-associated aggressive B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Zhang,Zailin Yang,Xiaoqing Xie,Jun Li,Qing Xiao,Guofa Xu,Ben Ma,Xudong Xie,Yi Liu,Liuyue Zhai,Yifeng Tang,Huihui Fu,Sanxiu He,Tingting Liu,Dehong Huang,Censi Zeng,Yixing Zhou,Renzhi Hu,Binling Guo,Chaoyu Wang,Shunsi Liang,Qin Luo,Jing Lv,Yingyu Nan,Jieping Li,Qiying Li,Shengqiang Wang,Yongzhong Wu,Yao Liu

Immunity & ageing : I & A 22:9 PubMed39994686

2025

Secreted IgM deficiency alters the retinal landscape enhancing neurodegeneration associated with aging.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Webster,Sydney M Les,Nico Deleon,Daken M Heck,Naomi L Tsuj,Michael J Clemente,Prentiss Jones,Nichol E Holodick

Fluids and barriers of the CNS 22:4 PubMed39789614

2025

Plasma S100β is a predictor for pathology and cognitive decline in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Geetika Nehra,Bryan J Maloney,Rebecca R Smith,Wijitra Chumboatong,Erin L Abner,Peter T Nelson,Björn Bauer,Anika M S Hartz

Neural regeneration research 20:2068-2083 PubMed39254567

2024

FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yao,Yuan Li,Xi Liu,Wenping Liang,Yu Li,Liyong Wu,Zhe Wang,Weihong Song

The Journal of clinical endocrinology and metabolism 110:715-729 PubMed39172542

2024

GIP Receptor Antagonism Eliminates Paradoxical Growth Hormone Secretion in Some Patients With Acromegaly.

Applications

Unspecified application

Species

Unspecified reactive species

Mette H Jensen,Lærke S Gasbjerg,Kirsa Skov-Jeppesen,Jens C B Jacobsen,Steen S Poulsen,Cuiqi Zhou,Ruta Jakubauskaite,Frantz R Poulsen,Christian Bonde,Mahmoud Albarazi,Bo Halle,Charlotte B Christiansen,Samra J Sanni,Sarah Byberg,Bjørn Hoe,Jens J Holst,Flemming Dela,Aase K Rasmussen,Filip K Knop,Mai C Arlien-Søborg,Shlomo Melmed,Jens Otto L Jørgensen,Marianne S Andersen,Bolette Hartmann,Marianne C Klose,Ulla Feldt-Rasmussen,Alexander H Sparre-Ulrich,Mette M Rosenkilde

Journal of neural engineering 21: PubMed38788704

2024

Neuronal functional connectivity is impaired in a layer dependent manner near chronically implanted intracortical microelectrodes in C57BL6 wildtype mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Chen,Adam M Forrest,Guillermo Gonzalez Burgos,Takashi D Y Kozai

Biomaterials 302:122316 PubMed37738741

2023

Cell-specific alterations in autophagy-lysosomal activity near the chronically implanted microelectrodes.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Chen,Camila Garcia Padilla,Kirill Kiselyov,Takashi D Y Kozai

Biomaterials 301:122210 PubMed37413842

2023

Pro-myelinating clemastine administration improves recording performance of chronically implanted microelectrodes and nearby neuronal health.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Chen,Franca Cambi,Takashi D Y Kozai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

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Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (39)

Product promise