AB150108

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)

Name: Donkey Anti-Mouse IgG (Alexa Fluor® 594) secondary antibody (ab150108) | Abcam
Brand: Abcam Limited
SKU: AB150108
Price: 130 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(167 Publications)

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) is a secondary antibody with a maximum absorption wavelength of 590nm and a maximum emission wavelength of 617nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 120 publications

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

3 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

ICC/IF

AbReview41355****

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

ab16287 staining SSEA4 in rat stem cells derived from tendon by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with parafolmaldehyde, permeabilized with PBS containing 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hour at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG H&L (ab150108) (1/1000) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Sun Jeong Kim.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 2μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150108 Alexa Fluor® 594 donkey anti-mouse IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

Emmission spectra of the Alexa Fluor^® conjugated secondary antibodies.

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

Flow Cyt, ICC/IF, IHC-P, ELISA, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<a href='/en-us/products/primary-antibodies/alexa-fluor-594-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab178000'>ab178000</a> - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control to complement this secondary antibody." } } }

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Product details

This antibody reacts with the heavy and light chains of Mouse IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (167)

Recent publications for all applications. Explore the full list and refine your search

Biology methods & protocols 10:bpaf068 PubMed41018846

2025

A robust protocol for proteomic profiling of secreted proteins in conditioned culture medium.

Applications

Unspecified application

Species

Unspecified reactive species

Takayoshi Otsuka,Atsushi Hatano,Masaki Matsumoto,Hideaki Matsui

International journal of molecular sciences 26: PubMed40869287

2025

Differential Sensitivity of Hippocampal GABAergic Neurons to Hypoxia and Ischemia-like Conditions Correlates with the Type of Calcium-Binding Protein Expressed.

Applications

Unspecified application

Species

Unspecified reactive species

Maria V Turovskaya,Valery P Zinchenko

Nature communications 16:7350 PubMed40783491

2025

UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ambre Bender,Marion Morel,Michael Dumas,Muriel Klopfenstein,Naël Osmani,Maxim V C Greenberg,Déborah Bourc'his,Norbert B Ghyselinck,Michael Weber

Science advances 11:eadu4270 PubMed40749060

2025

Neuronal Reg3β/macrophage TNF-α-mediated positive feedback signaling contributes to pain chronicity in a rat model of CRPS-I.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Nie,Boyu Liu,Chengyu Yin,Zishan Dong,Yushuang Pan,Peiyi Li,Qimiao Hu,Jie Wang,Yan Tai,Xiaomei Shao,Guihua Tian,Chuan Wang,Jianqiao Fang,Boyi Liu

Cellular and molecular neurobiology 45:47 PubMed40394428

2025

Atorvastatin Protects Against the Macrophage/Microglia-Related Neuroinflammation via Inhibiting Lipocalin-2 in Mouse Experimental Intracerebral Hemorrhage Model.

Applications

Unspecified application

Species

Unspecified reactive species

Guangming Wang,Hongkang Hu,Junbin Liu,Xiaowei Fei,Yanan Dou,Li Wang,Lin Ying,Guohan Hu,Danfeng Zhang,Lei Jiang,Jialiang Wei

PloS one 20:e0323633 PubMed40373038

2025

Klotho null mutation leads to retinal degeneration characterized by functional impairment, gliosis, and deposition of amyloid-beta and hyperphosphorylated tau proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Jia Chen,Chun-Yen Wu,Fang-Yan Hsiao,Jing-Chun Ma,Gabriel Lai,Han-Hsin Chang,David Pei-Cheng Lin

Alzheimer's research & therapy 17:95 PubMed40301979

2025

The autocrine motility factor receptor delays the pathological progression of Alzheimer's disease via regulating the ubiquitination-mediated degradation of APP.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zhang,Congcong Liu,Jing Liu,Yuting Cui,Yuli Hou,Qiao Song,Xiaomin Zhang,Xiaoling Wang,Qian Zhang,Min Cao,Wenchao Wang,Peichang Wang,Yaqi Wang

Nature biotechnology : PubMed40269321

2025

Treatment of acute myeloid leukemia models by targeting a cell surface RNA-binding protein.

Applications

Unspecified application

Species

Unspecified reactive species

Benson M George,Maria Eleftheriou,Eliza Yankova,Jonathan Perr,Peiyuan Chai,Gianluca Nestola,Karim Almahayni,Siân Evans,Aristi Damaskou,Helena Hemberger,Charlotta G Lebedenko,Justyna Rak,Qi Yu,Ece Bapcum,James Russell,Jaana Bagri,Regan F Volk,Malte Spiekermann,Richard M Stone,George Giotopoulos,Brian J P Huntly,Joanna Baxter,Fernando Camargo,Jie Liu,Balyn W Zaro,George S Vassiliou,Leonhard Möckl,Jorge de la Rosa,Ryan A Flynn,Konstantinos Tzelepis

Bioengineering (Basel, Switzerland) 12: PubMed40150724

2025

Six-Month Patency of Long Carotid Bypass Grafts Constructed with In-Body Tissue Architecture-Induced Small-Diameter Biotubes in a Goat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuki Mori,Tadashi Umeno,Takayuki Kawashima,Takashi Shuto,Ryosuke Iwai,Lupeng Teng,Tsutomu Tajikawa,Yasuhide Nakayama,Shinji Miyamoto

Cells 14: PubMed40136713

2025

Extracellular Vesicles from Regenerating Skeletal Muscle Mitigate Muscle Atrophy in an Amyotrophic Lateral Sclerosis Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Gao,Aria Sikal,Rachel Hankin,Yaochao Zheng,Elijah Sterling,Kenny Chan,Yao Yao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (167)

Product promise