Donkey anti-mouse IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 120 publications
Images
Publications
Filter by
- PNAS nexus 3:pgad4822024Suppression of neuropathic pain in the circadian clock-deficient mice involves up-regulation of endocannabinoid system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWakaba Yamakawa,Sai Yasukochi,Yuya Tsurudome,Naoki Kusunose,Yuta Yamaguchi,Akito Tsuruta,Naoya Matsunaga,Kentaro Ushijima,Satoru Koyanagi,Shigehiro OhdoPubMed 38239754
- Scientific reports 13:200102023Efficient protocol for the differentiation of kidney podocytes from induced pluripotent stem cells, involving the inhibition of mTOR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Yasuda,Tadashi Kato,Mai Okano,Hiromi Yamashita,Yoshikazu Matsuoka,Yasumasa Shirouzu,Tatsuya Fujioka,Fumiyuki Hattori,Shoji Tsuji,Kazunari Kaneko,Hirofumi HitomiPubMed 37973990
- Nature neuroscience 26:2104-21212023The APOE-R136S mutation protects against APOE4-driven Tau pathology, neurodegeneration and neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaxine R Nelson,Peng Liu,Ayushi Agrawal,Oscar Yip,Jessica Blumenfeld,Michela Traglia,Min Joo Kim,Nicole Koutsodendris,Antara Rao,Brian Grone,Yanxia Hao,Seo Yeon Yoon,Qin Xu,Samuel De Leon,Tenzing Choenyi,Reuben Thomas,Francisco Lopera,Yakeel T Quiroz,Joseph F Arboleda-Velasquez,Eric M Reiman,Robert W Mahley,Yadong HuangPubMed 37957317
- Frontiers in immunology 14:12806682023Optimal temperature for the long-term culture of adult porcine islets for xenotransplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoaki Sakata,Gumpei Yoshimatsu,Ryo Kawakami,Chikao Aoyagi,Shohta KodamaPubMed 37901206
- Immunology 171:104-1162023Unlocking the role of the B7-H4 polymorphism in psoriasis: Insights into methotrexate treatment outcomes: A prospective cohort study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Wang,Zhicheng Wang,Wenjing Yang,Ling Han,Qiong Huang,Nikhil Yawalkar,Zhenghua Zhang,Yu Yao,Kexiang YanPubMed 37814391
- mBio 14:e00934232023PKR-mediated stress response enhances dengue and Zika virus replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaissa Ricciardi-Jorge,Edroaldo Lummertz da Rocha,Edgar Gonzalez-Kozlova,Gabriela Flavia Rodrigues-Luiz,Brian J Ferguson,Trevor Sweeney,Nerea Irigoyen,Daniel Santos MansurPubMed 37732809
- Frontiers in microbiology 14:12016402023Validation of flavivirus infectious clones carrying fluorescent markers for antiviral drug screening and replication studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiubov Cherkashchenko,Nathalie Gros,Alice Trausch,Aymeric Neyret,Mathilde Hénaut,Gregor Dubois,Matthieu Villeneuve,Christine Chable-Bessia,Sébastien Lyonnais,Andres Merits,Delphine MuriauxPubMed 37779700
- Frontiers in molecular neuroscience 16:10278982023Synaptic expression of TAR-DNA-binding protein 43 in the mouse spinal cord determined using super-resolution microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew J Broadhead,Ani Ayvazian-Hancock,Katherine Doucet,Owen Kantelberg,Lesley Motherwell,Fei Zhu,Seth G N Grant,Mathew H Horrocks,Gareth B MilesPubMed 37671010
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23032912023Lepr-Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanting Zhang,Weimin Lin,Shuang Jiang,Peng Deng,Linfeng Liu,Qian Wang,Rui Sheng,Hui Sophie Shu,Lijun Wang,Weiguo Zou,Bo O Zhou,Junjun Jing,Ling Ye,Bo Yu,Shiwen Zhang,Quan YuanPubMed 37553778
- Advanced healthcare materials :e23015522023Microfluidic Generation of Thin-Shelled Polyethylene Glycol-Tyramine Microgels for Non-Invasive Delivery of Immunoprotected β-Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuno Araújo-Gomes,Barbara Zoetebier-Liszka,Bas van Loo,Malin Becker,Suzanne Nijhuis,Alexandra M Smink,Bart J de Haan,Paul de Vos,Marcel Karperien,Jeroen LeijtenPubMed 37548084
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000.00000 - 1/4000.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Donkey anti-mouse IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 120 publications
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
Overlay histogram showing Jurkat cells stained with Anti-CD3 antibody [MEM-57] ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 antibody [MEM-57] ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Donkey anti-mouse IgG H&L (Alexa Fluor® 647) (ab150107) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (red) was ab150107 Alexa Fluor® 647 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
Emmission spectra of the Alexa Fluor® conjugatedsecondary antibodies.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com