Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications
Images
Publications
Filter by
- Cell 186:5910-5924.e172023A tridimensional atlas of the developing human head.Applications:IHC - WmtReactive species:HumanRaphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain ChédotalPubMed 38070509
- iScience 26:1069852023Dissection of insular cortex layer 5 reveals two sublayers with opposing modulatory roles in appetitive drinking behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Takemoto,Shigeki Kato,Kazuto Kobayashi,Wen-Jie SongPubMed 37378339
- Journal of neuroinflammation 20:1092023CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal horn.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Wang,Chengyu Yin,Yushuang Pan,Yunqin Yang,Wei Li,Huadong Ni,Boyu Liu,Huimin Nie,Ruoyao Xu,Huina Wei,Yunwen Zhang,Yuanyuan Li,Qimiao Hu,Yan Tai,Xiaomei Shao,Jianqiao Fang,Boyi LiuPubMed 37158939
- Nature food 4:35-502023Spontaneous immortalization of chicken fibroblasts generates stable, high-yield cell lines for serum-free production of cultured meat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL Pasitka,M Cohen,A Ehrlich,B Gildor,E Reuveni,M Ayyash,G Wissotsky,A Herscovici,R Kaminker,A Niv,R Bitcover,O Dadia,A Rudik,A Voloschin,M Shimoni,Y Cinnamon,Y NahmiasPubMed 37118574
- Neural regeneration research 18:2011-20182023Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing-Qiang He,Ai-Cheng Li,Yu-Xuan Hou,Hui Li,Xing-Yuan Zhang,Hui-Fei Hao,Hong-Hua Song,Ri-Xin Cai,Ying-Jie Wang,Yue Zhou,Yong-Jun WangPubMed 36926727
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e228132023O-glycan structures in apo(a) subunit of human lipoprotein(a) suppresses the pro-angiogenic activity of galectin-1 on human umbilical vein endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVasantha Kalaivani,Mahesh S Krishna,Asokan Aneesh Kumar,Gopika Satheesh,Abdul JaleelPubMed 36809652
- iScience 26:1058852023Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Jin,Xiangfeng Chen,Hanyu Zheng,Wanxiong Cai,Xurong Lin,Xiangxing Kong,Yingchun Ni,Jingjia Ye,Xiaodan Li,Luoan Shen,Binjie Guo,Zeinab Abdelrahman,Songlin Zhou,Susu Mao,Yaxian Wang,Chun Yao,Xiaosong Gu,Bin Yu,Zhiping Wang,Xuhua WangPubMed 36654858
- Addiction biology 27:e132382022Embryonic ethanol exposure induces ectopic Hcrt and MCH neurons outside hypothalamus in rats and zebrafish: Role in ethanol-induced behavioural disturbances.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam D Collier,Nushrat Yasmin,Guo-Qing Chang,Olga Karatayev,Nailya Khalizova,Milisia Fam,Abdul R Abdulai,Boyi Yu,Sarah F LeibowitzPubMed 36301208
- Nature communications 13:63772022Eya-controlled affinity between cell lineages drives tissue self-organization during Drosophila oogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVanessa Weichselberger,Patrick Dondl,Anne-Kathrin ClassenPubMed 36289235
- iScience 25:1051722022Fast generation of forebrain oligodendrocyte spheroids from human embryonic stem cells by transcription factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Ma,Yuting Mei,Peibo Xu,Yan Cheng,Zhiwen You,Xiaoli Ji,Deyi Zhuang,Wenhao Zhou,Yuejun Chen,Man XiongPubMed 36217550
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes ab176103 - Mouse monoclonal IgG1 (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody. |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Mouse IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150111)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 10μg/ml) overnight at +4°C. The secondary antibody (red) was ab150111 Alexa Fluor® 647 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150111)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com