AB175774

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680)

Name: Donkey Anti Mouse (IgG) secondary antibody Alexa Fluor® 680 (ab175774) | Abcam
Brand: Abcam Limited
SKU: AB175774
Price: 145 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(19 Publications)

Suitable for WB. Cited in 19 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images

Lab

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680) (AB175774)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175774 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 5 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 2 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 1 µg

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680) (ab175774) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 52 kDa

false

615 Texas Red®

Donkey Anti-Mouse IgG H&L (Texas Red ®)
Unconjugated

Donkey Anti-Mouse IgG H&L preadsorbed
10nm Gold

Donkey Anti-Mouse IgG H&L (10nm Gold)
572 TRITC

Donkey Anti-Mouse IgG H&L (TRITC)
Alkaline Phosphatase

Donkey Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
578 PE

Donkey Anti-Mouse IgG H&L (PE) preadsorbed
576 DyLight® 550

Donkey Anti-Mouse IgG H&L (DyLight® 550)
618 DyLight® 594

Donkey Anti-Mouse IgG H&L (DyLight® 594)
519 Alexa Fluor® 488

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)
665 Alexa Fluor® 647

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
617 Alexa Fluor® 594

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)
565 Alexa Fluor® 555

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed
805 Alexa Fluor® 790

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed
775 Alexa Fluor® 750

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750)
421 Alexa Fluor® 405

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)
603 Alexa Fluor® 568

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed
Biotin

Donkey Anti-Mouse IgG H&L (Biotin)
518 DyLight® 488

Donkey Anti-Mouse IgG H&L (DyLight® 488)
519 FITC

Donkey Anti-Mouse IgG H&L (FITC)
HRP

Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
673 DyLight® 650

Donkey Anti-Mouse IgG H&L (DyLight® 650)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Applications

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

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Product details

We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor^® 680), ab175774 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Mouse IgG H&L (Alexa Fluor^® 680), ab175774 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:6 PubMed39844330

2025

Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyuan Li,Hang Yuan,Xiaofei Yang,Yingfeng Lei,Jianqi Lian

Experimental & molecular medicine 55:2248-2259 PubMed37779148

2023

Vascular regeneration and skeletal muscle repair induced by long-term exposure to SDF-1α derived from engineered mesenchymal stem cells after hindlimb ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Ju Kim,Jae-Hyun Park,Hyeok Kim,Woo-Sup Sim,Seokbeom Hong,Yeon-Jik Choi,Hyo-Jin Kim,Soon Min Lee,Dongha Kim,Sun-Woong Kang,Kiwon Ban,Hun-Jun Park

Redox biology 66:102874 PubMed37683300

2023

Adverse bioenergetic effects of N-acyl amino acids in human adipocytes overshadow beneficial mitochondrial uncoupling.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Herrnhold,Isabel Hamp,Oliver Plettenburg,Martin Jastroch,Michaela Keuper

Cellular signalling 106:110640 PubMed36841274

2023

β3 adrenergic receptor activation modulates connexin 43 activity to relax human myometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Hazik Asif,Scott D Barnett,Dylan Saxon,Hazar Younis,Iain L O Buxton

Frontiers in endocrinology 13:860261 PubMed36187124

2022

S100P promotes trophoblast syncytialization during early placenta development by regulating YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Hanjing Zhou,Yibin Pan,Weijie Yang,Chenqiong Zhao,Xiaohe Sun,Binbin Hong,Xiaoying Jin,Tai Zhang,Yinli Zhang,Na Liu,Songying Zhang,Haiyan Zhu

Reproductive sciences (Thousand Oaks, Calif.) 30:124-134 PubMed35380411

2022

Title: β3 Adrenergic Receptor Signaling in the Human Myometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Hazik Asif,Scott D Barnett,Iain L O Buxton

Cancer genetics 264-265:40-49 PubMed35354106

2022

Functional analysis of ATM variants in a high risk cohort provides insight into missing heritability.

Applications

Unspecified application

Species

Unspecified reactive species

Scott L Baughan,Fatima Darwiche,Michael A Tainsky

Cell death discovery 7:62 PubMed33782397

2021

Germline mutations in apoptosis pathway genes in ovarian cancer; the functional role of a TP53I3 (PIG3) variant in ROS production and DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia R Chaudhry,Jaime Lopes,Nancy K Levin,Hasini Kalpage,Michael A Tainsky

Oxidative medicine and cellular longevity 2021:6678276 PubMed33859781

2021

Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Di Zhang,Qi Shen,Xiaolei Wu,Da Xing

Science advances 7: PubMed33563593

2021

Architecture and regulation of an enterobacterial cellulose secretion system.

Applications

Unspecified application

Species

Unspecified reactive species

Wiem Abidi,Samira Zouhir,Meryem Caleechurn,Stéphane Roche,Petya Violinova Krasteva

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680)

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680) (AB175774)

Related conjugates and formulations (21)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (19)

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