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AB175738

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750)

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(14 Publications)

Suitable for WB. Cited in 14 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images
Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) (AB175738)
  • WB

Lab

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) (AB175738)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175738 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) (ab175738) at 1/10000 dilution

Predicted band size: 33 kDa,36 kDa,42 kDa,47 kDa,61 kDa,80 kDa

Observed band size: 100 kDa,50 kDa,60 kDa,74 kDa

false

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB

applications

Clonality

Polyclonal

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Alternative Products

Secondary Antibodies

AB150105

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150105)

  • 4
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Secondary Antibodies

AB150106

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)

  • 5
  • 1
Secondary Antibodies

AB150107

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)

Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB150107)

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  • 2
Secondary Antibodies

AB150108

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

  • 5
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Secondary Antibodies

AB175658

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

  • 5
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Secondary Antibodies

AB175700

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

  • 0
  • 0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }
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Product details

We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750), ab175738 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingDonkey Anti-Mouse IgG H&L (Alexa Fluor® 750), ab175738 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8500 PubMed41006220

2025

VTA monosynaptic connections by local glutamate and GABA neurons and their distinct roles in behavior.

Applications

Unspecified application

Species

Unspecified reactive species

M Flavia Barbano,Huiling Wang,Shiliang Zhang,Alexey V Shevelkin,Kevin J Yu,Christopher T Richie,Bing Liu,Suyun Hahn,Rong Ye,Marisela Morales

Frontiers in neuroscience 18:1474617 PubMed39445075

2024

SHARD: an improved method for staining and visualizing multiplex immunofluorescence in optically cleared postmortem human brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Grace A Rosen,Daniel Kirsch,Raymond Nicks,Hunter Kelley,Rebecca Mathias,Kerry A Cormier,Caroline A Kubilus,Bryan Dec,Thor D Stein,Victor E Alvarez,Michael L Alosco,Ann C McKee,Bertrand R Huber

Acta neuropathologica communications 12:132 PubMed39138580

2024

Astrocyte tau deposition in progressive supranuclear palsy is associated with dysregulation of MAPT transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Rosemary J Jackson,Alexandra Melloni,Dustin P Fykstra,Alberto Serrano-Pozo,Leslie Shinobu,Bradley T Hyman

International journal of molecular sciences 24: PubMed37686439

2023

Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Choi,Brenna S Hefley,Sarah E Nicholas,Rebecca L Cunningham,Dimitrios Karamichos

Cell reports 42:113029 PubMed37632750

2023

Lateral preoptic area glutamate neurons relay nociceptive information to the ventral tegmental area.

Applications

Unspecified application

Species

Unspecified reactive species

David J Barker,Shiliang Zhang,Huiling Wang,David J Estrin,Jorge Miranda-Barrientos,Bing Liu,Rucha J Kulkarni,Junia Lara de Deus,Marisela Morales

Heliyon 9:e17907 PubMed37483799

2023

Botulinum toxin type A activates protective autophagy by modulating endoplasmic reticulum stress in hypoxia/reoxygenation-treated endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yinhua Zhao,Huang Lin,Jiaxing Ma,Yue Cui

Journal of chemical neuroanatomy 131:102284 PubMed37164181

2023

Characterization of three cholinergic inputs to the cochlear nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Nichole L Beebe,Yoani N Herrera,William A Noftz,Michael T Roberts,Brett R Schofield

Heliyon 9:e13315 PubMed36747525

2023

Extracellular vesicles from hypoxia-pretreated adipose-derived stem cells regulate hypoxia/reoxygenation-induced human dermal microvascular endothelial apoptosis and autophagy in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

M M Yinhua Zhao,M M Yanyu Shi,Huang Lin

Cancer research communications 2:172-181 PubMed36874403

2022

Stromal FAP Expression is Associated with MRI Visibility and Patient Survival in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Teijo Pellinen,Kevin Sandeman,Sami Blom,Riku Turkki,Annabrita Hemmes,Katja Välimäki,Juho Eineluoto,Anu Kenttämies,Stig Nordling,Olli Kallioniemi,Antti Rannikko,Tuomas Mirtti

Molecular oncology 16:2312-2329 PubMed34890102

2021

E-cadherin is a robust prognostic biomarker in colorectal cancer and low expression is associated with sensitivity to inhibitors of topoisomerase, aurora, and HSP90 in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Jarle Bruun,Peter W Eide,Christian Holst Bergsland,Oscar Bruck,Aud Svindland,Mariliina Arjama,Katja Välimäki,Merete Bjørnslett,Marianne G Guren,Olli Kallioniemi,Arild Nesbakken,Ragnhild A Lothe,Teijo Pellinen
View all publications
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Product promise

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