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AB175739

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed

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(4 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images
Immunoprecipitation - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (AB175739)
  • IP

AbReview41852****

Immunoprecipitation - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (AB175739)

p53 was immunoprecipitated from 7x106 HCT116 (human colon carcinoma cell line) cells with ab26 at 1/150 dilution. Western blot was performed from the immunoprecipitate using anti-p53 antibody. Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) was used as secondary antibody at 1/5000 dilution.

Lane 1 : HCT116 whole cell lysate 10 μg (Input).

Lane 2 : ab207799 IP in etoposide treated HCT116 whole cell lysate.

Lane 3 : ab207799 IP in etoposide treated HCT116 p53-/- whole cell lysate (negative control).

All lanes:

Anti p53 antibody

Secondary

All lanes:

Immunoprecipitation - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) at 1/5000 dilution

Predicted band size: 43 kDa

false

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (AB175739)
  • WB

Lab

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (AB175739)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175739 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed, ab175739 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed, ab175739 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 148:72 PubMed39585417

2024

SMOC1 colocalizes with Alzheimer's disease neuropathology and delays Aβ aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaleah Balcomb,Caitlin Johnston,Tomas Kavanagh,Dominique Leitner,Julie Schneider,Glenda Halliday,Thomas Wisniewski,Margaret Sunde,Eleanor Drummond

Communications biology 6:1183 PubMed37985874

2023

Elevated stress response marks deeply quiescent reserve cells of gastric chief cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Shiokawa,Hiroaki Sakai,Miho Koizumi,Yoshie Okimoto,Yutaro Mori,Yusuke Kanda,Hirokazu Ohata,Hiroaki Honda,Koji Okamoto

International journal of molecular sciences 24: PubMed37833954

2023

L. Indica Seed Coat Ameliorated Concanavalin A-Induced Acute Hepatitis in Mice via MDM2/p53 and PKCα/MAPK1 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiye Zhao,Ye Li,Shancheng Guo,Yuxu Chen,Haiaolong Yin,Yaxian Li,Guiguang Cheng,Lei Tian

iScience 26:108010 PubMed37829206

2023

Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Kameyama,Muneaki Miyata,Hajime Shiotani,Jun Adachi,Soichiro Kakuta,Yasuo Uchiyama,Kiyohito Mizutani,Yoshimi Takai
View all publications

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