Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
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Publications
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- Communications biology 6:11832023Elevated stress response marks deeply quiescent reserve cells of gastric chief cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Shiokawa,Hiroaki Sakai,Miho Koizumi,Yoshie Okimoto,Yutaro Mori,Yusuke Kanda,Hirokazu Ohata,Hiroaki Honda,Koji OkamotoPubMed 37985874
- International journal of molecular sciences 24:2023L. Indica Seed Coat Ameliorated Concanavalin A-Induced Acute Hepatitis in Mice via MDM2/p53 and PKCα/MAPK1 Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiye Zhao,Ye Li,Shancheng Guo,Yuxu Chen,Haiaolong Yin,Yaxian Li,Guiguang Cheng,Lei TianPubMed 37833954
- iScience 26:1080102023Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeshi Kameyama,Muneaki Miyata,Hajime Shiotani,Jun Adachi,Soichiro Kakuta,Yasuo Uchiyama,Kiyohito Mizutani,Yoshimi TakaiPubMed 37829206
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 750
- Excitation/Emission
- Ex: 749nm, Em: 775nm
- Applications
- WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 750
- Excitation/Emission
- Ex: 749nm, Em: 775nm
- Applications
- WB
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed, ab175739 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed, ab175739 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 overnight at 4°C. Antibody binding was detected using ab175739 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
Immunoprecipitation - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739)
p53 was immunoprecipitated from 7x106 HCT116 (human colon carcinoma cell line) cells with Anti-p53 antibody [PAb 240] ab26 at 1/150 dilution. Western blot was performed from the immunoprecipitate using anti-p53 antibody. Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) was used as secondary antibody at 1/5000 dilution.
Lane 1: HCT116 whole cell lysate 10 μg (Input).Lane 2: Anti-Adipose Triglyceride Lipase antibody [EPR19650] ab207799 IP in etoposide treated HCT116 whole cell lysate.
Lane 3: Anti-Adipose Triglyceride Lipase antibody [EPR19650] ab207799 IP in etoposide treated HCT116 p53-/- whole cell lysate (negative control).
All lanes: Anti p53 antibody
SecondaryAll lanes: Immunoprecipitation - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) at 1/5000 dilution
Predicted band size: 43 kDa
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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