JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175782

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790)

4

(1 Review)

|

(4 Publications)

Suitable for WB. Cited in 4 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images
Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) (AB175782)
  • WB

Lab

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) (AB175782)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175782 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175782) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 790

Excitation/Emission

Ex: 782nm, Em: 805nm

Applications

WB

applications

Clonality

Polyclonal

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

You may be interested in:

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

5

5 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

Product details

We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790), ab175782 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790), ab175782 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Endocrine connections 12: PubMed37855365

2023

Cyclic AMP mediates ovine cumulus-oocyte gap junctional function via balancing connexin 43 expression and phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yufen Zhao,Erge Namei,Bingxue Yang,Xiangnan Bao,Wei Sun,Gerile Subudeng,Guifang Cao,Haijun Li,Gui Wang

Endocrine connections : PubMed37721218

2023

Cyclic AMP mediates ovine cumulus-oocyte gap junctional function via balancing connexin43 expression and phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yufen Zhao,Erge Namei,Bingxue Yang,Xiangnan Bao,Wei Sun,Gerile Subudeng,Guifang Cao,Haijun Li,Gui Wang

Scientific reports 13:10288 PubMed37355753

2023

Hyperphagia of female UCP1-deficient mice blunts anti-obesity effects of FGF21.

Applications

Unspecified application

Species

Unspecified reactive species

Marlou Klein Hazebroek,Rutger Laterveer,Maria Kutschke,Vida Ramšak Marčeta,Clarissa S Barthem,Susanne Keipert

Reproduction (Cambridge, England) 160:853-862 PubMed33112787

2020

The cAMP-ERK1/2 signaling pathway regulates urokinase-type plasminogen activator-induced bovine granulosa cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yufen Zhao,Boyang Yu,Xinyu Liu,Jitu Hu,Yanyan Yang,Erge Namei,Bingxue Yang,Yue Bai,Yinghong Qian,Haijun Li
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Associated Products

Select an associated product type
Alternative Product
Secondary Antibodies

AB150105

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)

secondary-antibodies

donkey-mouse-igg-h-l-alexa-fluor-488-ab150105

4

(5 reviews)

Alternative Product
Secondary Antibodies

AB150106

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)

secondary-antibodies

donkey-mouse-igg-h-l-alexa-fluor-555-ab150106

5

(1 reviews)

Alternative Product
Secondary Antibodies

AB150107

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)

secondary-antibodies

donkey-mouse-igg-h-l-alexa-fluor-647-ab150107

5

(2 reviews)

Alternative Product
Secondary Antibodies

AB150108

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)

secondary-antibodies

donkey-mouse-igg-h-l-alexa-fluor-594-ab150108

5

(1 reviews)

Alternative Product
Secondary Antibodies

AB175658

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)

secondary-antibodies

donkey-mouse-igg-h-l-alexa-fluor-405-ab175658

5

(1 reviews)

Alternative Product
Secondary Antibodies

AB175700

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

secondary-antibodies

donkey-mouse-igg-h-l-alexa-fluor-568-preadsorbed-ab175700

0

(0 reviews)

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com