Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
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Publications
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- Journal of anatomy 235:452-4672019Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKim V Korrell et. al.PubMed 30901089
- Cerebral cortex (New York, N.Y. : 1991) 23:1473-832012Molecular diversity of early-born subplate neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Hoerder-Suabedissen et. al.PubMed 22628460
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Horse, Cow, Human
- Conjugation
- Biotin
- Applications
- WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA
- Clonality
- Polyclonal
Immunogen
- Database link P01868
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/2000 - 1/10000 | Notes - |
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/20000 - 1/100000 | Notes - |
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Horse, Cow, Human
- Conjugation
- Biotin
- Applications
- WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Immunogen
- Database link P01868
- Specificity
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Anti-Mouse IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060)
Western blot analysis of ab7060 used as a secondary antibody at 1/1000 dilution for 60 min at RT.
All lanes: Mouse IgG at 50 ng
SecondaryAll lanes: Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060) at 1/1000 dilution
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Product protocols
- Visit the general protocols
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