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Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

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Images

Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (AB7060), expandable thumbnail

Publications

Key facts

Host species
Donkey
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rat, Sheep, Rabbit, Horse, Cow, Human
Conjugation
Biotin
Applications
WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA
Clonality
Polyclonal

Immunogen

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
1/2000 - 1/10000
Notes

-

Application
Dot
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
-
Notes

-

Application
IM
Reactivity
Expected
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Expected
Dilution info
1/1000 - 1/5000
Notes

-

Application
ELISA
Reactivity
Expected
Dilution info
1/20000 - 1/100000
Notes

-

Additional Targets

Alternative names

Recommended products

Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Key facts

Description
Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed
Host species
Donkey
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rat, Sheep, Rabbit, Horse, Cow, Human
Conjugation
Biotin
Applications
WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA
Clonality
Polyclonal
Pre-adsorbed
Yes
Immunogen
Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

Anti-Mouse IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060), expandable thumbnail

    Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060)

    Western blot analysis of ab7060 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

    All lanes: Mouse IgG at 50 ng

    Secondary

    All lanes: Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060) at 1/1000 dilution

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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