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AB7060

Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed

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(2 Publications)

Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images
Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (AB7060)
  • WB

Supplier Data

Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (AB7060)

Western blot analysis of ab7060 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060) at 1/1000 dilution

false

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission
Applications

ELISA, WB, IM, Dot, ICC/IF, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Immunogen

Native Full Length Protein corresponding to Mouse Ighg1.

P01868

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/20000 - 1/100000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-Mouse IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of anatomy 235:452-467 PubMed30901089

2019

Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Kim V Korrell,Jolande Disser,Kristina Parley,Auguste Vadisiute,Maï-Carmen Requena-Komuro,Harriet Fodder,Charlotte Pollart,Graham Knott,Zoltán Molnár,Anna Hoerder-Suabedissen

Cerebral cortex (New York, N.Y. : 1991) 23:1473-83 PubMed22628460

2012

Molecular diversity of early-born subplate neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Hoerder-Suabedissen,Zoltán Molnár
View all publications
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