Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 18 publications.
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- Cells 12:2023An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría José Castellanos-Montiel,Mathilde Chaineau,Anna Kristyna Franco-Flores,Ghazal Haghi,Dulce Carrillo-Valenzuela,Wolfgang E Reintsch,Carol X-Q Chen,Thomas M DurcanPubMed 36831212
- Birth defects research 114:1210-12282022A novel human stem cell-based biomarker assay for in vitro assessment of developmental toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmer Jamalpoor,Sabine Hartvelt,Myrto Dimopoulou,Tom Zwetsloot,Inger Brandsma,Peter I Racz,Torben Osterlund,Giel HendriksPubMed 35289129
- International journal of molecular sciences 22:2021Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton's Jelly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng-Fong Chen,Yi-Chun Chen,Yu-Show Fu,Shang-Wen Tsai,Po-Kuei Wu,Chao-Ming Chen,Ming-Chau Chang,Wei-Ming ChenPubMed 34445692
- Frontiers in synaptic neuroscience 12:5817142021Cyfip1 Regulates SynGAP1 at Hippocampal Synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhishek Sahasrabudhe,Fatema Begum,Christopher A Guevara,Chenel Morrison,Kuangfu Hsiao,Nebojsa Kezunovic,Ozlem Bozdagi-Gunal,Deanna L BensonPubMed 33613257
- Diabetology & metabolic syndrome 12:662020Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Jara et. al.PubMed 32774470
- Fluids and barriers of the CNS 17:172020Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Dolgodilina,Simone M Camargo,Eva Roth,Brigitte Herzog,Virginia Nunes,Manuel Palacín,Francois VerreyPubMed 32046769
- Theranostics 9:7403-74162019Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic strategy for myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiqi Lv et. al.PubMed 31695776
- Stem cell research & therapy 10:1842019Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNi Li et. al.PubMed 31234925
- Molecular medicine reports 19:2774-27802019Resveratrol alleviates hypoxia/reoxygenation injury‑induced mitochondrial oxidative stress in cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Li et. al.PubMed 30816439
- In vitro cellular & developmental biology. Animal 55:17-242018Butyric acid induces spontaneous adipocytic differentiation of porcine bone marrow-derived mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenedetta Tugnoli,Chiara Bernardini,Monica Forni,Andrea Piva,Chad H Stahl,Ester GrilliPubMed 30456456
Key facts
- Host species
Donkey
- Target species
Mouse
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Conjugation
DyLight® 488
- Applications
IHC-P, Flow Cyt, ICC/IF
- Clonality
Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Targets
Ig gamma-1 chain C region, membrane-bound form
Alternative names
Ig gamma-1 chain C region secreted form, Ighg1, Igh-4
Recommended products
Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 18 publications.
Alternative names
Ig gamma-1 chain C region secreted form, Ighg1, Igh-4
Key facts
- Description
Donkey Anti-Mouse IgG H&L (DyLight® 488)
- Host species
Donkey
- Target species
Mouse
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Conjugation
DyLight® 488
- Applications
IHC-P, Flow Cyt, ICC/IF
- Clonality
Polyclonal
- Pre-adsorbed
No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Product promise
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1 product image
This image is courtesy of a customer review submitted by Charles Pallangyo.Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
Anti-alpha smooth muscle Actin antibody [1A4] ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.
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