AB96875

Donkey Anti-Mouse IgG H&L (DyLight® 488)

Name: Donkey Anti Mouse (IgG) secondary antibody DyLight® 488 (ab96875) | Abcam
Brand: Abcam Limited
SKU: AB96875
Price: 265 USD
Availability: InStock

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(21 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 21 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (AB96875)

ICC/IF

AbReview36904****

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (AB96875)

ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.

This image is courtesy of an Abreview submitted by Charles Pallangyo.

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 148:2522-2536 PubMed39823578

2025

Spreading depolarization triggers pro- and anti-inflammatory signalling: a potential link to headache.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Kaya,Nevin Belder,Melike Sever-Bahcekapili,Şefik Evren Erdener,Buket Dönmez-Demir,Canan Bağcı,Merve Nur Köroğlu,Kaya Bilguvar,Turgay Dalkara

iScience 27:109166 PubMed38433895

2024

Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to hypoactivity and synaptic abnormalities in human iPSC-derived motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Lépine,Angela Nauleau-Javaudin,Eric Deneault,Carol X-Q Chen,Narges Abdian,Anna Krystina Franco-Flores,Ghazal Haghi,María José Castellanos-Montiel,Gilles Maussion,Mathilde Chaineau,Thomas Martin Durcan

Experimental and therapeutic medicine 27:72 PubMed38234625

2024

Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Wei Zhang,Xin-Yu Qiao,Chong Liu,Ji-Ji Dao,Chen-Meng Qiao,Chun Cui,Yan-Qin Shen,Wei-Jiang Zhao

Cells 12: PubMed36831212

2023

An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

María José Castellanos-Montiel,Mathilde Chaineau,Anna Kristyna Franco-Flores,Ghazal Haghi,Dulce Carrillo-Valenzuela,Wolfgang E Reintsch,Carol X-Q Chen,Thomas M Durcan

Birth defects research 114:1210-1228 PubMed35289129

2022

A novel human stem cell-based biomarker assay for in vitro assessment of developmental toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Amer Jamalpoor,Sabine Hartvelt,Myrto Dimopoulou,Tom Zwetsloot,Inger Brandsma,Peter I Racz,Torben Osterlund,Giel Hendriks

International journal of molecular sciences 22: PubMed34445692

2021

Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton's Jelly.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Fong Chen,Yi-Chun Chen,Yu-Show Fu,Shang-Wen Tsai,Po-Kuei Wu,Chao-Ming Chen,Ming-Chau Chang,Wei-Ming Chen

Frontiers in synaptic neuroscience 12:581714 PubMed33613257

2021

Cyfip1 Regulates SynGAP1 at Hippocampal Synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Sahasrabudhe,Fatema Begum,Christopher A Guevara,Chenel Morrison,Kuangfu Hsiao,Nebojsa Kezunovic,Ozlem Bozdagi-Gunal,Deanna L Benson

Diabetology & metabolic syndrome 12:66 PubMed32774470

2020

Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Jara,Felipe Oyarzun-Ampuero,Flavio Carrión,Esteban González-Echeverría,Claudio Cappelli,Pablo Caviedes

Fluids and barriers of the CNS 17:17 PubMed32046769

2020

Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Dolgodilina,Simone M Camargo,Eva Roth,Brigitte Herzog,Virginia Nunes,Manuel Palacín,Francois Verrey

Theranostics 9:7403-7416 PubMed31695776

2019

Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic strategy for myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqi Lv,Qingju Li,Ling Zhang,Yingchao Wang,Zhiwei Zhong,Jing Zhao,Xiaoxiao Lin,Jingyi Wang,Keyang Zhu,Changchen Xiao,Changle Ke,Shuhan Zhong,Xianpeng Wu,Jinghai Chen,Hong Yu,Wei Zhu,Xiang Li,Ben Wang,Ruikang Tang,Jian'an Wang,Jinyu Huang,Xinyang Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Mouse IgG H&L (DyLight® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (AB96875)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (21)

Product promise