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Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (AB98794), expandable thumbnail

Publications

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Conjugation

DyLight® 488

Applications

IHC-P, Flow Cyt, ICC/IF, WB

Clonality

Polyclonal

Reactivity data

Application

IHC-P

Reactivity

Reacts

Dilution info

1/40.00000 - 1/400.00000

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/40.00000 - 1/500.00000

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/1000.00000 - 1/20000.00000

Notes

5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Associated Products

Select an associated product type

6 products for Alternative Product

Targets

Ig gamma-1 chain C region, membrane-bound form

Alternative names

Recommended products

Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

Key facts

Description

Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Conjugation

DyLight® 488

Applications

IHC-P, Flow Cyt, ICC/IF, WB

Clonality

Polyclonal

Pre-adsorbed

Yes

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)

    Immunohistochemistry immunofluorescent analysis of human ovarian carcinoma tissue using primary mouse anti-p53 at 1/100 dilution and secondary ab98794 at 1/50 with different lots on left and right panels. Counterstain was DAPI.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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