Suitable for WB, ICC/IF, Flow Cyt, IHC-P. Cited in 14 publications.
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- Neurobiology of disease 164:1056092022Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRémi Bos,Khalil Rihan,Patrice Quintana,Lara El-Bazzal,Nathalie Bernard-Marissal,Nathalie Da Silva,Rosette Jabbour,André Mégarbané,Marc Bartoli,Frédéric Brocard,Valérie DelaguePubMed 34990802
- Cells 9:2020Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIssan Zhang et. al.PubMed 33171886
- International journal of cancer 146:3435-34492019Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTinka Haydn,Sarah Kehr,Dominica Willmann,Eric Metzger,Roland Schüle,Simone FuldaPubMed 31755110
- Theranostics 9:7403-74162019Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic strategy for myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiqi Lv et. al.PubMed 31695776
- Human molecular genetics 28:2378-23942019Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLara El-Bazzal,Khalil Rihan,Nathalie Bernard-Marissal,Christel Castro,Eliane Chouery-Khoury,Jean-Pierre Desvignes,Alexandre Atkinson,Karine Bertaux,Salam Koussa,Nicolas Lévy,Marc Bartoli,André Mégarbané,Rosette Jabbour,Valérie DelaguePubMed 31090908
- Toxicology in vitro : an international journal published in association with BIBRA 58:69-772019Identification of key pathways involved in the toxic response of the cyanobacterial toxin cylindrospermopsin in human hepatic HepaRG cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntoine Huguet,Rachelle Lanceleur,Hélène Quenault,Ludovic Le Hégarat,Valérie FessardPubMed 30905859
- International journal of molecular sciences 20:2019Loss of SR-BI Down-Regulates MITF and Suppresses Extracellular Vesicle Release in Human Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Kinslechner et. al.PubMed 30823658
- Toxins 9:2017Metabolism of the Marine Phycotoxin PTX-2 and Its Effects on Hepatic Xenobiotic Metabolism: Activation of Nuclear Receptors and Modulation of the Phase I Cytochrome P450.
- Developmental cell 41:94-106.e42017Neurexin Restricts Axonal Branching in Columns by Promoting Ephrin Clustering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLina Liu et. al.PubMed 28366281
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- DyLight® 550
- Applications
- WB, ICC/IF, Flow Cyt, IHC-P
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. |
Associated Products
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6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for WB, ICC/IF, Flow Cyt, IHC-P. Cited in 14 publications.
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (DyLight® 550)
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- DyLight® 550
- Applications
- WB, ICC/IF, Flow Cyt, IHC-P
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
DyLight® 550 replaces DyLight® 549.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
Formalin-fixed paraffin-embedded sections of human ovarian carcinoma stained for p53 using a mouse anti-p53 (DO-1) antibody, followed by ab96876 at a 1:100 dilution (5 μg/ml). Counterstained with DAPI (blue).
Courtesy of an anonymous customer reviewWestern blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484) at 1/1000 dilution
- Lane 1 : Rat Hippocampus
- Lane 2 : Rat Hippocampus
- Lane 3 : Rat Hippocampus
Lysates/proteins at 25 µg per lane.Secondary:
- Lane 1 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/5000 dilution
- Lane 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/1000 dilution
- Lane 3 : Other Commercial Dye at 1/1000 dilution
Performed under reducing conditions.
SecondaryAll lanes: Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
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