AB96876

Donkey Anti-Mouse IgG H&L (DyLight® 550)

Name: Donkey Anti Mouse (IgG) secondary antibody DyLight® 550 (ab96876) | Abcam
Brand: Abcam Limited
SKU: AB96876
Price: 265 USD
Availability: InStock

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(17 Publications)

Suitable for WB, ICC/IF, Flow Cyt, IHC-P. Cited in 17 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (AB96876)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (AB96876)

Formalin-fixed paraffin-embedded sections of human ovarian carcinoma stained for p53 using a mouse anti-p53 (DO-1) antibody, followed by ab96876 at a 1 : 100 dilution (5 μg/ml). Counterstained with DAPI (blue).

Unknown

Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (AB96876)

All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution

Lane 1 : Rat Hippocampus
Lane 2 : Rat Hippocampus
Lane 3 : Rat Hippocampus

Lysates/proteins at 25 µg per lane.

Secondary :

Lane 1 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/5000 dilution
Lane 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/1000 dilution
Lane 3 : Other Commercial Dye at 1/1000 dilution

Performed under reducing conditions.

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

false

Courtesy of an anonymous Abreview

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

WB, Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments." } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in plant science 15:1363555 PubMed39011301

2024

SYNAPTOTAGMIN 4 is expressed mainly in the phloem and participates in abiotic stress tolerance in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Ajay Kumar,Miroslav Krausko,Ján Jásik

iScience 27:109166 PubMed38433895

2024

Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to hypoactivity and synaptic abnormalities in human iPSC-derived motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Lépine,Angela Nauleau-Javaudin,Eric Deneault,Carol X-Q Chen,Narges Abdian,Anna Krystina Franco-Flores,Ghazal Haghi,María José Castellanos-Montiel,Gilles Maussion,Mathilde Chaineau,Thomas Martin Durcan

Animal models and experimental medicine 7:523-532 PubMed38372410

2024

A novel mouse model of calcific aortic valve stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ningjing Qian,Yaping Wang,Wangxing Hu,Naifang Cao,Yi Qian,Jinyong Chen,Juan Fang,Dilin Xu,Haochang Hu,Shuangshuang Yang,Dao Zhou,Hanyi Dai,Dongdong Wei,Jian'an Wang,Xianbao Liu

Neurobiology of disease 164:105609 PubMed34990802

2022

Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1.

Applications

Unspecified application

Species

Unspecified reactive species

Rémi Bos,Khalil Rihan,Patrice Quintana,Lara El-Bazzal,Nathalie Bernard-Marissal,Nathalie Da Silva,Rosette Jabbour,André Mégarbané,Marc Bartoli,Frédéric Brocard,Valérie Delague

Methods (San Diego, Calif.) 203:465-477 PubMed34314827

2021

Microfabricated disk technology: Rapid scale up in midbrain organoid generation.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen-Vi Mohamed,Paula Lépine,María Lacalle-Aurioles,Julien Sirois,Meghna Mathur,Wolfgang Reintsch,Lenore K Beitel,Edward A Fon,Thomas M Durcan

Cells 9: PubMed33171886

2020

Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures.

Applications

Unspecified application

Species

Unspecified reactive species

Issan Zhang,Paula Lépine,Chanshuai Han,María Lacalle-Aurioles,Carol X-Q Chen,Rainer Haag,Thomas M Durcan,Dusica Maysinger

International journal of cancer 146:3435-3449 PubMed31755110

2019

Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tinka Haydn,Sarah Kehr,Dominica Willmann,Eric Metzger,Roland Schüle,Simone Fulda

Theranostics 9:7403-7416 PubMed31695776

2019

Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic strategy for myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqi Lv,Qingju Li,Ling Zhang,Yingchao Wang,Zhiwei Zhong,Jing Zhao,Xiaoxiao Lin,Jingyi Wang,Keyang Zhu,Changchen Xiao,Changle Ke,Shuhan Zhong,Xianpeng Wu,Jinghai Chen,Hong Yu,Wei Zhu,Xiang Li,Ben Wang,Ruikang Tang,Jian'an Wang,Jinyu Huang,Xinyang Hu

Human molecular genetics 28:2378-2394 PubMed31090908

2019

Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1.

Applications

Unspecified application

Species

Unspecified reactive species

Lara El-Bazzal,Khalil Rihan,Nathalie Bernard-Marissal,Christel Castro,Eliane Chouery-Khoury,Jean-Pierre Desvignes,Alexandre Atkinson,Karine Bertaux,Salam Koussa,Nicolas Lévy,Marc Bartoli,André Mégarbané,Rosette Jabbour,Valérie Delague

Toxicology in vitro : an international journal published in association with BIBRA 58:69-77 PubMed30905859

2019

Identification of key pathways involved in the toxic response of the cyanobacterial toxin cylindrospermopsin in human hepatic HepaRG cells.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Huguet,Rachelle Lanceleur,Hélène Quenault,Ludovic Le Hégarat,Valérie Fessard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Mouse IgG H&L (DyLight® 550)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (AB96876)

Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (AB96876)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

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Secondary Antibodies

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (17)

Product promise