AB98795

Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed

Name: Donkey Anti Mouse (IgG) secondary antibody preadsorbed DyLight® 550 (ab98795)
Brand: Abcam Limited
SKU: AB98795
Price: 295 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (AB98795)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (AB98795)

Immunofluorescence analysis of FFPE section of human ovarian carcinoma tissue stained for p53 protein with mouse monoclonal anti-p53 (Clone DO-1) antibody at a 1/100 dilution.Red-fluorescent donkey anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight^® 550 Conjugated (ab98795 ) was used as secondary at a 1/100 dilution(5µg/ml). DAPI was used as nuclear staining.

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2303128 PubMed38348560

2024

Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeon Ryoo,Regina Giovanni,Hannah Kimmel,Ishita Jain,Gregory H Underhill

International journal of molecular sciences 22: PubMed34299212

2021

Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species.

Applications

Unspecified application

Species

Unspecified reactive species

Anberitha T Matthews,Hitesh Soni,Katherine E Robinson-Freeman,Theresa A John,Randal K Buddington,Adebowale Adebiyi

Molecular cancer 20:16 PubMed33461590

2021

The inflammatory kinase IKKα phosphorylates and stabilizes c-Myc and enhances its activity.

Applications

Unspecified application

Species

Unspecified reactive species

Bernhard Moser,Bernhard Hochreiter,José Basílio,Viola Gleitsmann,Anja Panhuber,Alan Pardo-Garcia,Bastian Hoesel,Manuel Salzmann,Ulrike Resch,Mamoona Noreen,Johannes A Schmid

Genome research 28:1455-1466 PubMed30166406

2018

Study of mitotic chromatin supports a model of bookmarking by histone modifications and reveals nucleosome deposition patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Elisheva Javasky,Inbal Shamir,Shashi Gandhi,Shawn Egri,Oded Sandler,Scott B Rothbart,Noam Kaplan,Jacob D Jaffe,Alon Goren,Itamar Simon

Acta neuropathologica communications 6:52 PubMed29958544

2018

Phosphorylation of different tau sites during progression of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Joerg Neddens,Magdalena Temmel,Stefanie Flunkert,Bianca Kerschbaumer,Christina Hoeller,Tina Loeffler,Vera Niederkofler,Guenther Daum,Johannes Attems,Birgit Hutter-Paier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (AB98795)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (5)

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