AB7057

Donkey Anti-Mouse IgG H&L (FITC) preadsorbed

Name: Donkey Anti Mouse (IgG) secondary antibody preadsorbed FITC (ab7057) | Abcam
Brand: Abcam Limited
SKU: AB7057
Price: 295 USD
Availability: InStock

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(9 Publications)

Suitable for IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Supplier Data

Western blot - Donkey Anti-Mouse IgG H&L (FITC) preadsorbed (AB7057)

Western blot analysis of ab7057 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (FITC) preadsorbed (ab7057) at 1/1000 dilution

false

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-P, IM, WB, ICC/IF, Flow Cyt, IHC-Fr

applications

Clonality

Polyclonal

Immunogen

Native Full Length Protein corresponding to Mouse Ighg1.

P01868

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2500", "notes":"" }, "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 23:262-278 PubMed38597826

2024

DNMT1-mediated epigenetic suppression of FBXO32 expression promoting cyclin dependent kinase 9 (CDK9) survival and esophageal cancer cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Qiang Song,Bin-Bin Chen,Yong-Mei Jin,Chang-Yong Wang

iScience 26:105885 PubMed36654858

2023

Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Jin,Xiangfeng Chen,Hanyu Zheng,Wanxiong Cai,Xurong Lin,Xiangxing Kong,Yingchun Ni,Jingjia Ye,Xiaodan Li,Luoan Shen,Binjie Guo,Zeinab Abdelrahman,Songlin Zhou,Susu Mao,Yaxian Wang,Chun Yao,Xiaosong Gu,Bin Yu,Zhiping Wang,Xuhua Wang

ACS nano 14:15276-15285 PubMed33164505

2020

Differentiation of Cancer Stem Cells through Nanoparticle Surface Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Geng,John J Amante,Hira L Goel,Xianzhi Zhang,Melanie R Walker,David C Luther,Arthur M Mercurio,Vincent M Rotello

Oncology letters 18:4092-4099 PubMed31516609

2019

Heavy-ion beam irradiation inhibits invasion of tongue squamous cell carcinoma Tca8113 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenghu Feng,Chunqing Li,Qian Zheng,Weigang Mao,Tao Li,Long Xing,Qiang Li

Dalton transactions (Cambridge, England : 2003) 44:4994-5003 PubMed25612290

2015

Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Stina Lindén,Manish Kumar Singh,K David Wegner,Marie Regairaz,François Dautry,François Treussart,Niko Hildebrandt

PLoS pathogens 10:e1003885 PubMed24453977

2014

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

Applications

Unspecified application

Species

Unspecified reactive species

Marco A Ataide,Warrison A Andrade,Dario S Zamboni,Donghai Wang,Maria do Carmo Souza,Bernardo S Franklin,Samir Elian,Flaviano S Martins,Dhelio Pereira,George Reed,Katherine A Fitzgerald,Douglas T Golenbock,Ricardo T Gazzinelli

Cellular and molecular neurobiology 32:1047-57 PubMed22402835

2012

Dual effect of methylglyoxal on the intracellular Ca2+ signaling and neurite outgrowth in mouse sensory neurons.

Applications

ICC/IF

Species

Unspecified reactive species

Beatrice Mihaela Radu,Diana Ionela Dumitrescu,Cosmin Catalin Mustaciosu,Mihai Radu

Human molecular genetics 17:1527-39 PubMed18267959

2008

The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Miranda,Ian MacLeod,Mark J Davies,Juan Pérez,Karin Römisch,Damian C Crowther,David A Lomas

Journal of neurochemistry 95:1015-22 PubMed16190880

2005

Astrocyte-neurone communication following oxygen-glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

S Griffin,J B Clark,L Canevari

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (FITC) preadsorbed

Western blot - Donkey Anti-Mouse IgG H&L (FITC) preadsorbed (AB7057)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Immunogen

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (9)

Product promise