Donkey Anti-Mouse IgG H&L (HRP)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (HRP) (AB6820)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6820, 1/2000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

PubMed

Western blot - Donkey Anti-Mouse IgG H&L (HRP) (AB6820)

Detection of protein levels in LIN-5 mutants.

(Panel A) Western blots of lysates of yeast clones containing the indicated LIN-5 expression constructs, probed for LIN-5 and tubulin (loading control) levels.

Protein samples were separated on gradient acrylamide gels and subjected to western blotting on polyvinylidene difluoride membrane. Membranes were blocked with 5% skim milk in PBST for 1 hour at room temperature, or overnight at 4°C for stripped blots. For protein detection, primary antibodies used in this study were : mouse anti–LIN-5 (1 : 1000) and rabbit anti-Tubulin (1 : 1000, Abcam) for stripped blots. Secondary antibodies used were : donkey anti-mouse HRP (1 : 5000, Abcam, ab6820) and goat anti-rabbit HRP (1 : 5000).

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (HRP) (ab6820)

false

Portegijs et al PLoS Genet. 2016 Oct 6;12(10):e1006291. doi: 10.1371/journal.pgen.1006291. eCollection 2016 Oct. Fig S5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Supplier Data

Western blot - Donkey Anti-Mouse IgG H&L (HRP) (AB6820)

Western blot analysis of ab6820 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (HRP) (ab6820) at 1/1000 dilution

false