AB7061

Donkey Anti-Mouse IgG H&L (HRP) preadsorbed

Name: Donkey Anti Mouse (IgG) secondary antibody preadsorbed HRP (ab7061) | Abcam
Brand: Abcam Limited
SKU: AB7061

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(11 Publications)

Suitable for Dot, IHC-Fr, IM, IHC-P, WB, ELISA, ICC. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Supplier Data

Western blot - Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (AB7061)

Western blot analysis of ab7061 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (ab7061) at 1/1000 dilution

false

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Human, Hamster

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ELISA, WB, IM, Dot, IHC-P, IHC-Fr, ICC

applications

Clonality

Polyclonal

Immunogen

Native Full Length Protein corresponding to Mouse Ighg1.

P01868

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/20000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/20000 - 1/80000", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Mouse IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4055 PubMed40307206

2025

Allelic effects on KLHL17 expression underlie a pancreatic cancer genome-wide association signal at chr1p36.33.

Applications

Unspecified application

Species

Unspecified reactive species

Katelyn E Connelly,Katherine Hullin,Ehssan Abdolalizadeh,Jun Zhong,Daina Eiser,Aidan O'Brien,Irene Collins,Sudipto Das,Gerard Duncan,Stephen J Chanock,Rachael Z Stolzenberg-Solomon,Alison P Klein,Brian M Wolpin,Jason W Hoskins,Thorkell Andresson,Jill P Smith,Laufey T Amundadottir

Neuroscience applied 3:104070 PubMed40656072

2024

An investigation on the alterations in Wnt signaling in ADHD across developmental stages.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Monet Walter,Cristine Marie Yde Ohki,Michelle Rickli,Lukasz Smigielski,Susanne Walitza,Edna Grünblatt

Molecular brain 17:13 PubMed38413970

2024

Crucial role of TFAP2B in the nervous system for regulating NREM sleep.

Applications

Unspecified application

Species

Unspecified reactive species

Ayaka Nakai,Mitsuaki Kashiwagi,Tomoyuki Fujiyama,Kanako Iwasaki,Arisa Hirano,Hiromasa Funato,Masashi Yanagisawa,Takeshi Sakurai,Yu Hayashi

Nature biotechnology 41:1229-1238 PubMed36658341

2023

Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sangwook Oh,Xuming Mao,Silvio Manfredo-Vieira,Jinmin Lee,Darshil Patel,Eun Jung Choi,Andrea Alvarado,Ebony Cottman-Thomas,Damian Maseda,Patricia Y Tsao,Christoph T Ellebrecht,Sami L Khella,David P Richman,Kevin C O'Connor,Uri Herzberg,Gwendolyn K Binder,Michael C Milone,Samik Basu,Aimee S Payne

American journal of human genetics 108:1852-1865 PubMed34559995

2021

A 584 bp deletion in CTRB2 inhibits chymotrypsin B2 activity and secretion and confers risk of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley Jermusyk,Jun Zhong,Katelyn E Connelly,Naomi Gordon,Sumeth Perera,Ehssan Abdolalizadeh,Tongwu Zhang,Aidan O'Brien,Jason W Hoskins,Irene Collins,Daina Eiser,Chen Yuan,Harvey A Risch,Eric J Jacobs,Donghui Li,Mengmeng Du,Rachael Z Stolzenberg-Solomon,Alison P Klein,Jill P Smith,Brian M Wolpin,Stephen J Chanock,Jianxin Shi,Gloria M Petersen,Christopher J Westlake,Laufey T Amundadottir

Journal of cellular and molecular medicine 25:3437-3448 PubMed33675119

2021

Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Jiang,Wenjing Xu,Zhenzhen Chen,Changting Cui,Xiaofang Fan,Jun Cai,Yongsheng Gong,Bin Geng

eNeuro 7: PubMed32321771

2020

Progressive Changes in Sleep and Its Relations to Amyloid-β Distribution and Learning in Single Knock-In Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sakura Eri B Maezono,Mika Kanuka,Chika Tatsuzawa,Miho Morita,Taizo Kawano,Mitsuaki Kashiwagi,Pimpimon Nondhalee,Masanori Sakaguchi,Takashi Saito,Takaomi C Saido,Yu Hayashi

Experimental and therapeutic medicine 18:4313-4321 PubMed31777538

2019

Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Luyifei Li,Ling Jing,Jiyi Zhao,Jiachen Lv,Wen Yang,Weimin Li,Lijun Zhou

British journal of pharmacology 176:3180-3192 PubMed31140595

2019

Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine γ lyase S-sulfhydration in ApoE-knockout atherosclerotic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhui Fan,Fengjiao Zheng,Shuangyue Li,Cangting Cui,Shan Jiang,Jun Zhang,Jun Cai,Qinghua Cui,Jichun Yang,Xinjing Tang,Guoheng Xu,Bin Geng

Journal of thrombosis and haemostasis : JTH 14:1298-307 PubMed26990752

2016

Soluble analog of ApoER2 targeting beta2-glycoprotein I in immune complexes counteracts hypertension in lupus-prone mice with spontaneous antiphospholipid syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

A Kolyada,Q Ke,I Karageorgos,P Mahlawat,D A Barrios,P M Kang,N Beglova

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Mouse IgG H&L (HRP) preadsorbed

Western blot - Donkey Anti-Mouse IgG H&L (HRP) preadsorbed (AB7061)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Immunogen

Specificity

Alternative Products

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (11)

Product promise