AB7003

Donkey Anti-Mouse IgG H&L (PE) preadsorbed

Name: Donkey Anti Mouse (IgG) secondary antibody preadsorbed PE (ab7003) | Abcam
Brand: Abcam Limited
SKU: AB7003

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(10 Publications)

Suitable for IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

3 Images

Flow Cytometry - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

Flow Cyt

Unknown

Flow Cytometry - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

ab7003 was used with the primary antibody ab33315 in Flow Cyt. See Abreview on ab33315.

Supplier Data

Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

Dot blot analysis of ab7003. Imaged with BioRad ChemiDoc, Rhodamine filter.

All lanes:

Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003) at 1 µg/µL

Lane 1:

Mouse IgG at 100 ng

Lane 2:

Mouse IgG at 33.3 ng

Lane 3:

Mouse IgG at 11.1 ng

Lane 4:

Mouse IgG at 3.7 ng

Lane 5:

Mouse IgG at 1.23 ng

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Supplier Data

Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

Dot blot analysis of ab7003.

All lanes:

Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)

Lane 1:

Mouse IgG at 100 ng

Lane 2:

Mouse IgG at 33.3 ng

Lane 3:

Mouse IgG at 11.1 ng

Lane 4:

Mouse IgG at 3.7 ng

Lane 5:

Mouse IgG at 1.23 ng

Secondary

All lanes:

F(ab')2 Donkey Anti-Mouse IgG Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins at 1/1000 dilution

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Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

Flow Cyt, IM, ICC/IF, Dot, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"" }, "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 12:1347406 PubMed38694622

2024

Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Zheng,Tong Wu,Feifei Wang,Huazhi Li,Hongyi Tang,Xinyu Cui,Cuiying Li,Yixiang Wang,Jiuhui Jiang

Frontiers in immunology 13:1049900 PubMed36457999

2022

Nectin4 is a potential therapeutic target for asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Pureun-Haneul Lee,Seon Muk Choi,Min Hyeok An,Da Yeon Hwang,Shinhee Park,Ae Rin Baek,An-Soo Jang

Cells 10: PubMed34831174

2021

Long-Term Transplant Effects of iPSC-RPE Monolayer in Immunodeficient RCS Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Deepthi S Rajendran Nair,Danhong Zhu,Ruchi Sharma,Juan Carlos Martinez Camarillo,Kapil Bharti,David R Hinton,Mark S Humayun,Biju B Thomas

Stem cell research & therapy 12:296 PubMed34016181

2021

Strontium ranelate promotes chondrogenesis through inhibition of the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yu,Yan Liu,Xiangwen Yang,Jiajing He,Fan Zhang,Qun Zhong,Xiaojing Guo

Pharmaceuticals (Basel, Switzerland) 13: PubMed32645833

2020

Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rania Harati,Mohammad G Mohammad,Abdelaziz Tlili,Raafat A El-Awady,Rifat Hamoudi

OncoTargets and therapy 11:5047-5057 PubMed30174443

2018

Analysis of the clinical significance of DCLK1 colorectal cancer using novel monoclonal antibodies against DCLK1.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqi Dai,Yunlong Hu,Fulian Lv,Tatsuhiko Ozawa,Xin Sun,Jingjing Huang,Xiaojian Han,Hiroyuki Kishi,Atsushi Muraguchi,Aishun Jin

Biomacromolecules 18:201-209 PubMed28001364

2016

In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Sangeeta R Banerjee,Catherine A Foss,Allen Horhota,Mrudula Pullambhatla,Kevin McDonnell,Stephen Zale,Martin G Pomper

Oncology letters 10:941-945 PubMed26622600

2015

Effects of the novel heat shock protein 90 inhibitor AUY922 in renal cell carcinoma ACHN and 786-O cells.

Applications

Species

Unspecified reactive species

Jingjuan Zhu,Yanbiao Zhu,Weiwei Qi,Wensheng Qiu

Molecular medicine reports 12:2451-6 PubMed25955495

2015

Role of the novel HSP90 inhibitor AUY922 in hepatocellular carcinoma: Potential for therapy.

Applications

Species

Unspecified reactive species

Wei Cheng,Aimudula Ainiwaer,Lei Xiao,Qian Cao,Ge Wu,Ying Yang,Rui Mao,Yongxing Bao

The European journal of neuroscience 39:1234-44 PubMed24713002

2014

Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications.

Applications

Flow Cyt

Species

Unspecified reactive species

Lucile Marion-Poll,Enrica Montalban,Annie Munier,Denis Hervé,Jean-Antoine Girault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (PE) preadsorbed

Flow Cytometry - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (AB7003)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (10)

Product promise