Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Suitable for IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4
- Host species
Donkey
- Target species
Mouse
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Applications
IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt
- Clonality
Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes - |
Images
Recommended products
Suitable for IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4
Key facts
- Description
Donkey Anti-Mouse IgG H&L (PE) preadsorbed
- Host species
Donkey
- Target species
Mouse
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Applications
IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt
- Clonality
Polyclonal
- Pre-adsorbed
Yes
- Specificity
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Purification technique
Affinity purification Immunogen
- Purification notes
F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com