Suitable for IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
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Publications
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- Frontiers in immunology 13:10499002022Nectin4 is a potential therapeutic target for asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPureun-Haneul Lee,Seon Muk Choi,Min Hyeok An,Da Yeon Hwang,Shinhee Park,Ae Rin Baek,An-Soo JangPubMed 36457999
- Cells 10:2021Long-Term Transplant Effects of iPSC-RPE Monolayer in Immunodeficient RCS Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepthi S Rajendran Nair,Danhong Zhu,Ruchi Sharma,Juan Carlos Martinez Camarillo,Kapil Bharti,David R Hinton,Mark S Humayun,Biju B ThomasPubMed 34831174
- Stem cell research & therapy 12:2962021Strontium ranelate promotes chondrogenesis through inhibition of the Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Yu,Yan Liu,Xiangwen Yang,Jiajing He,Fan Zhang,Qun Zhong,Xiaojing GuoPubMed 34016181
- Pharmaceuticals (Basel, Switzerland) 13:2020Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania Harati et. al.PubMed 32645833
- OncoTargets and therapy 11:5047-50572018Analysis of the clinical significance of DCLK1 colorectal cancer using novel monoclonal antibodies against DCLK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianqi Dai et. al.PubMed 30174443
- Biomacromolecules 18:201-2092016In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSangeeta R Banerjee,Catherine A Foss,Allen Horhota,Mrudula Pullambhatla,Kevin McDonnell,Stephen Zale,Martin G PomperPubMed 28001364
- Oncology letters 10:941-9452015Effects of the novel heat shock protein 90 inhibitor AUY922 in renal cell carcinoma ACHN and 786-O cells.
- Molecular medicine reports 12:2451-62015Role of the novel HSP90 inhibitor AUY922 in hepatocellular carcinoma: Potential for therapy.
- The European journal of neuroscience 39:1234-442014Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications.Applications:Flow CytReactive species:Unspecified reactive speciesLucile Marion-Poll,Enrica Montalban,Annie Munier,Denis Hervé,Jean-Antoine GiraultPubMed 24713002
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt, Dot
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/100 - 1/250 | Notes - |
Application Flow Cyt | Reactivity Expected | Dilution info 1/100 - 1/250 | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (PE) preadsorbed
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- IHC-Fr, IHC-P, IM, ICC/IF, Flow Cyt, Dot
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)
Dot blot analysis of ab7003.
All lanes: Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)
Lane 1: Mouse IgG at 100 ng
Lane 2: Mouse IgG at 33.3 ng
Lane 3: Mouse IgG at 11.1 ng
Lane 4: Mouse IgG at 3.7 ng
Lane 5: Mouse IgG at 1.23 ng
SecondaryAll lanes: F(ab')2 Donkey Anti-Mouse IgG Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins at 1/1000 dilution
Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)
Dot blot analysis of ab7003. Imaged with BioRad ChemiDoc, Rhodamine filter.
All lanes: Dot Blot - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003) at 1 µg/µL
Lane 1: Mouse IgG at 100 ng
Lane 2: Mouse IgG at 33.3 ng
Lane 3: Mouse IgG at 11.1 ng
Lane 4: Mouse IgG at 3.7 ng
Lane 5: Mouse IgG at 1.23 ng
Flow Cytometry - Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)
ab7003 was used with the primary antibody Anti-CD151 antibody [11G5a] ab33315 in Flow Cyt. See Abreview on Anti-CD151 antibody [11G5a] ab33315.
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