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AB7056

Donkey Anti-Mouse IgG H&L preadsorbed

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(2 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, ELISA, WB, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Applications

IHC-P, WB, ICC/IF, Conjugation, IHC-Fr, ID, ELISA, IP

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA at less than 1.0% cross reactivity against IgG proteins purified from the species listed above.

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Alternative Products

Secondary Antibodies

AB150105

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150105)

  • 4
  • 5
Secondary Antibodies

AB150106

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)

  • 5
  • 1
Secondary Antibodies

AB150107

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)

Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB150107)

  • 5
  • 2
Secondary Antibodies

AB150108

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150108)

  • 5
  • 1
Secondary Antibodies

AB175658

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

  • 5
  • 1
Secondary Antibodies

AB175700

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175700)

  • 0
  • 0

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Primary Antibodies

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AB32503

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/30000 - 1/120000", "notes":"<p></p>" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 113:E4143-50 PubMed27382182

2016

In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.

Applications

WB

Species

Unspecified reactive species

Xuanye Wu,Nils Schneider,Alina Platen,Indranil Mitra,Matthias Blazek,Roland Zengerle,Roland Schüle,Matthias Meier

Dalton transactions (Cambridge, England : 2003) 44:4994-5003 PubMed25612290

2015

Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Stina Lindén,Manish Kumar Singh,K David Wegner,Marie Regairaz,François Dautry,François Treussart,Niko Hildebrandt
View all publications
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Product promise

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