AB175649

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

Name: Donkey Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 405
Brand: Abcam Limited
SKU: AB175649
Rating: 5 (3 reviews)

(3 Reviews)

(36 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 36 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175649, Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed, used at 2μg/ml for 1h at room temperature. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10713 PubMed39715766

2024

Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eirini Bellou,Agata P Zielinska,Eike Urs Mönnich,Nina Schweizer,Antonio Z Politi,Antonina Wellecke,Claus Sibold,Andreas Tandler-Schneider,Melina Schuh

Nature communications 15:9337 PubMed39472435

2024

Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ying Huang,Yan-Zhou Chen,Chuang Zhao,Xin-Nan Zheng,Kai Yu,Jia-Xing Yue,Huai-Qiang Ju,Yan-Xia Shi,Lin Tian

Cell 187:3427-3444.e21 PubMed38733990

2024

Spinal projecting neurons in rostral ventromedial medulla co-regulate motor and sympathetic tone.

Applications

Unspecified application

Species

Unspecified reactive species

Zicong Zhang,Junfeng Su,Jing Tang,Leeyup Chung,Jessica C Page,Carla C Winter,Yuchu Liu,Evgenii Kegeles,Sara Conti,Yu Zhang,Jason Biundo,Joshua I Chalif,Charles Y Hua,Zhiyun Yang,Xue Yao,Yang Yang,Shuqiang Chen,Jan M Schwab,Kuan Hong Wang,Chinfei Chen,Michael J Prerau,Zhigang He

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00346 PubMed38493058

2024

Neuroprotective effects of niclosamide on disease progression via inflammatory pathways modulation in SOD1-G93A and FUS-associated amyotrophic lateral sclerosis models.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Milani,Ilaria Della Valle,Simona Rossi,Paola Fabbrizio,Cassandra Margotta,Giovanni Nardo,Mauro Cozzolino,Nadia D'Ambrosi,Savina Apolloni

Cell reports 43:113801 PubMed38363678

2024

DLK signaling in axotomized neurons triggers complement activation and loss of upstream synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Asghari Adib,Jennifer L Shadrach,Lauren Reilly-Jankowiak,Manish K Dwivedi,Abigail E Rogers,Shameena Shahzad,Ryan Passino,Roman J Giger,Brian A Pierchala,Catherine A Collins

Experimental neurobiology 32:387-394 PubMed38196134

2024

Distinct Role of Parvalbumin Expressing Neurons in the Reticular Thalamic Nucleus in Nociception.

Applications

Unspecified application

Species

Unspecified reactive species

Sanggeon Park,Jeiwon Cho,Yeowool Huh

Science advances 9:eadd6480 PubMed37595032

2023

Organoid cell fate dynamics in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Zheng,Max A Betjes,Pascal Ender,Yvonne J Goos,Guizela Huelsz-Prince,Hans Clevers,Jeroen S van Zon,Sander J Tans

Cells 12: PubMed37048088

2023

Intramuscular IL-10 Administration Enhances the Activity of Myogenic Precursor Cells and Improves Motor Function in ALS Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Fabbrizio,Cassandra Margotta,Jessica D'Agostino,Giuseppe Suanno,Lorenzo Quetti,Caterina Bendotti,Giovanni Nardo

Inflammation and regeneration 43:19 PubMed36895050

2023

Immune-mediated myogenesis and acetylcholine receptor clustering promote a slow disease progression in ALS mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Cassandra Margotta,Paola Fabbrizio,Marco Ceccanti,Chiara Cambieri,Gabriele Ruffolo,Jessica D'Agostino,Maria Chiara Trolese,Pierangelo Cifelli,Veronica Alfano,Christian Laurini,Silvia Scaricamazza,Alberto Ferri,Gianni Sorarù,Eleonora Palma,Maurizio Inghilleri,Caterina Bendotti,Giovanni Nardo

eLife 11: PubMed36445322

2022

Mother cells control daughter cell proliferation in intestinal organoids to minimize proliferation fluctuations.

Applications

Unspecified application

Species

Unspecified reactive species

Guizela Huelsz-Prince,Rutger Nico Ulbe Kok,Yvonne Goos,Lotte Bruens,Xuan Zheng,Saskia Ellenbroek,Jacco Van Rheenen,Sander Tans,Jeroen S van Zon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (AB175649)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (36)

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