Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) is a preadsorbed secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 90 publications
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- Reproductive biology and endocrinology : RB&E 22:152024Inhibition of mitochondrial cyclophilin D, a downstream target of glycogen synthase kinase 3α, improves sperm motility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeung Hyun Park,Myung Chan GyePubMed 38254112
- The Journal of clinical investigation 133:2023DUSP8 induces TGF-β-stimulated IL-9 transcription and Th9-mediated allergic inflammation by promoting nuclear export of Pur-α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuai-Chia Chuang,Chia-Hsin Hsueh,Pu-Ming Hsu,Ching-Yi Tsai,Ying-Chun Shih,Hsien-Yi Chiu,Yi-Ming Chen,Wen-Kuang Yu,Ming-Han Chen,Tse-Hua TanPubMed 37909329
- The world journal of men's health :2023Regulation of Phosphorylation of Glycogen Synthase Kinase 3α and the Correlation with Sperm Motility in Human.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeung Hyun Park,Young-Pil Kim,Jeong Min Lee,Dong-Wook Park,Ju Tae Seo,Myung Chan GyePubMed 37635337
- Chinese medicine 18:862023Electroacupuncture improves gout arthritis pain via attenuating ROS-mediated NLRP3 inflammasome overactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuina Wei,Boyu Liu,Chengyu Yin,Danyi Zeng,Huimin Nie,Yuanyuan Li,Yan Tai,Xiaofen He,Boyi LiuPubMed 37464384
- The journal of headache and pain 24:612023Neuropeptide Y in the medial habenula alleviates migraine-like behaviors through the Y1 receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunxiao Yang,Zihua Gong,Xiaochen Zhang,Shuai Miao,Bozhi Li,Wei Xie,Tao Wang,Xun Han,Liang Wang,Zhao Dong,Shengyuan YuPubMed 37231359
- Journal of neuroinflammation 20:1092023CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal horn.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Wang,Chengyu Yin,Yushuang Pan,Yunqin Yang,Wei Li,Huadong Ni,Boyu Liu,Huimin Nie,Ruoyao Xu,Huina Wei,Yunwen Zhang,Yuanyuan Li,Qimiao Hu,Yan Tai,Xiaomei Shao,Jianqiao Fang,Boyi LiuPubMed 37158939
- International journal of molecular sciences 24:2023The Voltage-Gated Hv1 H Channel Is Expressed in Tumor-Infiltrating Myeloid-Derived Suppressor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Cozzolino,Adrienn Gyöngyösi,Eva Korpos,Peter Gogolak,Muhammad Umair Naseem,Judit Kállai,Arpad Lanyi,Gyorgy PanyiPubMed 37047188
- Scientific reports 13:41112023Renal toxicity and biokinetics models after repeated uranium instillation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurie De Castro,Annabelle Manoury,Olivier Claude,Bastien Simoneau,Virginie Monceau,David Suhard,Christelle Elie,Victor Magneron,Laurence Roy,Céline Bouvier-Capely,Chrystelle Ibanez,Estelle Davesne,Yann GuéguenPubMed 36914734
- Journal of neurodevelopmental disorders 15:62023NRXN1 depletion in the medial prefrontal cortex induces anxiety-like behaviors and abnormal social phenotypes along with impaired neurite outgrowth in rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Wu,Jiansheng Zhu,Lianghui You,Jingyu Wang,Sufen Zhang,Zhonghui Liu,Qu Xu,Xiaojie Yuan,Lei Yang,Wei Wang,Meiling Tong,Qin Hong,Xia ChiPubMed 36737720
- Science (New York, N.Y.) 379:eabn89342023Low protease activity in B cell follicles promotes retention of intact antigens after immunization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAereas Aung,Ang Cui,Laura Maiorino,Ava P Amini,Justin R Gregory,Maurice Bukenya,Yiming Zhang,Heya Lee,Christopher A Cottrell,Duncan M Morgan,Murillo Silva,Heikyung Suh,Jesse D Kirkpatrick,Parastoo Amlashi,Tanaka Remba,Leah M Froehle,Shuhao Xiao,Wuhbet Abraham,Josetta Adams,J Christopher Love,Phillip Huyett,Douglas S Kwon,Nir Hacohen,William R Schief,Sangeeta N Bhatia,Darrell J IrvinePubMed 36701450
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF, IF
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000.00000 - 1/4000.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application IF | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) is a preadsorbed secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 90 publications
Key facts
- Description
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF, IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Rabbit IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Flow Cytometry - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150061)
Overlay histogram showing Jurkat cells stained with Anti-CD3 epsilon antibody [SP7] ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 epsilon antibody [SP7] ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Donkey anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150061) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150061)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab150061 Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150061)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab150061 Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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