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AB150065

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed

5

(2 Reviews)

|

(49 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 49 publications.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB150065)
  • IHC-P

AbReview73766****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB150065)

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB150065)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB150065)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150065 Alexa Fluor® 448 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB150065)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB150065)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

Flow Cyt, ICC/IF, ELISA, IHC-Fr, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads .
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu4270 PubMed40749060

2025

Neuronal Reg3β/macrophage TNF-α-mediated positive feedback signaling contributes to pain chronicity in a rat model of CRPS-I.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Nie,Boyu Liu,Chengyu Yin,Zishan Dong,Yushuang Pan,Peiyi Li,Qimiao Hu,Jie Wang,Yan Tai,Xiaomei Shao,Guihua Tian,Chuan Wang,Jianqiao Fang,Boyi Liu

PloS one 20:e0326157 PubMed40627604

2025

Deep crypt secretory cells shape region-specific mucin glycosylation patterns in the mouse colon.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Sugahara,Hayato Kawakami,Yoshihiro Akimoto

Molecular therapy. Nucleic acids 36:102499 PubMed40171278

2025

SHMT2 is essential for mammalian preimplantation embryonic development through biosynthesis of nucleotide metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Mingze Shi,Yingxue Huai,Tiantian Deng,Chuanxin Zhang,Jinzhu Song,Jiawei Wang,Yiwen Zhang,Zi-Jiang Chen,Han Zhao,Keliang Wu,Boyang Liu

Scientific reports 15:4216 PubMed39905182

2025

Siponimod supports remyelination in the non-supportive environment.

Applications

Unspecified application

Species

Unspecified reactive species

Johann Krüger,Newshan Behrangi,David Schliep,Leo Heinig,Elise Vankriekelsvenne,Nicole Wigger,Markus Kipp

The EMBO journal 44:1354-1378 PubMed39815005

2025

The microcephaly-associated transcriptional regulator AUTS2 cooperates with Polycomb complex PRC2 to produce upper-layer neurons in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumi Shimaoka,Kei Hori,Satoshi Miyashita,Yukiko U Inoue,Nao K N Tabe,Asami Sakamoto,Ikuko Hasegawa,Kayo Nishitani,Kunihiko Yamashiro,Saki F Egusa,Shoji Tatsumoto,Yasuhiro Go,Manabu Abe,Kenji Sakimura,Takayoshi Inoue,Takuya Imamura,Mikio Hoshino

iScience 27:111246 PubMed39634560

2024

Mesenchymal GDNF promotes intestinal enterochromaffin cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Toni T Lemmetyinen,Emma W Viitala,Linnea Wartiovaara,Pekka Päivinen,Heikki T Virtanen,Nalle Pentinmikko,Pekka Katajisto,Tomi P Mäkelä,Timothy C Wang,Jaan-Olle Andressoo,Saara Ollila

Cellular and molecular gastroenterology and hepatology 18:101405 PubMed39299667

2024

Interindividual Variation in Gut Nitrergic Neuron Density Is Regulated By GDNF Levels and ETV1.

Applications

Unspecified application

Species

Unspecified reactive species

Heikki T Virtanen,Peyman Choopanian,L Lauriina Porokuokka,Richard Forsgård,Daniel R Garton,Soophie Olfat,Riitta Korpela,Mehdi Mirzaie,Jaan-Olle Andressoo

CNS neuroscience & therapeutics 30:e70001 PubMed39154359

2024

Parabrachial nucleus Vglut2 expressing neurons projection to the extended amygdala involved in the regulation of wakefulness during sevoflurane anesthesia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Yan,Yingfu Jiao,Enpeng Liang,Xiaolu Lei,Nan Zhang,Shimao Xu,Lin Zhang,Jiedong Wang,Tianyuan Luo,Jie Yuan,Chengdong Yuan,Hao Yang,Hongxin Dong,Tian Yu,Weifeng Yu

Investigative ophthalmology & visual science 65:7 PubMed38833258

2024

Radiation-Induced DNA Damage in Uveal Melanoma Is Influenced by Dose Delivery and Chromosome 3 Status.

Applications

Unspecified application

Species

Unspecified reactive species

Aysegül Tura,Yingda Zhu,Siranush Vardanyan,Michelle Prasuhn,Vinodh Kakkassery,Julia Lüke,Hartmut Merz,Frank Paulsen,Dirk Rades,Florian Cremers,Karl-Ulrich Bartz-Schmidt,Salvatore Grisanti

Journal of cellular and molecular medicine 28:1-11 PubMed38526036

2024

NEDD4L is a promoter for angiogenesis and cell proliferation in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Binghong Liu,Fei Song,Xiaoxia Zhou,Chan Wu,Huizhu Huang,Weiyin Wu,Gang Li,Yan Wang
View all publications

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