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AB175692

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed

5

(1 Review)

|

(33 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 33 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175692)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175692)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175692, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175692)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175692)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175692, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175692)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (AB175692)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

IHC-P, IHC-Fr, ICC/IF, Flow Cyt, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Rabbit IgG
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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:1354-1378 PubMed39815005

2025

The microcephaly-associated transcriptional regulator AUTS2 cooperates with Polycomb complex PRC2 to produce upper-layer neurons in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumi Shimaoka,Kei Hori,Satoshi Miyashita,Yukiko U Inoue,Nao K N Tabe,Asami Sakamoto,Ikuko Hasegawa,Kayo Nishitani,Kunihiko Yamashiro,Saki F Egusa,Shoji Tatsumoto,Yasuhiro Go,Manabu Abe,Kenji Sakimura,Takayoshi Inoue,Takuya Imamura,Mikio Hoshino

Scientific reports 14:30899 PubMed39730509

2024

Mesenchymal stromal cells reduce inflammation and improve lung function in a mouse model of cystic fibrosis lung disease.

Applications

Unspecified application

Species

Unspecified reactive species

Declan F Doherty,Lydia E Roets,Caoifa M Dougan,Ryan R Brown,Ian J Hawthorne,Cecilia O'Kane,Anna D Krasnodembskaya,Marcus A Mall,Clifford C Taggart,Sinéad Weldon

International journal of molecular sciences 25: PubMed39684875

2024

The Nucleocapsid (N) Proteins of Different Human Coronaviruses Demonstrate a Variable Capacity to Induce the Formation of Cytoplasmic Condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Tikhomirova,Oleg L Kuzmenko,Eugene A Arifulin,Olesya M Shirokova,Yana R Musinova,Eugene V Sheval

Journal of biomedical research 38:500-511 PubMed38808557

2024

RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Xu,Yan Gao,Wenjie Yin,Qinghuai Liu,Songtao Yuan

Viruses 16: PubMed38793552

2024

HIV-1 Capsid Rapidly Induces Long-Lived CPSF6 Puncta in Non-Dividing Cells, but Similar Puncta Already Exist in Uninfected T-Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anabel Guedán,Megan Burley,Eve R Caroe,Kate N Bishop

Biotechnology and bioengineering 121:1435-1452 PubMed38184801

2024

Mechanobiological stimulation in organ-on-a-chip systems reduces hepatic drug metabolic capacity in favor of regenerative specialization.

Applications

Unspecified application

Species

Unspecified reactive species

Benedikt Scheidecker,Stéphane Poulain,Masahiro Sugimoto,Hiroshi Arakawa,Soo H Kim,Takumi Kawanishi,Yukio Kato,Mathieu Danoy,Masaki Nishikawa,Yasuyuki Sakai

eNeuro 10: PubMed36792362

2023

Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Yihe Ma,Wen-Chi Shu,Lin Lin,Xiao-Jie Cao,Donata Oertel,Philip H Smith,Meyer B Jackson

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1668-1681 PubMed36781220

2023

Opioid Withdrawal Abruptly Disrupts Amygdala Circuit Function by Reducing Peptide Actions.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle C Gregoriou,Sahil D Patel,Sebastian Pyne,Bryony L Winters,Elena E Bagley

Frontiers in cellular neuroscience 16:1057887 PubMed36687525

2023

Potential therapeutic mechanism of deep brain stimulation of the nucleus accumbens in obsessive-compulsive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Yifeng Shi,Mengqi Wang,Linglong Xiao,Luolan Gui,Wen Zheng,Lin Bai,Bo Su,Bin Li,Yangyang Xu,Wei Pan,Jie Zhang,Wei Wang

PloS one 17:e0277561 PubMed36355857

2022

Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding.

Applications

Unspecified application

Species

Unspecified reactive species

Kara A Davis,Anuhya Gottipatti,Hsuan Peng,Renee Donahue,Lakshman Chelvarajan,Calvin Cahall,Himi Tripathi,Ahmed Al-Darraji,Shaojing Ye,Ahmed Abdel-Latif,Brad J Berron
View all publications
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