Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 29 publications.
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- Viruses 16:2024HIV-1 Capsid Rapidly Induces Long-Lived CPSF6 Puncta in Non-Dividing Cells, but Similar Puncta Already Exist in Uninfected T-Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnabel Guedán,Megan Burley,Eve R Caroe,Kate N BishopPubMed 38793552
- Biotechnology and bioengineering 121:1435-14522024Mechanobiological stimulation in organ-on-a-chip systems reduces hepatic drug metabolic capacity in favor of regenerative specialization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenedikt Scheidecker,Stéphane Poulain,Masahiro Sugimoto,Hiroshi Arakawa,Soo H Kim,Takumi Kawanishi,Yukio Kato,Mathieu Danoy,Masaki Nishikawa,Yasuyuki SakaiPubMed 38184801
- eNeuro 10:2023Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYihe Ma,Wen-Chi Shu,Lin Lin,Xiao-Jie Cao,Donata Oertel,Philip H Smith,Meyer B JacksonPubMed 36792362
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1668-16812023Opioid Withdrawal Abruptly Disrupts Amygdala Circuit Function by Reducing Peptide Actions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabrielle C Gregoriou,Sahil D Patel,Sebastian Pyne,Bryony L Winters,Elena E BagleyPubMed 36781220
- Frontiers in cellular neuroscience 16:10578872023Potential therapeutic mechanism of deep brain stimulation of the nucleus accumbens in obsessive-compulsive disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifeng Shi,Mengqi Wang,Linglong Xiao,Luolan Gui,Wen Zheng,Lin Bai,Bo Su,Bin Li,Yangyang Xu,Wei Pan,Jie Zhang,Wei WangPubMed 36687525
- PloS one 17:e02775612022Gelatin coating enhances therapeutic cell adhesion to the infarcted myocardium via ECM binding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKara A Davis,Anuhya Gottipatti,Hsuan Peng,Renee Donahue,Lakshman Chelvarajan,Calvin Cahall,Himi Tripathi,Ahmed Al-Darraji,Shaojing Ye,Ahmed Abdel-Latif,Brad J BerronPubMed 36355857
- PLoS pathogens 18:e10103492022SARS-CoV-2 ORF6 disrupts innate immune signalling by inhibiting cellular mRNA export.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoss Hall,Anabel Guedán,Melvyn W Yap,George R Young,Ruth Harvey,Jonathan P Stoye,Kate N BishopPubMed 36007063
- Alzheimer's & dementia (New York, N. Y.) 8:e123342022Dysfunction of the retromer complex system contributes to amyloid and tau pathology in a stem cell model of Down syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMary Elizabeth Curtis,Tiffany Smith,Benjamin E Blass,Domenico PraticòPubMed 35910668
- Journal of medical genetics :2022Novel mutations identified in patients with IMAGE-I syndrome cause aberrant subcellular localisation and protein degradation in the nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomohiro Nakano,Yoji Sasahara,Atsuo Kikuchi,Kunihiko Moriya,Hidetaka Niizuma,Tetsuya Niihori,Matsuyuki Shirota,Ryo Funayama,Keiko Nakayama,Yoko Aoki,Shigeo KurePubMed 35534205
- Reproductive biology and endocrinology : RB&E 20:722022Increased testicular insulin-like growth factor 1 is associated with gonadal activation by recombinant growth hormone in immature rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Xu,Chang Yong Han,Mi Jung Park,Myung Chan GyePubMed 35459135
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 29 publications.
Key facts
- Description
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175692, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® 568 is pseudocolored in yellow.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175692, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® 568 is pseudocolored in yellow.
Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
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