Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Cited in 153 publications.
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- Cell biochemistry and function 42:e40052024Icariin alleviates renal inflammation and tubulointerstitial fibrosis via Nrf2-mediated attenuation of mitochondrial damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Ding,Shanyue Sun,Shuting Zhou,Zhimei Lv,Rong WangPubMed 38583082
- Neurotrauma reports 5:61-732024Lifelong Chronic Sleep Disruption in a Mouse Model of Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew R Morris,Erwin K Gudenschwager Basso,Miguel A Gutierrez-Monreal,Rawad Daniel Arja,Firas H Kobeissy,Christopher G Janus,Kevin K W Wang,Jiepei Zhu,Andrew C LiuPubMed 38288298
- International journal of molecular sciences 25:2024The Abundant Distribution and Duplication of SARS-CoV-2 in the Cerebrum and Lungs Promote a High Mortality Rate in Transgenic hACE2-C57 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Li,Xin Zhao,Shasha Peng,Yingyan Li,Jing Li,Huiwen Zheng,Yifan Zhang,Yurong Zhao,Yuan Tian,Jinling Yang,Yibin Wang,Xinglong Zhang,Longding LiuPubMed 38256071
- iScience 27:1086702023Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Hennegan,Aled H Bryant,Lauren Griffiths,Matthieu Trigano,Oliver J M Bartley,Joanna J Bartlett,Carys Minahan,Willy Antoni Abreu de Oliveira,Eylan Yutuc,Sotirios Ntikas,Christos S Bartsocas,Margarita Markouri,Eleni Antoniadou,Ioanna Laina,Owain W Howell,Meng Li,Yuqin Wang,William J Griffiths,Emma L Lane,Mariah J Lelos,Spyridon TheofilopoulosPubMed 38155767
- Cell death discovery 9:3682023GSDMD promotes neutrophil extracellular traps via mtDNA-cGAS-STING pathway during lung ischemia/reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Zhao,Fangte Liang,Mengling Ye,Siyi Wu,Yi Qin,Lu Zhao,Lu Zhang,Jing He,Liming Cen,Fei LinPubMed 37794018
- Brain circulation 9:162-1712023Glycogen synthase kinase-3β mediates toll-like receptors 4/nuclear factor kappa-B-activated cerebral ischemia-reperfusion injury through regulation of fat mass and obesity-associated protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiwei Xu,Wenwen Du,Xiuxiu Zhuang,Dongdong Liang,Yunchang Mo,Junlu WangPubMed 38020949
- Investigative ophthalmology & visual science 64:262023Norepinephrine as the Intrinsic Contributor to Contact Lens-Induced Pseudomonas aeruginosa Keratitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBi Ning Zhang,Benxiang Qi,Wai Kit Chu,Fangying Song,Suxia Li,Qiaoqiao Dong,Zheng Shao,Bin Zhang,Xianli Du,Xiubin Ma,Vishal Jhanji,Qingjun ZhouPubMed 37234000
- iScience 26:1074962023Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Liu,Lu Zhang,Zhen Liu,Jinyuan Lin,Xiaojing He,Siyi Wu,Yi Qin,Chen Zhao,Youyuan Guo,Fei LinPubMed 37636061
- Journal of neurochemistry 166:747-7622023JAG-1/Notch signaling axis in the spinal cord contributes to bone cancer pain in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Fu,Baoxia Zhao,Ge Luo,Huadong Ni,Longsheng Xu,Qiuli He,Miao Xu,Chengfei Xu,Yahui Wang,Chaobo Ni,Ming YaoPubMed 37422446
- Scientific reports 13:85532023Deep ultraviolet fluorescence microscopy of three-dimensional structures in the mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepa Kamath Kasaragod,Hidenori AizawaPubMed 37237102
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Cited in 153 publications.
Key facts
- Description
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594)
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
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Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150076 Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
Cross-reactivity of the polyclonal secondary antibody Donkey Anti-Rabbit IgG H&L ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Donkey Anti-Rabbit IgG H&L ab182020 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (Goat Anti-Donkey IgG H&L (HRP) ab6988) was used at 1/20,000 dilution (50 μl/well), followed by incubation for 1h at RT.
For the batch tested, Donkey Anti-Rabbit IgG H&L ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.
This data was developed using the unconjugated antibody (Donkey Anti-Rabbit IgG H&L ab182020).
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