Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed
5
(1 Review)
|
(102 Publications)
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 70 publications
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150064)
ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150064 Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150064)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150064 Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150064)
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Reactivity data
Product details
This antibody reacts with the heavy and light chains of Rabbit IgG
Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
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Shipped at conditions
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Aliquoting information
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Target data
Publications (102)
Recent publications for all applications. Explore the full list and refine your search
Nature cardiovascular research 4:1466-1486 PubMed41053445
2025
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Nucleic acids research 53: PubMed41002028
2025
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Science advances 11:eadz1228 PubMed40929275
2025
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Translational oncology 61:102495 PubMed40803028
2025
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Science advances 11:eadu4270 PubMed40749060
2025
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Scientific reports 15:20457 PubMed40596183
2025
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Investigative ophthalmology & visual science 66:42 PubMed40434346
2025
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Molecular therapy. Nucleic acids 36:102499 PubMed40171278
2025
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Communications biology 8:466 PubMed40119106
2025
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413253 PubMed40087815
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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