AB150068

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

Name: Donkey Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 594
Brand: Abcam Limited
SKU: AB150068

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(17 Publications)

Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 17 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150068, donkey polyclonal secondary antibody to Rabbit IgG - H&L (Alexa Fluor®; 594), pre-adsorbed, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150068, donkey polyclonal secondary antibody to Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Chicken, Cow, Human, Pig, Goat

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, ICC/IF, IHC-Fr, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed41002428

2025

Expansion Microscopy of the Enteric Nervous System: A Feasibility Study.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Wenchuan Zhang,Menachem Hanani

Bioengineering (Basel, Switzerland) 11: PubMed39199753

2024

Formation and Long-Term Culture of hiPSC-Derived Sensory Nerve Organoids Using Microfluidic Devices.

Applications

Unspecified application

Species

Unspecified reactive species

Takuma Ogawa,Souichi Yamada,Shuetsu Fukushi,Yuya Imai,Jiro Kawada,Kazutaka Ikeda,Seii Ohka,Shohei Kaneda

JCI insight 9: PubMed38888971

2024

A dual-acting DNASE1/DNASE1L3 biologic prevents autoimmunity and death in genetic and induced lupus models.

Applications

Unspecified application

Species

Unspecified reactive species

Paul R Stabach,Dominique Sims,Eduardo Gomez-Bañuelos,Sandra Zehentmeier,Kris Dammen-Brower,Andrew Bernhisel,Sophia Kujawski,Sam G Lopez,Michelle Petri,Daniel W Goldman,Ethan R Lester,Quan Le,Tayyaba Ishaq,Hana Kim,Shivani Srivastava,Deepika Kumar,Joao P Pereira,Kevin J Yarema,Fotios Koumpouras,Felipe Andrade,Demetrios T Braddock

Science advances 9:eadf1332 PubMed37878712

2023

Human cerebrospinal fluid affects chemoradiotherapy sensitivities in tumor cells from patients with glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Brett W Stringer,Manam Inushi De Silva,Zarina Greenberg,Alejandra Noreña Puerta,Robert Adams,Bridget Milky,Michael Zabolocki,Mark van den Hurk,Lisa M Ebert,Christine Fairly Bishop,Simon J Conn,Ganessan Kichenadasse,Michael Z Michael,Rebecca J Ormsby,Santosh Poonoose,Cedric Bardy

Frontiers in neuroscience 17:1142785 PubMed37056311

2023

Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yukie Shirakawa,Sachi N Ohno,Kanae A Yamagata,Eriko Kuramoto,Yoshiaki Oda,Takahiro J Nakamura,Wataru Nakamura,Mitsutaka Sugimura

The Journal of cell biology 222: PubMed36383135

2022

Mitochondrial dysfunction compromises ciliary homeostasis in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Olesia Ignatenko,Satu Malinen,Sofiia Rybas,Helena Vihinen,Joni Nikkanen,Aleksander Kononov,Eija S Jokitalo,Gulayse Ince-Dunn,Anu Suomalainen

Frontiers in aging neuroscience 14:935033 PubMed35983379

2022

Development of a system to analyze oral frailty associated with Alzheimer's disease using a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Eriko Kuramoto,Ayano Kitawaki,Takakazu Yagi,Hiroshi Kono,Shin-Ei Matsumoto,Hiromitsu Hara,Yasumasa Ohyagi,Haruki Iwai,Atsushi Yamanaka,Tetsuya Goto

Neurotoxicity research 40:775-790 PubMed35471722

2022

Isoflurane and Sevoflurane Induce Cognitive Impairment in Neonatal Rats by Inhibiting Neural Stem Cell Development Through Microglial Activation, Neuroinflammation, and Suppression of VEGFR2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chunlong Zuo,Junmei Ma,Yizhao Pan,Dongxu Zheng,Chunjiang Chen,Naqi Ruan,Ying Su,Haihan Nan,Qingquan Lian,Han Lin

International journal of molecular sciences 22: PubMed34502042

2021

Loose Morphology and High Dynamism of OSER Structures Induced by the Membrane Domain of HMG-CoA Reductase.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Enrique Grados-Torrez,Carmen López-Iglesias,Joan Carles Ferrer,Narciso Campos

Frontiers in pharmacology 11:533809 PubMed33071778

2020

Morroniside Inhibits HO-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy .

Applications

Unspecified application

Species

Unspecified reactive species

Xue Gao,Yi Liu,Lin Wang,Na Sai,Yixiu Liu,Jian Ni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150068)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (17)

Product promise