Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 43 publications.
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- Stem cell research & therapy 14:3682023New characterization and safety evaluation of human limbal stem cells used in clinical application: fidelity of mitotic process and mitotic spindle morphologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarija Zekušić,Marina Bujić Mihica,Marija Skoko,Kruno Vukušić,Patrik Risteski,Jelena Martinčić,Iva M Tolić,Krešo Bendelja,Snježana Ramić,Tamara Dolenec,Ivana Vrgoč Zimić,Dominik Puljić,Ivanka Petric Vicković,Renata Iveković,Ivanka Batarilo,Uršula Prosenc Zmrzljak,Alan Hoffmeister,Tiha VučemiloPubMed 38093301
- Cell 186:5910-5924.e172023A tridimensional atlas of the developing human head.Applications:IHC - WmtReactive species:HumanRaphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain ChédotalPubMed 38070509
- The Journal of physiology 601:5751-57752023M-type potassium currents differentially affect activation of motoneuron subtypes and tune recruitment gain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon A Sharples,Matthew J Broadhead,James A Gray,Gareth B MilesPubMed 37988235
- Burns & trauma 11:tkad0232023The m6A reader YTHDF2 alleviates the inflammatory response by inhibiting IL-6R/JAK2/STAT1 pathway-mediated high-mobility group box-1 release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuo Zeng,Yingying Lan,Lijuan Zhang,Yu Chen,Yali Gong,Fangqing Zuo,Junda Li,Gaoxing Luo,Yizhi Peng,Zhiqiang YuanPubMed 38026444
- Particle and fibre toxicology 20:342023Amorphous silica nanoparticles cause abnormal cytokinesis and multinucleation through dysfunction of the centralspindlin complex and microfilaments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyan Xiao,Jinyan Pang,Hua Qin,Liyang Dou,Man Yang,Ji Wang,Xianqing Zhou,Yang Li,Junchao Duan,Zhiwei SunPubMed 37608338
- International journal of biological sciences 19:3428-34402023Lats2 deficiency protects the heart against myocardial infarction by reducing inflammation and inhibiting mitochondrial fission and STING/p65 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLibao Liu,Shuai Huang,Yingzhen Du,Hao Zhou,Kai Zhang,Jinyuan HePubMed 37497006
- iScience 26:1069852023Dissection of insular cortex layer 5 reveals two sublayers with opposing modulatory roles in appetitive drinking behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Takemoto,Shigeki Kato,Kazuto Kobayashi,Wen-Jie SongPubMed 37378339
- Biology of sex differences 14:82023Investigation of autism-related transcription factors underlying sex differences in the effects of bisphenol A on transcriptome profiles and synaptogenesis in the offspring hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSurangrat Thongkorn,Songphon Kanlayaprasit,Kasidit Kasitipradit,Pattanachat Lertpeerapan,Pawinee Panjabud,Valerie W Hu,Depicha Jindatip,Tewarit SarachanaPubMed 36803626
- Science (New York, N.Y.) 379:eabn89342023Low protease activity in B cell follicles promotes retention of intact antigens after immunization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAereas Aung,Ang Cui,Laura Maiorino,Ava P Amini,Justin R Gregory,Maurice Bukenya,Yiming Zhang,Heya Lee,Christopher A Cottrell,Duncan M Morgan,Murillo Silva,Heikyung Suh,Jesse D Kirkpatrick,Parastoo Amlashi,Tanaka Remba,Leah M Froehle,Shuhao Xiao,Wuhbet Abraham,Josetta Adams,J Christopher Love,Phillip Huyett,Douglas S Kwon,Nir Hacohen,William R Schief,Sangeeta N Bhatia,Darrell J IrvinePubMed 36701450
- Journal of tissue engineering 14:204173142211492072023Estradiol-17β [E] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Shafaat,Sabiniano Roman Regueros,Christopher Chapple,Sheila MacNeil,Vanessa HearndenPubMed 36726532
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/1000.00000 - 1/2000.00000 | Notes ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody. |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
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Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 43 publications.
Key facts
- Description
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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4 product images
Flow Cytometry - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150063)
Overlay histogram showing Jurkat cells stained with Anti-CD3 epsilon antibody [SP7] ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 epsilon antibody [SP7] ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Donkey anti-rabbit IgG H&L (Alexa Fluor® 647) (ab150063) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150063)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150063 Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150063)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150063 Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150063)
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