JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB150067

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

5

(1 Review)

|

(32 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 32 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150067)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150067)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150067 Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150067)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB150067)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p><a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.</p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40075680

2025

Cystatin M/E Ameliorates Multiple Myeloma-Induced Hyper Osteolytic Bone Resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Dongzheng Gai,Perry C Caviness,Oxana P Lazarenko,Jennifer F Chen,Christopher E Randolph,Zijun Zhang,Yan Cheng,Fumou Sun,Hongwei Xu,Michael L Blackburn,Guido Tricot,John D Shaughnessy,Jin-Ran Chen,Fenghuang Zhan

eLife 14: PubMed39882846

2025

A hierarchical pathway for assembly of the distal appendages that organize primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoharu Kanie,Beibei Liu,Julia F Love,Saxton D Fisher,Anna-Karin Gustavsson,Peter K Jackson

Science advances 11:eadq0651 PubMed39823324

2025

Activation of locus coeruleus noradrenergic neurons rapidly drives homeostatic sleep pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Silverman,Changwan Chen,Shuang Chang,Lillie Bui,Yufan Zhang,Rishi Raghavan,Anna Jiang,April Le,Dana Darmohray,Jiao Sima,Xinlu Ding,Bing Li,Chenyan Ma,Yang Dan

The EMBO journal 44:1354-1378 PubMed39815005

2025

The microcephaly-associated transcriptional regulator AUTS2 cooperates with Polycomb complex PRC2 to produce upper-layer neurons in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumi Shimaoka,Kei Hori,Satoshi Miyashita,Yukiko U Inoue,Nao K N Tabe,Asami Sakamoto,Ikuko Hasegawa,Kayo Nishitani,Kunihiko Yamashiro,Saki F Egusa,Shoji Tatsumoto,Yasuhiro Go,Manabu Abe,Kenji Sakimura,Takayoshi Inoue,Takuya Imamura,Mikio Hoshino

Fluids and barriers of the CNS 22:4 PubMed39789614

2025

Plasma S100β is a predictor for pathology and cognitive decline in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Geetika Nehra,Bryan J Maloney,Rebecca R Smith,Wijitra Chumboatong,Erin L Abner,Peter T Nelson,Björn Bauer,Anika M S Hartz

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2310048 PubMed39474995

2024

Cathepsin K-Positive Cell Lineage Promotes In Situ Dentin Formation Controlled by Nociceptive Sonic Hedgehog.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoshi Xu,Xiaohan Zhang,Weimin Lin,Yushun Wang,Danting Zhang,Shuang Jiang,Linfeng Liu,Jiaying Wang,Xutao Luo,Xiao Zhang,Junjun Jing,Quan Yuan,Chenchen Zhou

Bioactive materials 43:82-97 PubMed39345992

2024

Ultrasound-generated bubbles enhance osteogenic differentiation of mesenchymal stromal cells in composite collagen hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Somnath Maji,Mitra Aliabouzar,Carole Quesada,Anjali Chiravuri,Aidan Macpherson,Abigail Pinch,Karsyn Kazyak,Ziyad Emara,Bachir A Abeid,Robert N Kent,Firaol S Midekssa,Man Zhang,Brendon M Baker,Renny T Franceschi,Mario L Fabiilli

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303291 PubMed37553778

2023

Lepr-Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1.

Applications

Unspecified application

Species

Unspecified reactive species

Danting Zhang,Weimin Lin,Shuang Jiang,Peng Deng,Linfeng Liu,Qian Wang,Rui Sheng,Hui Sophie Shu,Lijun Wang,Weiguo Zou,Bo O Zhou,Junjun Jing,Ling Ye,Bo Yu,Shiwen Zhang,Quan Yuan

Frontiers in immunology 14:1142492 PubMed36969163

2023

Human intestinal epithelial cells can internalize luminal fungi LC3-associated phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarit Cohen-Kedar,Efrat Shaham Barda,Keren Masha Rabinowitz,Danielle Keizer,Hanan Abu-Taha,Shoshana Schwartz,Kawsar Kaboub,Liran Baram,Eran Sadot,Ian White,Nir Wasserberg,Meirav Wolff-Bar,Adva Levy-Barda,Iris Dotan

Veterinarni medicina 68:33-37 PubMed38384992

2023

Apocrine cystomatosis: From the aspect of epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Tae-Un Kim,Seoung-Woo Lee,Su-Min Baek,Jae-Hyuk Yim,Young-Jin Lee,Jun-Hyeok Son,Sang-Joon Park,Jin-Kyu Park
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com