Suitable for WB. Cited in 18 publications.
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- Endocrine connections 12:2023Cyclic AMP mediates ovine cumulus-oocyte gap junctional function via balancing connexin 43 expression and phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufen Zhao,Erge Namei,Bingxue Yang,Xiangnan Bao,Wei Sun,Gerile Subudeng,Guifang Cao,Haijun Li,Gui WangPubMed 37855365
- Endocrine connections :2023Cyclic AMP mediates ovine cumulus-oocyte gap junctional function via balancing connexin43 expression and phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufen Zhao,Erge Namei,Bingxue Yang,Xiangnan Bao,Wei Sun,Gerile Subudeng,Guifang Cao,Haijun Li,Gui WangPubMed 37721218
- Scientific reports 13:102882023Hyperphagia of female UCP1-deficient mice blunts anti-obesity effects of FGF21.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlou Klein Hazebroek,Rutger Laterveer,Maria Kutschke,Vida Ramšak Marčeta,Clarissa S Barthem,Susanne KeipertPubMed 37355753
- Virologica Sinica 38:128-1412022Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Chen,Jian Liu,Zhilu Chen,Daobin Feng,Cuisong Zhu,Jun Fan,Shuye Zhang,Xiaoyan Zhang,Jianqing XuPubMed 36509386
- Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine 42:463-4752022Low-Intensity Focused Ultrasound Ameliorates Ischemic Heart Failure Related to the Cholinergic Anti-Inflammatory Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenrong Zhong,Qianyun Cai,Longhe Zhong,Yuegang Wang,Fengchu Liang,Zhe Deng,Shasha Li,Daogang Zha,Weibao Qiu,Juefei WuPubMed 36444908
- mSphere 7:e00211222022Angiotensin-Converting Enzyme 2 Potentiates SARS-CoV-2 Infection by Antagonizing Type I Interferon Induction and Its Down-Stream Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Chen,Jian Liu,Zhilu Chen,Haoran Peng,Cuisong Zhu,Daobin Feng,Shuye Zhang,Ping Zhao,Xiaoyan Zhang,Jianqing XuPubMed 35862802
- Oncology letters 24:2672022Lysophosphatidic acid protects cervical cancer HeLa cells from apoptosis induced by doxorubicin hydrochloride.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXibo Wang,Haihua Wang,Xiaoxiao Mou,Yilin Xu,Wenbo Han,Aimin Huang,Yanwei Li,Hui Jiang,Xiaoyun Yang,Zhenbo HuPubMed 35782896
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Chen,Jun Fan,Zhilu Chen,Miaomiao Zhang,Haoran Peng,Jian Liu,Longfei Ding,Mingbin Liu,Chen Zhao,Ping Zhao,Shuye Zhang,Xiaoyan Zhang,Jianqing XuPubMed 34873039
- Frontiers in pharmacology 12:7448262021Anlotinib Inhibits PFKFB3-Driven Glycolysis in Myofibroblasts to Reverse Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeimou Chen,Jinming Zhang,Wenshan Zhong,Yuanyuan Liu,Ye Lu,Zhaojin Zeng,Haohua Huang,Xuan Wan,Xiaojing Meng,Fei Zou,Shaoxi Cai,Hangming DongPubMed 34603058
- Reproduction (Cambridge, England) 160:853-8622020The cAMP-ERK1/2 signaling pathway regulates urokinase-type plasminogen activator-induced bovine granulosa cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufen Zhao,Boyang Yu,Xinyu Liu,Jitu Hu,Yanyan Yang,Erge Namei,Bingxue Yang,Yue Bai,Yinghong Qian,Haijun LiPubMed 33112787
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 680
- Excitation/Emission
- Ex: 679nm, Em: 702nm
- Applications
- WB
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Version
3 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for WB. Cited in 18 publications.
Key facts
- Description
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680)
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 680
- Excitation/Emission
- Ex: 679nm, Em: 702nm
- Applications
- WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680), ab175772 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680), ab175772 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-alpha Tubulin antibody - Loading Control ab18251 overnight at 4°C. Antibody binding was detected using ab175772 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody - Loading Control ab18251) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 5 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 2 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 1 µg
SecondaryAll lanes: Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa
This image is courtesy of a customer review submitted by Christian MarxWestern blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
Blocking: 1 hour at room temperature with 5% Milk in TBST.
Diluent: 2% Milk in TBST
13% SDS-PAGE gel used.
Secondary antibody - donkey anti-rabbit Alexa Fluor 680 (ab175772)
All lanes: Western blot - Anti-p53 (phospho S6) antibody [Y179] (Anti-p53 (phospho S6) antibody [Y179] ab32132) at 1/500 dilution
Lane 1: DMSO treated HCT116 cell line at 20 µg
Lane 2: 10 µM irinotecan (CPT-11) treated HCT116 cell line for 24 hours at 20 µg
SecondaryAll lanes: Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772) at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Exposure time: 3min
ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
Cross-reactivity of the polyclonal secondary antibody Donkey Anti-Rabbit IgG H&L ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Donkey Anti-Rabbit IgG H&L ab182020 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (Goat Anti-Donkey IgG H&L (HRP) ab6988) was used at 1/20,000 dilution (50 μl/well), followed by incubation for 1h at RT.
For the batch tested, Donkey Anti-Rabbit IgG H&L ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.
This data was developed using the unconjugated antibody (Donkey Anti-Rabbit IgG H&L ab182020).
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