Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed
5
(1 Review)
|
(7 Publications)
Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.
View Alternative Names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
- WB
Lab
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (AB186692)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab186692 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes:
Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL
All lanes:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186692) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
false
Reactivity data
Product details
We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed, ab186692 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed, ab186692 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Target data
Publications (7)
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Redox biology 81:103560 PubMed39986117
2025
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Unspecified reactive species
Cancer & metabolism 12:34 PubMed39538356
2024
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Unspecified reactive species
Clinical science (London, England : 1979) 138:173-187 PubMed38315575
2024
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Unspecified reactive species
Journal of medicinal chemistry 63:2511-2526 PubMed32017849
2020
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Unspecified reactive species
Cell reports 26:1598-1613.e8 PubMed30726741
2019
Applications
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Unspecified reactive species
Journal of anatomy 232:965-978 PubMed29473159
2018
Applications
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Unspecified reactive species
Scientific reports 6:34635 PubMed27698380
2016
Applications
Unspecified application
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Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com