Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed
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(6 Publications)
- WB
Lab
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175728)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175728 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes:
Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL
All lanes:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175728) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa,50 kDa
false
Reactivity data
Product details
We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175728 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175728 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
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Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Target data
Publications (6)
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Cell reports. Medicine 5:101532 PubMed38670097
2024
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Communications biology 6:1183 PubMed37985874
2023
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Nanomaterials (Basel, Switzerland) 13: PubMed36678083
2023
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Frontiers in molecular neuroscience 14:681868 PubMed34248499
2021
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The European journal of neuroscience 50:3968-3984 PubMed31215698
2019
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Cell reports 26:1598-1613.e8 PubMed30726741
2019
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com