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AB175728

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed

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(6 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
1 Images
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175728)
  • WB

Lab

Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (AB175728)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175728 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175728) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 37 kDa,50 kDa

false

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175728 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175728 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101532 PubMed38670097

2024

Targeting PDGF signaling of cancer-associated fibroblasts blocks feedback activation of HIF-1α and tumor progression of clear cell ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaro Mori,Yoshie Okimoto,Hiroaki Sakai,Yusuke Kanda,Hirokazu Ohata,Daisuke Shiokawa,Mikiko Suzuki,Hiroshi Yoshida,Haruka Ueda,Tomoyuki Sekizuka,Ryo Tamura,Kaoru Yamawaki,Tatsuya Ishiguro,Raul Nicolas Mateos,Yuichi Shiraishi,Yasushi Yatabe,Akinobu Hamada,Kosuke Yoshihara,Takayuki Enomoto,Koji Okamoto

Communications biology 6:1183 PubMed37985874

2023

Elevated stress response marks deeply quiescent reserve cells of gastric chief cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Shiokawa,Hiroaki Sakai,Miho Koizumi,Yoshie Okimoto,Yutaro Mori,Yusuke Kanda,Hirokazu Ohata,Hiroaki Honda,Koji Okamoto

Nanomaterials (Basel, Switzerland) 13: PubMed36678083

2023

In Vitro Analysis of Superparamagnetic Iron Oxide Nanoparticles Coated with APTES as Possible Radiosensitizers for HNSCC Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Emer,Laura S Hildebrand,Bernhard Friedrich,Rainer Tietze,Rainer Fietkau,Luitpold V Distel

Frontiers in molecular neuroscience 14:681868 PubMed34248499

2021

Evaluation of Neuropathological Features in the SOD1-G93A Low Copy Number Transgenic Mouse Model of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Molnar-Kasza,Barbara Hinteregger,Joerg Neddens,Roland Rabl,Stefanie Flunkert,Birgit Hutter-Paier

The European journal of neuroscience 50:3968-3984 PubMed31215698

2019

The Ventral Tegmental Area has calbindin neurons with the capability to co-release glutamate and dopamine into the nucleus accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

Smriti Mongia,Tsuyoshi Yamaguchi,Bing Liu,Shiliang Zhang,Huiling Wang,Marisela Morales

Cell reports 26:1598-1613.e8 PubMed30726741

2019

Flavivirus NS1 Triggers Tissue-Specific Vascular Endothelial Dysfunction Reflecting Disease Tropism.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Puerta-Guardo,Dustin R Glasner,Diego A Espinosa,Scott B Biering,Mark Patana,Kalani Ratnasiri,Chunling Wang,P Robert Beatty,Eva Harris
View all publications

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