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AB186693

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed

5

(1 Review)

|

(4 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
1 Images
Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (AB186693)
  • WB

Lab

Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (AB186693)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab186693 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL

All lanes:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed (ab186693) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 790

Excitation/Emission

Ex: 782nm, Em: 805nm

Applications

WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed, ab186693 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed, ab186693 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:5910-5924.e17 PubMed38070509

2023

A tridimensional atlas of the developing human head.

Applications

IHC - Wmt

Species

Human

Raphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain Chédotal

Frontiers in cardiovascular medicine 8:748891 PubMed34988124

2021

The Role of Sorting Nexin 17 in Cardiac Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yufei Wu,Yaqun Zhou,Jian Huang,Ke Ma,Tianyou Yuan,Yong Jiang,Maoqing Ye,Jun Li

Human genetics 139:1499-1511 PubMed32533362

2020

A novel variant in GPAA1, encoding a GPI transamidase complex protein, causes inherited vascular anomalies with various phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyun Li,Liu Yang,Jie Yang,Jiahao Shi,Peiwei Chai,Shengfang Ge,Yefei Wang,Xianqun Fan,Renbing Jia

Scientific reports 9:15901 PubMed31685846

2019

Metabolic Activation of Flavin Monooxygenase-mediated Trimethylamine-N-Oxide Formation in Experimental Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Prokopienko,Raymond E West,Daniel P Schrum,Jason R Stubbs,François A Leblond,Vincent Pichette,Thomas D Nolin
View all publications

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