AB97061

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)

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(11 Publications)

Suitable for ICC, ELISA, IHC-P, WB. Cited in 11 publications.

1 Images

Unknown

Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB97061)

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-loading-control-ab8227'>ab8227</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution

Lanes 3 - 4:

Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution

Lanes 5 - 6:

Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution

Predicted band size: 36 kDa

true

Exposure time: 3min

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

WB, IHC-P, ICC, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"" } } }

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Product details

Part of the AbExcel range.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 36:ar2 PubMed39565680

2024

The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany Friedson,Stephen D Willis,Natalia Shcherbik,Alicia N Campbell,Katrina F Cooper

PloS one 18:e0289745 PubMed37556495

2023

Analytical sameness methodology for the evaluation of structural, physicochemical, and biological characteristics of Armlupeg: A pegfilgrastim biosimilar case study.

Applications

Unspecified application

Species

Unspecified reactive species

Arati Deshmukh,Rishank Goyal,Kalyana Sundaram,Kaustubh Dange,Tejshri Lakhote,Sanjay Niranjan,Jennifer Bharucha,Ashok Mishra,Bhavesh Vats,Sanjay Tiwari

Frontiers in cell and developmental biology 10:867257 PubMed35433688

2022

Cyclin C-Cdk8 Kinase Phosphorylation of Rim15 Prevents the Aberrant Activation of Stress Response Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen D Willis,Sara E Hanley,Steven J Doyle,Katherine Beluch,Randy Strich,Katrina F Cooper

iScience 25:103823 PubMed35198885

2022

Aberrant cyclin C nuclear release induces mitochondrial fragmentation and dysfunction in syndrome fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Ti Chang,Jan Jezek,Alicia N Campbell,David C Stieg,Zachary A Kiss,Kevin Kemper,Ping Jiang,Hyung-Ok Lee,Warren D Kruger,Peter M van Hasselt,Randy Strich

Autophagy 17:3547-3565 PubMed33678121

2021

Snx4-assisted vacuolar targeting of transcription factors defines a new autophagy pathway for controlling expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sara E Hanley,Stephen D Willis,Katrina F Cooper

Communications biology 4:106 PubMed33495563

2021

Porphyromonas gingivalis fimbrial protein Mfa5 contains a von Willebrand factor domain and an intramolecular isopeptide.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas V Heidler,Karin Ernits,Agnieszka Ziolkowska,Rolf Claesson,Karina Persson

Journal of cell science 132: PubMed31331961

2019

Synergistic repression of thyroid hyperplasia by cyclin C and Pten.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Jezek,Kun Wang,Ruilan Yan,Antonio Di Cristofano,Katrina F Cooper,Randy Strich

EMBO reports 20:e47425 PubMed31385392

2019

Mitochondrial translocation of cyclin C stimulates intrinsic apoptosis through Bax recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Jezek,Kai-Ti Chang,Amogh M Joshi,Randy Strich

Carcinogenesis 38:847-858 PubMed28535183

2017

Time-caloric restriction inhibits the neoplastic transformation of cirrhotic liver in rats treated with diethylnitrosamine.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Molina-Aguilar,María de Jesús Guerrero-Carrillo,Jesús Javier Espinosa-Aguirre,Sitlali Olguin-Reyes,Thania Castro-Belio,Olivia Vázquez-Martínez,Julieta Berenice Rivera-Zavala,Mauricio Díaz-Muñoz

Molecular and cellular neurosciences 74:58-70 PubMed26947098

2016

Lysine-specific demethylase-1 (LSD1) is compartmentalized at nuclear chromocenters in early post-mitotic cells of the olfactory sensory neuronal lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Seda Kilinc,Alyssa Savarino,Julie H Coleman,James E Schwob,Robert P Lane

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)

Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (AB97061)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (11)

Product promise