Suitable for ICC, ELISA, IHC-P, WB. Cited in 10 publications.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/5000.00000 - 1/50000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/5000.00000 - 1/50000.00000 | Notes - |
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Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Suitable for ICC, ELISA, IHC-P, WB. Cited in 10 publications.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
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All lanes: Western blot - Anti-beta Actin antibody (Anti-beta Actin antibody ab8227) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 1 - 2: Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution
Lanes 3 - 4: Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution
Lanes 5 - 6: Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 3min
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