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Suitable for Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

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Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Conjugation

Alkaline Phosphatase

Applications

Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB

Clonality

Polyclonal

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Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/400 - 1/2000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/4000 - 1/16000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

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Suitable for Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

Key facts

Description

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Conjugation

Alkaline Phosphatase

Applications

Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB

Clonality

Polyclonal

Pre-adsorbed

Yes

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Concentration
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Properties

Form

Liquid

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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