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AB7084

Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed

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(5 Publications)

Suitable for Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alkaline Phosphatase

Excitation/Emission
Applications

ICC/IF, IHC-P, WB, IHC-Fr, Dot, ELISA

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/400 - 1/2000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/4000 - 1/16000", "notes":"<p></p>" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 8 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:5674 PubMed39955442

2025

Extracellular vesicles efficiently deliver survival motor neuron protein to cells in culture.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte A René,Robin J Parks

Microbiology spectrum 10:e0307522 PubMed36200898

2022

Superinfection with SARS-CoV-2 Has Deleterious Effects on Mycobacterium bovis BCG Immunity and Promotes Dissemination of Mycobacterium tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel E Hildebrand,Shaswath Sekar Chandrasekar,Mariah Riel,Bubacarr J B Touray,Sophie A Aschenbroich,Adel M Talaat

Cancers 13: PubMed34203855

2021

The RAD51-FFPE Test; Calibration of a Functional Homologous Recombination Deficiency Test on Diagnostic Endometrial and Ovarian Tumor Blocks.

Applications

Unspecified application

Species

Unspecified reactive species

Lise M van Wijk,Claire J H Kramer,Sylvia Vermeulen,Natalja T Ter Haar,Marthe M de Jonge,Judith R Kroep,Cor D de Kroon,Katja N Gaarenstroom,Harry Vrieling,Tjalling Bosse,Maaike P G Vreeswijk

The Journal of nutritional biochemistry 55:12-25 PubMed29331880

2018

Preventive effects of indole-3-carbinol against alcohol-induced liver injury in mice via antioxidant, anti-inflammatory, and anti-apoptotic mechanisms: Role of gut-liver-adipose tissue axis.

Applications

Unspecified application

Species

Unspecified reactive species

Youngshim Choi,Mohamed A Abdelmegeed,Byoung-Joon Song

The Journal of thoracic and cardiovascular surgery 141:1184-91 PubMed21397265

2011

Myocardial expression of heat shock protein 70i protects early postoperative right ventricular function in cyanotic tetralogy of Fallot.

Applications

Unspecified application

Species

Unspecified reactive species

Edward W K Peng,David McCaig,James C S Pollock,Kenneth MacArthur,Fiona Lyall,Mark H D Danton
View all publications

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