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Suitable for Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

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Publications

Key facts

Host species
Donkey
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
Conjugation
Alkaline Phosphatase
Applications
Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB
Clonality
Polyclonal

Reactivity data

Application
Dot
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Expected
Dilution info
1/400 - 1/2000
Notes

-

Application
ELISA
Reactivity
Expected
Dilution info
1/4000 - 1/16000
Notes

-

Application
WB
Reactivity
Expected
Dilution info
1/1000 - 1/5000
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Recommended products

Suitable for Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

Key facts

Description
Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
Host species
Donkey
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
Conjugation
Alkaline Phosphatase
Applications
Dot, IHC-Fr, IHC-P, ICC/IF, ELISA, WB
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 8
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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