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Suitable for WB, ELISA, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

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Publications

Key facts

Host species
Donkey
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Goat, Chicken, Cow, Human, Pig
Conjugation
Biotin
Applications
WB, ELISA, IHC-P, ICC/IF
Clonality
Polyclonal

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
1/10000.00000 - 1/200000.00000
Notes

(Chemiluminescent)

Application
ELISA
Reactivity
Reacts
Dilution info
1/10000.00000 - 1/200000.00000
Notes

(Primary ELISA)

Application
IHC-P
Reactivity
Reacts
Dilution info
1/250.00000 - 1/2500.00000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/100.00000 - 1/500.00000
Notes

-

Alternative names

Recommended products

Suitable for WB, ELISA, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

Key facts

Description
Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed
Host species
Donkey
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Goat, Chicken, Cow, Human, Pig
Conjugation
Biotin
Applications
WB, ELISA, IHC-P, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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