AB97083

Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed

Name: Donkey Anti Rabbit (IgG) secondary antibody preadsorbed Biotin (ab97083) | Abcam
Brand: Abcam Limited
SKU: AB97083
Price: 295 USD
Availability: InStock

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(5 Publications)

Suitable for WB, ELISA, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

IHC-P, ELISA, WB, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Chemiluminescent)" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Primary ELISA)" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Intensive care medicine experimental 12:85 PubMed39340756

2024

Association of corticosteroid therapy with reduced acute kidney injury and lower NET markers in severe COVID-19: an observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Bülow Anderberg,Joram Huckriede,Michael Hultström,Anders Larsson,Femke de Vries,Miklos Lipcsey,Gerry A F Nicolaes,Robert Frithiof

Science advances 8:eabq4736 PubMed36179027

2022

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Ma,Li Pear Wan,Yuwei Li,Chun-Hui He,Ning-Ning Song,Shiping Zhao,Huishan Wang,Yu-Qiang Ding,Bingyu Mao,Nengyin Sheng

Journal of clinical medicine 11: PubMed35743491

2022

Impaired Antibody Response Is Associated with Histone-Release, Organ Dysfunction and Mortality in Critically Ill COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Rickard Lagedal,Oskar Eriksson,Anna Sörman,Joram B Huckriede,Bjarne Kristensen,Stephanie Franzén,Anders Larsson,Anders Bergqvist,Kjell Alving,Anders Forslund,Barbro Persson,Kristina N Ekdahl,Pablo Garcia de Frutos,Bo Nilsson,Gerry A F Nicolaes,Miklos Lipcsey,Michael Hultström,Robert Frithiof

Frontiers in cellular and infection microbiology 11:694186 PubMed34568088

2021

Histone H3 Cleavage in Severe COVID-19 ICU Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Joram Huckriede,Femke de Vries,Michael Hultström,Kanin Wichapong,Chris Reutelingsperger,Miklos Lipcsey,Pablo Garcia de Frutos,Robert Frithiof,Gerry A F Nicolaes

Scientific reports 11:15701 PubMed34344929

2021

Evolution of NETosis markers and DAMPs have prognostic value in critically ill COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Joram Huckriede,Sara Bülow Anderberg,Albert Morales,Femke de Vries,Michael Hultström,Anders Bergqvist,José T Ortiz-Pérez,Jan Willem Sels,Kanin Wichapong,Miklos Lipcsey,Marcel van de Poll,Anders Larsson,Tomas Luther,Chris Reutelingsperger,Pablo Garcia de Frutos,Robert Frithiof,Gerry A F Nicolaes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise