AB96919

Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

Name: Donkey Anti-Rabbit IgG (DyLight® 488) preadsorbed secondary antibody (ab96919) | Abcam
Brand: Abcam Limited
SKU: AB96919
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 23 publications.

3 Images

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1 : 200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919) was used as a secondary antibdoy at a dilution of 1/50.

This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

Supplier Data

Western blot - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

All lanes:

rabbit anti-actin antibody at 1 µg/mL

All lanes:

HeLa whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919) at 1/1000 dilution

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Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Applications

ICC/IF, WB, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/20000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Cell structure and function 49:21-29 PubMed38797697

2024

A high-resolution phase-contrast microscopy system for label-free imaging in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Shimasaki,Yuko Okemoto-Nakamura,Kyoko Saito,Masayoshi Fukasawa,Kaoru Katoh,Kentaro Hanada

Cells 13: PubMed38607071

2024

A Novel Approach for Glioblastoma Treatment by Combining Apoptosis Inducers (TMZ, MTX, and Cytarabine) with E.V.A. (Eltanexor, Venetoclax, and A1210477) Inhibiting XPO1, Bcl-2, and Mcl-1.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhao,Madita Braun,Leonie Meyer,Katharina Otte,Hartmann Raifer,Frederik Helmprobst,Vincent Möschl,Axel Pagenstecher,Hans Urban,Michael W Ronellenfitsch,Joachim P Steinbach,Jelena Pesek,Bernhard Watzer,Wolfgang A Nockher,R Verena Taudte,Andreas Neubauer,Christopher Nimsky,Jörg W Bartsch,Tillmann Rusch

Molecular therapy : the journal of the American Society of Gene Therapy 31:2948-2961 PubMed37580905

2023

Gene augmentation therapy attenuates retinal degeneration in a knockout mouse model of Fam161a retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Matsevich,Prakadeeswari Gopalakrishnan,Ning Chang,Alexey Obolensky,Avigail Beryozkin,Manar Salameh,Corinne Kostic,Dror Sharon,Yvan Arsenijevic,Eyal Banin

Biomedicines 10: PubMed36140245

2022

Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Otte,Kai Zhao,Madita Braun,Andreas Neubauer,Hartmann Raifer,Frederik Helmprobst,Felipe Ovalle Barrera,Christopher Nimsky,Jörg W Bartsch,Tillmann Rusch

The Journal of neuroscience : the official journal of the Society for Neuroscience : PubMed35853721

2022

Deletion of the P/Q-type calcium channel from serotonergic neurons drives male aggression in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Bohne,Achim Volkmann,Martin K Schwarz,Melanie D Mark

Neuron 110:2242-2257.e6 PubMed35643078

2022

Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhong Chen,Sripriya Ravindra Kumar,Cameron D Adams,Daping Yang,Tongtong Wang,Damien A Wolfe,Cynthia M Arokiaraj,Victoria Ngo,Lillian J Campos,Jessica A Griffiths,Takako Ichiki,Sarkis K Mazmanian,Peregrine B Osborne,Janet R Keast,Cory T Miller,Andrew S Fox,Isaac M Chiu,Viviana Gradinaru

Frontiers in immunology 12:707784 PubMed34354711

2021

M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuju Li,Ming Yuan,Xue Jiao,Yufei Huang,Jing Li,Dong Li,Miaomiao Ji,Guoyun Wang

International journal of molecular sciences 22: PubMed34299212

2021

Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species.

Applications

Unspecified application

Species

Unspecified reactive species

Anberitha T Matthews,Hitesh Soni,Katherine E Robinson-Freeman,Theresa A John,Randal K Buddington,Adebowale Adebiyi

Frontiers in molecular neuroscience 14:681868 PubMed34248499

2021

Evaluation of Neuropathological Features in the SOD1-G93A Low Copy Number Transgenic Mouse Model of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Molnar-Kasza,Barbara Hinteregger,Joerg Neddens,Roland Rabl,Stefanie Flunkert,Birgit Hutter-Paier

Experimental and therapeutic medicine 20:177 PubMed33101467

2020

Antiepileptic effects of exogenous β-hydroxybutyrate on kainic acid-induced epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Si,Yingyan Wang,Jing Xu,Jiwen Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

Western blot - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (23)

Product promise