AB96892

Donkey Anti-Rabbit IgG H&L (DyLight® 550)

Name: Donkey Anti Rabbit (IgG) secondary antibody DyLight® 550 (ab96892) | Abcam
Brand: Abcam Limited
SKU: AB96892
Price: 265 USD
Availability: InStock

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(18 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 18 publications.

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Animal models and experimental medicine 7:523-532 PubMed38372410

2024

A novel mouse model of calcific aortic valve stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ningjing Qian,Yaping Wang,Wangxing Hu,Naifang Cao,Yi Qian,Jinyong Chen,Juan Fang,Dilin Xu,Haochang Hu,Shuangshuang Yang,Dao Zhou,Hanyi Dai,Dongdong Wei,Jian'an Wang,Xianbao Liu

Science advances 10:eadj1290 PubMed38354238

2024

Nutrient availability regulates the secretion and function of immune cell-derived extracellular vesicles through metabolic rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhuo Wang,Peng Lou,Yijing Xie,Shuyun Liu,Lan Li,Chengshi Wang,Dan Du,Younan Chen,Yanrong Lu,Jingqiu Cheng,Jingping Liu

Advanced materials (Deerfield Beach, Fla.) 35:e2300602 PubMed37148469

2023

Neonatal-Tissue-Derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Lou,Shuyun Liu,Yizhuo Wang,Ke Lv,Xiyue Zhou,Lan Li,Yong Zhang,Younan Chen,Jingqiu Cheng,Yanrong Lu,Jingping Liu

Cells 12: PubMed36831314

2023

Impact of Chronic Multi-Generational Exposure to an Environmentally Relevant Atrazine Concentration on Testicular Development and Function in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola D Kolaitis,Bethany J Finger,D Jo Merriner,Joseph Nguyen,Brendan J Houston,Moira K O'Bryan,Jessica M Stringer,Nadeen Zerafa,Ngoc Nguyen,Karla J Hutt,Gerard A Tarulli,Mark P Green

Cells 12: PubMed36831212

2023

An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

María José Castellanos-Montiel,Mathilde Chaineau,Anna Kristyna Franco-Flores,Ghazal Haghi,Dulce Carrillo-Valenzuela,Wolfgang E Reintsch,Carol X-Q Chen,Thomas M Durcan

Bio-protocol 12:e4360 PubMed35434191

2022

3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Kari-Anne M Frikstad,Kay O Schink,Sania Gilani,Lotte B Pedersen,Sebastian Patzke

Neurobiology of disease 164:105609 PubMed34990802

2022

Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1.

Applications

Unspecified application

Species

Unspecified reactive species

Rémi Bos,Khalil Rihan,Patrice Quintana,Lara El-Bazzal,Nathalie Bernard-Marissal,Nathalie Da Silva,Rosette Jabbour,André Mégarbané,Marc Bartoli,Frédéric Brocard,Valérie Delague

eLife 10: PubMed34259627

2021

CEP78 functions downstream of CEP350 to control biogenesis of primary cilia by negatively regulating CP110 levels.

Applications

Unspecified application

Species

Unspecified reactive species

André Brás Gonçalves,Sarah Kirstine Hasselbalch,Beinta Biskopstø Joensen,Sebastian Patzke,Pernille Martens,Signe Krogh Ohlsen,Mathieu Quinodoz,Konstantinos Nikopoulos,Reem Suleiman,Magnus Per Damsø Jeppesen,Catja Weiss,Søren Tvorup Christensen,Carlo Rivolta,Jens S Andersen,Pietro Farinelli,Lotte Bang Pedersen

Cells 9: PubMed33171886

2020

Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures.

Applications

Unspecified application

Species

Unspecified reactive species

Issan Zhang,Paula Lépine,Chanshuai Han,María Lacalle-Aurioles,Carol X-Q Chen,Rainer Haag,Thomas M Durcan,Dusica Maysinger

The EMBO journal 39:e103009 PubMed32720716

2020

Glutamine deprivation alters the origin and function of cancer cell exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Jung Fan,Benjamin Kroeger,Pauline P Marie,Esther M Bridges,John D Mason,Kristie McCormick,Christos E Zois,Helen Sheldon,Nasullah Khalid Alham,Errin Johnson,Matthew Ellis,Maria Irina Stefana,Cláudia C Mendes,Stephen Mark Wainwright,Christopher Cunningham,Freddie C Hamdy,John F Morris,Adrian L Harris,Clive Wilson,Deborah Ci Goberdhan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rabbit IgG H&L (DyLight® 550)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (18)

Product promise