AB96920

Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed

Name: Donkey Anti Rabbit (IgG) secondary antibody preadsorbed DyLight® 550 (ab96920)
Brand: Abcam Limited
SKU: AB96920
Price: 295 USD
Availability: InStock

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(6 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2423700122 PubMed40244661

2025

Intussusceptive angiogenesis-on-a-chip: Evidence for transluminal vascular bridging by endothelial delamination.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina C R Staples,Hao Yin,Frances S K Sutherland,Emma K Prescott,Dylan Tinney,Douglas W Hamilton,Daniel Goldman,Tamie L Poepping,Christopher G Ellis,J Geoffrey Pickering

Nutrients 15: PubMed36904077

2023

Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lyvianne Decourtye-Espiard,Maud Clemessy,Patricia Leneuve,Erik Mire,Tatiana Ledent,Yves Le Bouc,Laurent Kappeler

Cancers 13: PubMed33806906

2021

Persistent Inflammatory Stimulation Drives the Conversion of MSCs to Inflammatory CAFs That Promote Pro-Metastatic Characteristics in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Linor Rubinstein-Achiasaf,Dina Morein,Hagar Ben-Yaakov,Yulia Liubomirski,Tsipi Meshel,Eti Elbaz,Orly Dorot,Edward Pichinuk,Michael Gershovits,Miguel Weil,Adit Ben-Baruch

Frontiers in immunology 10:757 PubMed31031757

2019

Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yulia Liubomirski,Shalom Lerrer,Tsipi Meshel,Linor Rubinstein-Achiasaf,Dina Morein,Stefan Wiemann,Cindy Körner,Adit Ben-Baruch

Nature medicine 24:262-270 PubMed29431745

2018

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xiao,Jibo Wu,Wei-Jia Wang,Shiyang Chen,Yingxia Zheng,Xiaoqing Yu,Katrina Meeth,Mahnaz Sahraei,Alfred L M Bothwell,Lieping Chen,Marcus Bosenberg,Jianfeng Chen,Veronika Sexl,Le Sun,Lin Li,Wenwen Tang,Dianqing Wu

The European journal of neuroscience 42:3018-32 PubMed26390912

2015

Localization and expression of GABA transporters in the suprachiasmatic nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Moldavan,Olga Cravetchi,Melissa Williams,Robert P Irwin,Sue A Aicher,Charles N Allen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (6)

Product promise