AB96921

Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed

Name: Donkey Anti-Rabbit IgG (DyLight® 594) preadsorbed secondary antibody (ab96921) | Abcam
Brand: Abcam Limited
SKU: AB96921
Price: 295 USD
Availability: InStock

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(23 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 23 publications.

1 Images

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (AB96921)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (AB96921)

E14 mouse brain sample was stained with primary antibody Rabbit anti-Clim1. The secondary antibody used was ab96921 (1 : 200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 594

Excitation/Emission

Ex: 593nm, Em: 618nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:245 PubMed40549002

2025

E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Lu Zheng,Yi Wu,Qihan Ding,Shouqin Lü,Dongyuan Lü,Mian Long

Cell reports. Medicine 5:101753 PubMed39357525

2024

Leucine restriction ameliorates Fusobacterium nucleatum-driven malignant progression and radioresistance in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Songhe Guo,Shan Xing,ZhenYu Wu,Fangfang Chen,Xiaoyun Pan,Qifan Li,Wanli Liu,Ge Zhang

Biology 13: PubMed39056663

2024

Protector Role of Cx30.2 in Pancreatic β-Cell against Glucotoxicity-Induced Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Ortega-Cuellar,Ignacio González-Sánchez,Gabriela Piñón-Zárate,Marco A Cerbón,Víctor De la Rosa,Yuliana Franco-Juárez,Andrés Castell-Rodríguez,León D Islas,Cristina Coronel-Cruz

Experimental and therapeutic medicine 27:72 PubMed38234625

2024

Reducing polypyrimidine tract‑binding protein 1 fails to promote neuronal transdifferentiation on HT22 and mouse astrocyte cells under physiological conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Wei Zhang,Xin-Yu Qiao,Chong Liu,Ji-Ji Dao,Chen-Meng Qiao,Chun Cui,Yan-Qin Shen,Wei-Jiang Zhao

Neural regeneration research 19:190-195 PubMed37488866

2023

Chemokine platelet factor 4 accelerates peripheral nerve regeneration by regulating Schwann cell activation and axon elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Gu,Xiao Cheng,Di Zhang,Weiyan Wu,Yi Cao,Jianghong He

Autophagy 18:971-989 PubMed34524943

2021

Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajun Liu,Shumin Li,Xuefeng Fei,Xi Nan,Yingying Shen,Huiqing Xiu,Stephania A Cormier,Chaojie Lu,Chuqi Guo,Shibo Wang,Zhijian Cai,Pingli Wang

Journal of immunology (Baltimore, Md. : 1950) 207:296-307 PubMed34183370

2021

Neddylation Alleviates Methicillin-Resistant Infection by Inducing Macrophage Reactive Oxygen Species Production.

Applications

Unspecified application

Species

Unspecified reactive species

Huiqing Xiu,Yanmei Peng,Xiaofang Huang,Jiali Gong,Jie Yang,Jiachang Cai,Kai Zhang,Wei Cui,Yingying Shen,Jianli Wang,Shufang Zhang,Zhijian Cai,Gensheng Zhang

The Journal of physiology 598:5109-5132 PubMed32841365

2020

Tissue-specific deletion of mouse basolateral uniporter LAT4 (Slc43a2) reveals its crucial role in small intestine and kidney amino acid transport.

Applications

Unspecified application

Species

Unspecified reactive species

Anuradha Rajendran,Nadège Poncet,Lalita Oparija-Rogenmozere,Brigitte Herzog,François Verrey

PloS one 15:e0233863 PubMed32470053

2020

Phosphorylation of mouse intestinal basolateral amino acid uniporter LAT4 is controlled by food-entrained diurnal rhythm and dietary proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lalita Oparija-Rogenmozere,Anuradha Rajendran,Nadège Poncet,Simone M R Camargo,François Verrey

Fluids and barriers of the CNS 17:17 PubMed32046769

2020

Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Dolgodilina,Simone M Camargo,Eva Roth,Brigitte Herzog,Virginia Nunes,Manuel Palacín,Francois Verrey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (AB96921)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (23)

Product promise