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Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 18 publications.

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Images

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (AB96921), expandable thumbnail

Publications

Key facts

Host species
Donkey
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig
Conjugation
DyLight® 594
Applications
ICC/IF, Flow Cyt, IHC-P
Clonality
Polyclonal

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

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Alternative names

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Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 18 publications.

Key facts

Description
Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed
Host species
Donkey
Target species
Rabbit
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Goat, Horse, Chicken, Cow, Human, Pig
Conjugation
DyLight® 594
Applications
ICC/IF, Flow Cyt, IHC-P
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (ab96921), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed (ab96921)

    E14 mouse brain sample was stained with primary antibody Rabbit anti-Clim1. The secondary antibody used was ab96921 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

    This image is courtesy of an anonymous Abreview

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Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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