AB96894

Donkey Anti-Rabbit IgG H&L (DyLight® 650)

Name: Donkey Anti Rabbit (IgG) secondary antibody DyLight® 650 (ab96894) | Abcam
Brand: Abcam Limited
SKU: AB96894
Price: 265 USD
Availability: InStock

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(12 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 12 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 650 replaces DyLight® 649.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:344 PubMed41051524

2025

Cerebellar α1- adrenergic receptors mediate stress-induced dystonia in tottering mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Bohne,Mareike Josten,Lina Rambuscheck,Jana Brüggemann,Xinran Zhu,Max O Rybarski,Melanie D Mark

Frontiers in neuroanatomy 18:1400015 PubMed38817241

2024

Direct comparison of Hoxb8-driven reporter distribution in the brains of four transgenic mouse lines: towards a spinofugal projection atlas.

Applications

Unspecified application

Species

Unspecified reactive species

Bridget N Barraclough,W Terrence Stubbs,Manon Bohic,Aman Upadhyay,Victoria E Abraira,Matt S Ramer

Brain communications 6:fcae013 PubMed38638153

2024

calcium imaging shows that satellite glial cells have increased activity in painful states.

Applications

Unspecified application

Species

Unspecified reactive species

Sara E Jager,George Goodwin,Kim I Chisholm,Franziska Denk

iScience 27:109166 PubMed38433895

2024

Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to hypoactivity and synaptic abnormalities in human iPSC-derived motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Lépine,Angela Nauleau-Javaudin,Eric Deneault,Carol X-Q Chen,Narges Abdian,Anna Krystina Franco-Flores,Ghazal Haghi,María José Castellanos-Montiel,Gilles Maussion,Mathilde Chaineau,Thomas Martin Durcan

Frontiers in bioengineering and biotechnology 11:1135997 PubMed36911196

2023

Exploration and verification of COVID-19-related hub genes in liver physiological and pathological regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jihang Shi,Guangya Li,Xiandun Yuan,Yafei Wang,Ming Gong,Chonghui Li,Xinlan Ge,Shichun Lu

FEBS letters 595:2570-2592 PubMed34455585

2021

Targeting the non-ATP-binding pocket of the MAP kinase p38γ mediates a novel mechanism of cytotoxicity in cutaneous T-cell lymphoma (CTCL).

Applications

Unspecified application

Species

Unspecified reactive species

Xu Hannah Zhang,Chih-Hong Chen,Hongzhi Li,Jack Hsiang,Xiwei Wu,Weidong Hu,David Horne,Sangkil Nam,Jack Shively,Steven T Rosen

Methods (San Diego, Calif.) 203:465-477 PubMed34314827

2021

Microfabricated disk technology: Rapid scale up in midbrain organoid generation.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen-Vi Mohamed,Paula Lépine,María Lacalle-Aurioles,Julien Sirois,Meghna Mathur,Wolfgang Reintsch,Lenore K Beitel,Edward A Fon,Thomas M Durcan

PloS one 14:e0212889 PubMed30807606

2019

Role of smooth muscle cell p53 in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Wakasugi,Ippei Shimizu,Yohko Yoshida,Yuka Hayashi,Ryutaro Ikegami,Masayoshi Suda,Goro Katsuumi,Masaaki Nakao,Makoto Hoyano,Takeshi Kashimura,Kazufumi Nakamura,Hiroshi Ito,Takashi Nojiri,Tomoyoshi Soga,Tohru Minamino

Journal of tissue engineering and regenerative medicine 12:285-295 PubMed28600807

2017

Multi-layered silk film coculture system for human corneal epithelial and stromal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A Gosselin,Tess Torregrosa,Chiara E Ghezzi,Alexandra C Mendelsohn,Rachel Gomes,James L Funderburgh,David L Kaplan

The Journal of experimental medicine 214:401-422 PubMed28082357

2017

Defective ATG16L1-mediated removal of IRE1α drives Crohn's disease-like ileitis.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Tschurtschenthaler,Timon E Adolph,Jonathan W Ashcroft,Lukas Niederreiter,Richa Bharti,Svetlana Saveljeva,Joya Bhattacharyya,Magdalena B Flak,David Q Shih,Gwenny M Fuhler,Miles Parkes,Kenji Kohno,Takao Iwawaki,C Janneke van der Woude,Heather P Harding,Andrew M Smith,Maikel P Peppelenbosch,Stephan R Targan,David Ron,Philip Rosenstiel,Richard S Blumberg,Arthur Kaser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rabbit IgG H&L (DyLight® 650)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (12)

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