AB96922

Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

Name: Donkey Anti Rabbit (IgG) secondary antibody preadsorbed DyLight® 650 (ab96922)
Brand: Abcam Limited
SKU: AB96922

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(15 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 15 publications.

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Goat, Horse, Chicken, Cow, Human, Pig, Mouse

Pre-adsorbed

Yes

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments." }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Product details

DyLight® 650 replaces DyLight® 649.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 30:1406-1415 PubMed38745011

2024

Progranulin AAV gene therapy for frontotemporal dementia: translational studies and phase 1/2 trial interim results.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Sevigny,Olga Uspenskaya,Laura Dean Heckman,Li Chin Wong,Daniel A Hatch,Ambika Tewari,Rik Vandenberghe,David J Irwin,Dario Saracino,Isabelle Le Ber,Rebekah Ahmed,Jonathan D Rohrer,Adam L Boxer,Sebastian Boland,Patricia Sheehan,Alissa Brandes,Suzanne R Burstein,Benjamin M Shykind,Sitharthan Kamalakaran,Carter W Daniels,E David Litwack,Erin Mahoney,Jenny Velaga,Ilan McNamara,Patricia Sondergaard,Syed A Sajjad,Yvonne M Kobayashi,Asa Abeliovich,Franz Hefti

Cellular and molecular gastroenterology and hepatology 16:83-105 PubMed37011811

2023

DR3 Regulates Intestinal Epithelial Homeostasis and Regeneration After Intestinal Barrier Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Shimodaira,Shyam K More,Hussein Hamade,Anna Y Blackwood,Jay P Abraham,Lisa S Thomas,Jordan H Miller,Dalton T Stamps,Sofi L Castanon,Noam Jacob,Connie W Y Ha,Suzanne Devkota,David Q Shih,Stephan R Targan,Kathrin S Michelsen

Cancers 14: PubMed36551650

2022

Antagonistic Functions of Androgen Receptor and NF-κB in Prostate Cancer-Experimental and Computational Analyses.

Applications

Unspecified application

Species

Unspecified reactive species

José Basílio,Bernhard Hochreiter,Bastian Hoesel,Emira Sheshori,Marion Mussbacher,Rudolf Hanel,Johannes A Schmid

Frontiers in immunology 13:841065 PubMed35812447

2022

BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hussein Hamade,Jasmine T Stamps,Dalton T Stamps,Shyam K More,Lisa S Thomas,Anna Y Blackwood,Nawele L Lahcene,Sofi L Castanon,Brenda C Salumbides,Yosuke Shimodaira,Helen S Goodridge,Stephan R Targan,Kathrin S Michelsen

Frontiers in molecular neuroscience 13:617229 PubMed33505246

2021

Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Estibaliz Etxeberria-Rekalde,Saioa Alzola-Aldamizetxebarria,Stefanie Flunkert,Isabella Hable,Magdalena Daurer,Joerg Neddens,Birgit Hutter-Paier

Molecular cancer 20:16 PubMed33461590

2021

The inflammatory kinase IKKα phosphorylates and stabilizes c-Myc and enhances its activity.

Applications

Unspecified application

Species

Unspecified reactive species

Bernhard Moser,Bernhard Hochreiter,José Basílio,Viola Gleitsmann,Anja Panhuber,Alan Pardo-Garcia,Bastian Hoesel,Manuel Salzmann,Ulrike Resch,Mamoona Noreen,Johannes A Schmid

Frontiers in molecular neuroscience 13:136 PubMed32982685

2020

Constant Levels of Tau Phosphorylation in the Brain of htau Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joerg Neddens,Magdalena Daurer,Tina Loeffler,Saioa Alzola Aldamizetxebarria,Stefanie Flunkert,Birgit Hutter-Paier

The Journal of clinical investigation 130:5817-5832 PubMed32750042

2020

Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Ek Tan,Richard A Hopkins,Chrissie K Lim,Saumya S Jamuar,Christina Ong,Koh C Thoon,Mark Ja Koh,Eun Mong Shin,Derrick Wq Lian,Madhushanee Weerasooriya,Christopher Zw Lee,Andreas Alvin Pumomo Soetedjo,Chang Siang Lim,Veonice B Au,Edmond Chua,Hui Yin Lee,Leigh Ann Jones,Sharmy S James,Nivashini Kaliaperumal,Jeffery Kwok,Ee Shien Tan,Biju Thomas,Lynn Xue Wu,Lena Ho,Anna Marie Fairhurst,Florent Ginhoux,Adrian Kk Teo,Yong Liang Zhang,Kok Huar Ong,Weimiao Yu,Byrappa Venkatesh,Vinay Tergaonkar,Bruno Reversade,Keh Chuang Chin,Ah Moy Tan,Woei Kang Liew,John E Connolly

PloS one 15:e0235543 PubMed32645028

2020

Correlation of pyroglutamate amyloid β and ptau Ser202/Thr205 levels in Alzheimer's disease and related murine models.

Applications

Unspecified application

Species

Unspecified reactive species

Joerg Neddens,Magdalena Daurer,Stefanie Flunkert,Kerstin Beutl,Tina Loeffler,Lauren Walker,Johannes Attems,Birgit Hutter-Paier

Acta neuropathologica communications 6:52 PubMed29958544

2018

Phosphorylation of different tau sites during progression of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Joerg Neddens,Magdalena Temmel,Stefanie Flunkert,Bianca Kerschbaumer,Christina Hoeller,Tina Loeffler,Vera Niederkofler,Guenther Daum,Johannes Attems,Birgit Hutter-Paier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (15)

Product promise