Donkey Anti-Rabbit IgG H&L (HRP)

6 Images

• WB

Collaborator

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB16284)

Blocking : 5% milk

All lanes:

Western blot - Anti-Treslin antibody (<a href='/en-us/products/primary-antibodies/treslin-antibody-ab124268'>ab124268</a>) at 1/1000 dilution

Lane 1:

293 whole cell lysate treated with control siLUC RNAi at 50 µg

Lanes 2 - 3:

293 whole cell lysate treated with RNAi targeting Treslin at 50 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution

Predicted band size: 211 kDa

Observed band size: 130 kDa,250 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous abreview.

• WB

Unknown

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB16284)

All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

true

Exposure time: 150s

• ELISA

Unknown

ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (AB16284)

ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.

• WB

AbReview15966****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB16284)

All lanes:

Western blot - Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (<a href='/en-us/products/primary-antibodies/smad3-ps423-425-smad5-ps463-465-smad1-ps463-465-smad2-ps465-467-antibody-ep823y-ab52903'>ab52903</a>) at 1/1000 dilution

Lane 1:

Lysate prepared from untreated human A549 cells at 20 µg

Lane 2:

Lysate prepared from untreated human A549 cells for 30min at 20 µg

Lane 3:

Lysate prepared from TGF-ß1 cells at 10ng/ml for 30min at 20 µg

Lane 4:

Lysate prepared from TNF-a cells at 20ng/ml for 30min at 20 µg

Lane 5:

Lysate prepared from TGF-ß1 and TNF-a cells at above doses for 30min at 20 µg

Lane 6:

Blank DMEM media at 20 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

Predicted band size: 48 kDa

Observed band size: 48 kDa

true

Exposure time: 1hr

This image is a courtesy of Aaron Gardner

• WB

Unknown

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB16284)

ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

false

• WB

AbReview30978****

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (AB16284)

All lanes:

Western blot - Anti-Rad53 antibody (<a href='/en-us/products/primary-antibodies/rad53-antibody-ab104232'>ab104232</a>) at 1/1000 dilution

Lanes 1 and 5:

Alpha-factor arrested WT cells.

Lane 2:

WT cells 20' after release in S-phase.

Lane 3:

WT cells 40' after release in S-phase.

Lane 4:

WT cells 60' after release in S-phase.

Lane 6:

WT cells 20' after release in S-phase in 200mM HU.

Lane 7:

WT cells 40' after release in S-phase in 200mM HU.

Lane 8:

WT cells 60' after release in S-phase in 200mM HU.

Lanes 9 and 13:

Alpha-factor arrested rad53delta cells.

Lane 10:

rad53delta cells 20' after release in S-phase.

Lane 11:

rad53delta cells 40' after release in S-phase.

Lane 12:

rad53delta cells 60' after release in S-phase.

Lane 14:

rad53delta cells 20' after release in S-phase in 200mM HU.

Lane 15:

rad53delta cells 40' after release in S-phase in 200mM HU.

Lane 16:

rad53delta cells 60' after release in S-phase in 200mM HU.

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/2000 dilution

Predicted band size: 92 kDa

Observed band size: 92 kDa

true

Exposure time: 2min