AB7083

Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed

Name: Donkey Anti Rabbit (IgG) secondary antibody preadsorbed HRP (ab7083) | Abcam
Brand: Abcam Limited
SKU: AB7083
Price: 295 USD
Availability: InStock

Be the first to review this product! Submit a review

(25 Publications)

Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 25 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

1 Images

Supplier Data

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (AB7083)

Western blot analysis of ab7083 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Rabbit IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083) at 1/1000 dilution

false

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

IM, WB, ICC, IHC-P, IHC-Fr, Dot, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/7500 - 1/30000", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/3000", "notes":"" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Rabbit IgG

style

left-column

Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology : PubMed40804173

2025

Target sequence-conditioned design of peptide binders using masked language modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Leo Tianlai Chen,Zachary Quinn,Madeleine Dumas,Christina Peng,Lauren Hong,Moises Lopez-Gonzalez,Alexander Mestre,Rio Watson,Sophia Vincoff,Lin Zhao,Jianli Wu,Audrey Stavrand,Mayumi Schaepers-Cheu,Tian Zi Wang,Divya Srijay,Connor Monticello,Pranay Vure,Rishab Pulugurta,Sarah Pertsemlidis,Kseniia Kholina,Shrey Goel,Matthew P DeLisa,Jen-Tsan Ashley Chi,Ray Truant,Hector C Aguilar,Pranam Chatterjee

Biological & pharmaceutical bulletin 48:812-824 PubMed40500215

2025

Adipose-Derived Mesenchymal Stromal/Stem Cells Reduce Inflammatory Responses and Partially Alleviate Skin Symptoms in Imiquimod-Induced Psoriasis-Like Dermatitis Model Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohei Ogino,Mayu Tokunaga,Kenji Hayashida,Takanori Taogoshi,Tomoharu Yokooji,Hiroaki Matsuo

ACS synthetic biology 14:2354-2367 PubMed40437661

2025

Glycosylation of Structured Protein Domains in Cell-Free Reaction Environments.

Applications

Unspecified application

Species

Unspecified reactive species

Erik J Bidstrup,Kyle Hill,Chandra K Bandi,D Natasha Owitipana,Alina Chisti,Rochelle Aw,Xu Yang,Parastoo Azadi,Michael C Jewett,Lai-Xi Wang,Weston Kightlinger,Matthew P DeLisa

Nature communications 16:3555 PubMed40229275

2025

Programmable protein stabilization with language model-derived peptide guides.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Hong,Tianzheng Ye,Tian Z Wang,Divya Srijay,Howard Liu,Lin Zhao,Rio Watson,Sophia Vincoff,Tianlai Chen,Kseniia Kholina,Shrey Goel,Matthew P DeLisa,Pranam Chatterjee

Drug development research 86:e70065 PubMed39950329

2025

Exosomal ALKBH5 Alleviates Vascular Calcification by Suppressing Cell Apoptosis via m6A-Modified GSDME.

Applications

Unspecified application

Species

Unspecified reactive species

Guian Xu,Qingman Li,Lijie Zhu,Tingjie Yang,Yapan Yang,Honghui Yang

Metabolites 14: PubMed38786744

2024

Diabetes Causes Significant Alterations in Pulmonary Glucose Transporter Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Allison Campolo,Zahra Maria,Véronique A Lacombe

Oncology letters 27:8 PubMed38028180

2023

Effect of IFN‑γ encapsulated liposomes on major signal transduction pathways in the lymphocytes of patients with lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maysa Alhawamdeh,Belal Almajali,Wafa Hourani,Hamid Ali Nagi Al-Jamal,Abdullah Saleh Al-Wajeeh,Nesrin Riad Mwafi,Yousef Al-Hajaya,Hanan Kamel M Saad,Diana Anderson,Mahmoud Odeh,Ibraheam A Tarawneh

Biology of sex differences 14:8 PubMed36803626

2023

Investigation of autism-related transcription factors underlying sex differences in the effects of bisphenol A on transcriptome profiles and synaptogenesis in the offspring hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Surangrat Thongkorn,Songphon Kanlayaprasit,Kasidit Kasitipradit,Pattanachat Lertpeerapan,Pawinee Panjabud,Valerie W Hu,Depicha Jindatip,Tewarit Sarachana

Frontiers in public health 10:835914 PubMed35991009

2022

High expression of protein tyrosine phosphatase receptor S (PTPRS) is an independent prognostic marker for cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Muntinee Lertpanprom,Atit Silsirivanit,Patcharaporn Tippayawat,Tanakorn Proungvitaya,Sittiruk Roytrakul,Siriporn Proungvitaya

Journal of cellular and molecular medicine 25:3437-3448 PubMed33675119

2021

Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Jiang,Wenjing Xu,Zhenzhen Chen,Changting Cui,Xiaofang Fan,Jun Cai,Yongsheng Gong,Bin Geng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (AB7083)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (25)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise