Suitable for ICC, WB, ELISA, IHC-P. Preadsorbed to minimise non-specific binding and high background staining.
Images
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Goat, Chicken, Cow, Human, Pig
- Applications
- ICC, WB, ELISA, IHC-P
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/30000.00000 | Notes (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000) |
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/10000.00000 | Notes (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/10000) |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ICC, WB, ELISA, IHC-P. Preadsorbed to minimise non-specific binding and high background staining.
Key facts
- Description
- Donkey Anti-Rabbit IgG H&L preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Goat, Chicken, Cow, Human, Pig
- Applications
- ICC, WB, ELISA, IHC-P
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads .
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-alpha A Crystallin/CRYAA antibody ab139503 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-alpha A Crystallin/CRYAA antibody (Anti-alpha A Crystallin/CRYAA antibody ab139503) at 1 µg/mL
Lane 1: Brain (Human) Tissue Lysate - adult normal tissue at 10 µg
Lane 2: Spinal Cord (Human) Tissue Lysate - adult normal tissue at 10 µg
SecondaryAll lanes: Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa, 28 kDa
Exposure time: 30s
Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081)
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-Histone H2A.X (acetyl K5) antibody ab129217 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-Histone H2A.X (acetyl K5) antibody (Anti-Histone H2A.X (acetyl K5) antibody ab129217) at 1 µg/mL
All lanes: HeLa - Sodium butyrate treated at 10 µg
SecondaryAll lanes: Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 14 kDa
Exposure time: 8min
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