Donkey Anti-Rabbit IgG H&L preadsorbed

• WB

Unknown

Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (AB97081)

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129217 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Histone H2A.X (acetyl K5) antibody (<a href='/en-us/products/primary-antibodies/histone-h2ax-acetyl-k5-antibody-ab129217'>ab129217</a>) at 1 µg/mL

All lanes:

HeLa - Sodium butyrate treated at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 14 kDa

true

Exposure time: 8min

• WB

Unknown

Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (AB97081)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139503 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-alpha A Crystallin/CRYAA antibody (<a href='/en-us/products/primary-antibodies/alpha-a-crystallin-cryaa-antibody-ab139503'>ab139503</a>) at 1 µg/mL

Lane 1:

Brain (Human) Tissue Lysate - adult normal tissue at 10 µg

Lane 2:

Spinal Cord (Human) Tissue Lysate - adult normal tissue at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa,28 kDa

true

Exposure time: 30s