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Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 16 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670), expandable thumbnail
  • Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670), expandable thumbnail

Publications

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF

Clonality

Polyclonal

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Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/2000

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/200.00000 - 1/1000.00000

Notes

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below)
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

Alternative names

Recommended products

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 16 publications.

Alternative names

Key facts

Description

Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF

Clonality

Polyclonal

Pre-adsorbed

Yes

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175670), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175670)

    ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175670 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Rat IgG - H&L pre-adsorbed, used at 1μg/ml for 1h. DRAQ5™ (DRAQ5™ ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175670), expandable thumbnail

    Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175670)

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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