AB175670

Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed

Name: Donkey Anti-Rat IgG (Alexa Fluor® 405) preadsorbed secondary antibody (ab175670) | Abcam
Brand: Abcam Limited
SKU: AB175670
Price: 295 USD
Availability: InStock

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(23 Publications)

Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) is a preadsorbed secondary antibody with a maximum absorption wavelength of 401nm and a maximum emission wavelength of 421nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 10 publications

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175670 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Rat IgG - H&L pre-adsorbed, used at 1μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670)

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

Flow Cyt, IHC-Fr, IHC-P, ELISA, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" } } }

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Product details

This antibody reacts with the heavy and light chains of Rat IgG

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 33:101489 PubMed40496001

2025

Anti-TNF-α antisense-oligonucleotide-conjugated PLG nanoparticles protect transplanted islets.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth J Bealer,Namit Padgaonkar,Kelly Crumley,Eiji Saito,Zoe Beekman,Alexa DeKorte,Thazha P Prakash,Alexey Revenko,Lonnie D Shea

The EMBO journal 43:6199-6231 PubMed39367235

2024

Longitudinal autophagy profiling of the mammalian brain reveals sustained mitophagy throughout healthy aging.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rappe,Helena A Vihinen,Fumi Suomi,Antti J Hassinen,Homa Ehsan,Eija S Jokitalo,Thomas G McWilliams

International journal of molecular sciences 25: PubMed38891791

2024

MS785-MS27 Reactive Misfolded/Non-Native Zn-Deficient SOD1 Species Exhibit Cytotoxicity and Adopt Heterozygous Conformations in Motor Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Eiichi Tokuda,Yume Sakashita,Naoya Tokoro,Ayano Date,Yasuhiro Kosuge,Tomohiro Miyasaka

Nature communications 15:4122 PubMed38750027

2024

A non-canonical visual cortical-entorhinal pathway contributes to spatial navigation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Shao,Ligu Chen,Xiaowan Li,Miao Li,Hui Cui,Xiaoyue Li,Xinran Zhao,Yuying Shi,Qiang Sun,Kaiyue Yan,Guangfu Wang

Cell proliferation 57:e13587 PubMed38084819

2023

The characteristics of excitatory lineage differentiation and the developmental conservation in Reeler neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Huan-Huan Deng,Shi-Yuan Tong,Dan Shen,Shu-Qing Zhang,Yinghui Fu

Biomaterials 302:122316 PubMed37738741

2023

Cell-specific alterations in autophagy-lysosomal activity near the chronically implanted microelectrodes.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Chen,Camila Garcia Padilla,Kirill Kiselyov,Takashi D Y Kozai

Nature communications 14:5555 PubMed37689786

2023

PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Linnerbauer,Tobias Beyer,Lucy Nirschl,Daniel Farrenkopf,Lena Lößlein,Oliver Vandrey,Anne Peter,Thanos Tsaktanis,Hania Kebir,David Laplaud,Rupert Oellinger,Thomas Engleitner,Jorge Ivan Alvarez,Roland Rad,Thomas Korn,Bernhard Hemmer,Francisco J Quintana,Veit Rothhammer

Biomaterials 301:122210 PubMed37413842

2023

Pro-myelinating clemastine administration improves recording performance of chronically implanted microelectrodes and nearby neuronal health.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Chen,Franca Cambi,Takashi D Y Kozai

Nature immunology 24:915-924 PubMed37081147

2023

Slow integrin-dependent migration organizes networks of tissue-resident mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Kaltenbach,Paloma Martzloff,Sarah K Bambach,Nadim Aizarani,Michael Mihlan,Alina Gavrilov,Katharina M Glaser,Manuel Stecher,Roland Thünauer,Aude Thiriot,Klaus Heger,Katrin Kierdorf,Stephan Wienert,Ulrich H von Andrian,Marc Schmidt-Supprian,Claus Nerlov,Frederick Klauschen,Axel Roers,Marc Bajénoff,Dominic Grün,Tim Lämmermann

Cell reports 34:108752 PubMed33596419

2021

An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia.

Applications

Unspecified application

Species

Unspecified reactive species

Shinnosuke Suzuki,John R McCarrey,Brian P Hermann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670)

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed (AB175670)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (23)

Product promise