AB150153

Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed

Name: Donkey Anti-Rat IgG (Alexa Fluor® 488) preadsorbed secondary antibody (ab150153) | Abcam
Brand: Abcam Limited
SKU: AB150153
Price: 220 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(86 Publications)

Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) is a preadsorbed secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 60 publications

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150153)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150153)

ICC/IF image of ab6160 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody ab150153 (shown in green) was used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150153)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150153)

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-Fr, IHC-P, ICC/IF, ELISA, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Rat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (86)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:39 PubMed40824245

2025

Yes-Associated Protein (YAP) Is Associated With Disease Severity and Visual Prognosis in Epiretinal Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Seung Yang,Yong-Ha Jo,Dongwoo Kim,Suho Choi,Byung Gil Moon,Youngseop Lee,Minji Kim,Donghoon Koo,Jeongmin Kim

EBioMedicine 119:105884 PubMed40803203

2025

Tumour extracellular vesicle surface Protein-mRNA integration assay for early detection of epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Tzu Yen,Chen Zhao,Tian Gao,Jacqueline Ziqian Yang,Ning Kang,Jiahui Pu,Lei Qiu,Qixin Hu,Hyoyong Kim,Anmin Wang,Junseok Lee,Ryan Y Zhang,Na Liu,Yue Ma,You-Ren Ji,Yong Ju,Lynn L Zheng,James Lee-South,Vivian X Zuo,Audrey Qian,Aaron Kwan,Yating Zhang,Shenghua Zhang,Zhili Wang,Jing Ren,Huaichao Liu,Zihan Wang,Yang Yue,Jina Kim,Jennifer Sun,Gabriella A DiBernardo,Laura B James-Allan,Ying Chen,Weipei Zhu,Guoyun Wang,Renjun Pei,Sanaz Memarzadeh,Sungyong You,Bobbie J Rimel,Kate Lawrenson,Beth Y Karlan,Myung Shin Sim,Shaohua Lu,Jipeng Wan,Na Sun,Hsian-Rong Tseng,Yazhen Zhu

Nature cardiovascular research 4:921-937 PubMed40579458

2025

Mechanosensitive PIEZO2 channels shape coronary artery development.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Pampols-Perez,Carina Fürst,Oscar Sánchez-Carranza,Elena Cano,Jonathan Alexis Garcia-Contreras,Lisa Mais,Wenhan Luo,Sandra Raimundo,Eric L Lindberg,Martin Taube,Arnd Heuser,Anje Sporbert,Dagmar Kainmueller,Miguel O Bernabeu,Norbert Hübner,Holger Gerhardt,Gary R Lewin,Annette Hammes

Nature communications 16:4512 PubMed40374608

2025

Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3X.

Applications

Unspecified application

Species

Unspecified reactive species

Adele Mossa,Lauren Dierdorff,Jeronimo Lukin,Marta Garcia-Forn,Wei Wang,Fatemeh Mamashli,Yeaji Park,Chiara Fiorenzani,Zeynep Akpinar,Janine Kamps,Jörg Tatzelt,Zhuhao Wu,Silvia De Rubeis

Scientific reports 15:13612 PubMed40253554

2025

MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Zhao,Shuangyi Zhang,Yunjie Bai,Zhiguo Gong,Wenhui Bao,Zhuoya Yu,Bo Liu,Wei Mao,Surong Hasi

Nature communications 16:2845 PubMed40122897

2025

Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Honghui Zheng,Yilin Feng,Jiyuan Tang,Feifei Yu,Zitian Wang,Jiani Xu,Cheng Hai,Mingyue Jiang,Yifan Cheng,Zhicheng Shao,Ning Ma,Peter E Lobie,Shaohua Ma

Nature communications 16:2185 PubMed40038329

2025

E3 ubiquitin ligase RNF128 promotes Lys63-linked polyubiquitination on SRB1 in macrophages and aggravates atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yapeng Liu,Xinyu Zhang,Liwen Yu,Lei Cao,Jie Zhang,Qian Li,Xiaohong Wang,Wenqian Qi,Liangyu Cai,Ruiqing Ren,Weiwei Wang,Xiaobin Guo,Guohai Su,Bo Xi,Yun Zhang,Chengjiang Gao,Meng Zhang,Cheng Zhang

Communications biology 8:335 PubMed40021832

2025

Repetitive concussions promote microglia-mediated engulfment of presynaptic excitatory input associated with cognitive dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Chahin,Julius Mutschler,Stephanie P Dzhuleva,Clara Dieterle,Leidy Reyes Jimenez,Srijan Raj Bhattarai,Valerie Van Steenbergen,Florence M Bareyre

Renal failure 47:2459896 PubMed39972601

2025

Modified Huangqi Chifeng decoction alleviates podocyte injury on rat with experimental membranous nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Chang,Xiujie Shi,Bin Yang,Peng Li,Yifan Zhang,Qi Zhang,Yu Zhang

Nature 640:212-220 PubMed39939762

2025

Macrophages protect against sensory axon loss in peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Hakim,Aakanksha Jain,Stuart S Adamson,Veselina Petrova,Jonathan Indajang,Hyoung Woo Kim,Riki Kawaguchi,Qing Wang,Elif S Duran,Drew Nelson,Caitlin A Greene,Jenae Rasmussen,Clifford J Woolf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150153)

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150153)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (86)

Product promise