Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 61 publications.
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- Nature communications 15:14962024Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara Adem,Nuno Bastos,Carolina F Ruivo,Sara Sousa-Alves,Carolina Dias,Patrícia F Vieira,Inês A Batista,Bruno Cavadas,Dieter Saur,José C Machado,Dawen Cai,Sonia A MeloPubMed 38383468
- iScience 26:1080102023Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeshi Kameyama,Muneaki Miyata,Hajime Shiotani,Jun Adachi,Soichiro Kakuta,Yasuo Uchiyama,Kiyohito Mizutani,Yoshimi TakaiPubMed 37829206
- Neuropsychiatric disease and treatment 19:1935-19472023Oligodendrocyte Progenitor Cell Transplantation Ameliorates Preterm Infant Cerebral White Matter Injury in Rats Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaoyan Wang,Leping Zhang,Yinxiang Yang,Qian Wang,Suqing Qu,Xiaohua Wang,Zhixu He,Zuo LuanPubMed 37719062
- iScience 26:1074962023Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Liu,Lu Zhang,Zhen Liu,Jinyuan Lin,Xiaojing He,Siyi Wu,Yi Qin,Chen Zhao,Youyuan Guo,Fei LinPubMed 37636061
- iScience 26:1070512023Coordinated linear and rotational movements of endothelial cells compartmentalized by VE-cadherin drive angiogenic sprouting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuo Tonami,Tatsuya Hayashi,Yasunobu Uchijima,Masahiro Kanai,Fumitaka Yura,Jun Mada,Kei Sugahara,Yukiko Kurihara,Yuri Kominami,Toshiyuki Ushijima,Naoko Takubo,Xiaoxiao Liu,Hideto Tozawa,Yoshimitsu Kanai,Tetsuji Tokihiro,Hiroki KuriharaPubMed 37426350
- Journal of ginseng research 47:429-4392023Ginsenoside Rg3-enriched Korean Red Ginseng extract attenuates Non-Alcoholic Fatty Liver Disease by way of suppressed VCAM-1 expression in liver sinusoidal endothelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeoung-Woo Lee,Su-Min Baek,Young-Jin Lee,Tae-Un Kim,Jae-Hyuk Yim,Jun-Hyeok Son,Hee-Yeon Kim,Kyung-Ku Kang,Jong Hun Kim,Man Hee Rhee,Sang-Joon Park,Seong-Kyoon Choi,Jin-Kyu ParkPubMed 37252282
- The Journal of cell biology 222:2023Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFemmy C Stempels,Muwei Jiang,Harry M Warner,Magda-Lena Moser,Maaike H Janssens,Sjors Maassen,Iris H Nelen,Rinse de Boer,William F Jiemy,David Knight,Julian Selley,Ronan O'Cualain,Maksim V Baranov,Thomas C Q Burgers,Roberto Sansevrino,Dragomir Milovanovic,Peter Heeringa,Matthew C Jones,Rifka Vlijm,Martin Ter Beest,Geert van den BogaartPubMed 37200023
- Bio-protocol 13:e46592023Analysis of RNA Polymerase II Chromatin Binding by Flow Cytometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLilli T E Bay,Trond Stokke,Randi G Syljuåsen,Helga B LandsverkPubMed 37113330
- STAR protocols 4:1022362023Monitoring and assessment of lysosomal membrane damage in cultured cells using the high-content imager.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeisuke Tabata,Marika Saeki,Tamotsu Yoshimori,Maho HamasakiPubMed 37074905
- Development (Cambridge, England) 150:2023Cardiomyocyte ploidy is dynamic during postnatal development and varies across genetic backgrounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamantha K Swift,Alexandra L Purdy,Mary E Kolell,Kaitlyn G Andresen,Caitlin Lahue,Tyler Buddell,Kaelin A Akins,Christoph D Rau,Caitlin C O'Meara,Michaela PattersonPubMed 36912240
Key facts
- Host species
Donkey
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Human
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 61 publications.
Alternative names
Ig gamma-1 chain C region
Key facts
- Description
Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed
- Host species
Donkey
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Human
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
Polyclonal
- Pre-adsorbed
Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150153)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2μg/ml) overnight at +4°C. The secondary antibody ab150153 (shown in green) was used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150153)
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