AB150154

Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Name: Donkey Anti-Rat IgG (Alexa Fluor® 555) preadsorbed secondary antibody (ab150154) | Abcam
Brand: Abcam Limited
SKU: AB150154
Price: 195 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(82 Publications)

Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) is a preadsorbed secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 60 publications

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150154)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150154)

ICC/IF image of ab6160 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2μg/ml) overnight at +4°C. The secondary antibody ab150154 (shown in yellow) was used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150154)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150154)

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

Flow Cyt, ELISA, ICC/IF, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

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Product details

This antibody reacts with the heavy and light chains of Rat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (82)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8291 PubMed40957896

2025

Transcriptional dynamics of the oligodendrocyte lineage and its regulation by the brain erythropoietin system.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Ye,Vinicius Daguano Gastaldi,Yasmina Curto,Anne-Fleur Wildenburg,Xuan Yu,Martin Hindermann,Simone Eggert,Anja Ronnenberg,Qing Wang,Umer Javed Butt,Riki Kawaguchi,Daniel Geschwind,Wiebke Möbius,Susann Boretius,Manvendra Singh,Klaus-Armin Nave,Hannelore Ehrenreich

Cells 14: PubMed40710297

2025

Complement Modulation Mitigates Inflammation-Mediated Preterm Birth and Fetal Neural Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Eliza R McElwee,Devin Hatchell,Mohammed Alshareef,Khalil Mallah,Harriet Hall,Hannah Robinson,Ramin Eskandari,Eugene Chang,Scott Sullivan,Stephen Tomlinson

iScience 28:112935 PubMed40678520

2025

Human dorsal forebrain organoids show differentiation-state-specific protein secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Yentür,Theresa Kagermeier,Kseniia Sarieva,Mohamed A Jarboui,Katharina Becker,Simone Mayer

The Journal of clinical investigation 135: PubMed40454484

2025

Polybromo 1/vimentin axis dictates tumor grade, epithelial-mesenchymal transition, and metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Munenori Kawai,Akihisa Fukuda,Munehiro Ikeda,Kei Iimori,Kenta Mizukoshi,Kosuke Iwane,Go Yamakawa,Mayuki Omatsu,Mio Namikawa,Makoto Sono,Tomonori Masuda,Yuichi Fukunaga,Munemasa Nagao,Osamu Araki,Takaaki Yoshikawa,Satoshi Ogawa,Yukiko Hiramatsu,Motoyuki Tsuda,Takahisa Maruno,Yuki Nakanishi,Dieter Saur,Tatsuaki Tsuruyama,Toshihiko Masui,Etsuro Hatano,Hiroshi Seno

Frontiers in cellular neuroscience 19:1565939 PubMed40213497

2025

Neuropeptide Y neurons in the basolateral amygdala project to the nucleus accumbens and stimulate high-fat intake.

Applications

Unspecified application

Species

Unspecified reactive species

Shunji Yamada,Kazunori Kojima,Masaki Tanaka

Translational psychiatry 15:126 PubMed40185739

2025

Pitolisant alleviates brain network dysfunction and cognitive deficits in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zou,Linhan Yang,Jiahui Zhu,Jihua Fan,Hanrun Zheng,Xiang Liao,Zhiqi Yang,Kuan Zhang,Hongbo Jia,Arthur Konnerth,Yan-Jiang Wang,Chunqing Zhang,Yun Zhang,Sunny C Li,Xiaowei Chen

Biology of sex differences 16:19 PubMed40038827

2025

Sex differences in the microglial response to stress and chronic alcohol exposure in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexa R Soares,Vernon Garcia-Rivas,Caroline Fai,Merrilee Thomas,Xiaoying Zheng,Marina R Picciotto,Yann S Mineur

iScience 28:111756 PubMed39925433

2025

Negative regulation of lymphangiogenesis by Tenascin-C delays the resolution of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Katoh,Yoshiyuki Senga,Kento Mizutani,Kazuaki Maruyama,Daishi Yamakawa,Tadashi Yamamuro,Michiaki Hiroe,Keiichi Yamanaka,Akihiro Sudo,Naoyuki Katayama,Toshimichi Yoshida,Kyoko Imanaka-Yoshida

iScience 27:111380 PubMed39660057

2024

Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Bi,Yueguang Wu,Ning Ding,Yan Zhou,Huijuan Liu,Yongjia Weng,Qiqin Song,Li Zhang,Matthew Yibo Cheng,Heyang Cui,Weimin Zhang,Yongping Cui

Cell discovery 10:99 PubMed39349449

2024

Developing an erythrocyte‒MHC-I conjugate for cancer treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehua Liu,Xiaoqian Nie,Xingyun Yao,Huafeng Shou,Yang Yuan,Yun Ge,Xiangmin Tong,Hsiang-Ying Lee,Xiaofei Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150154)

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150154)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (82)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise