AB175475

Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed

Name: Donkey Anti-Rat IgG (Alexa Fluor® 568) preadsorbed secondary antibody (ab175475) | Abcam
Brand: Abcam Limited
SKU: AB175475
Price: 230 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(59 Publications)

Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) is a preadsorbed secondary antibody with a maximum absorption wavelength of 578nm and a maximum emission wavelength of 603nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 70 publications

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175475)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175475)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175475 Alexa Fluor® 568 donkey anti-rat IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175475)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175475)

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

Flow Cyt, IHC-Fr, ICC/IF, IHC-P, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Rat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (59)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu4270 PubMed40749060

2025

Neuronal Reg3β/macrophage TNF-α-mediated positive feedback signaling contributes to pain chronicity in a rat model of CRPS-I.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Nie,Boyu Liu,Chengyu Yin,Zishan Dong,Yushuang Pan,Peiyi Li,Qimiao Hu,Jie Wang,Yan Tai,Xiaomei Shao,Guihua Tian,Chuan Wang,Jianqiao Fang,Boyi Liu

The EMBO journal 44:1354-1378 PubMed39815005

2025

The microcephaly-associated transcriptional regulator AUTS2 cooperates with Polycomb complex PRC2 to produce upper-layer neurons in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumi Shimaoka,Kei Hori,Satoshi Miyashita,Yukiko U Inoue,Nao K N Tabe,Asami Sakamoto,Ikuko Hasegawa,Kayo Nishitani,Kunihiko Yamashiro,Saki F Egusa,Shoji Tatsumoto,Yasuhiro Go,Manabu Abe,Kenji Sakimura,Takayoshi Inoue,Takuya Imamura,Mikio Hoshino

Nature communications 15:4097 PubMed38755144

2024

FOXC1 regulates endothelial CD98 (LAT1/4F2hc) expression in retinal angiogenesis and blood-retina barrier formation.

Applications

Unspecified application

Species

Unspecified reactive species

Teena Bhakuni,Pieter R Norden,Naoto Ujiie,Can Tan,Sun Kyong Lee,Thomas Tedeschi,Yi-Wen Hsieh,Ying Wang,Ting Liu,Amani A Fawzi,Tsutomu Kume

Acta physiologica (Oxford, England) 240:e14160 PubMed38747650

2024

Striatin knock out induces a gain of function of I and impaired Ca handling in mESC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

P Benzoni,M Arici,F Giannetti,A Cospito,R Prevostini,C Volani,L Fassina,M D Rosato-Siri,A Metallo,L Gennaccaro,S Suffredini,L Foco,S Mazzetti,A Calogero,G Cappelletti,A Leibbrandt,U Elling,F Broso,J M Penninger,P P Pramstaller,C Piubelli,A Bucchi,M Baruscotti,A Rossini,M Rocchetti,A Barbuti

The Journal of clinical investigation 134: PubMed38386425

2024

Protein aggregates containing wild-type and mutant NOTCH3 are major drivers of arterial pathology in CADASIL.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Dupré,Florian Gueniot,Valérie Domenga-Denier,Virginie Dubosclard,Christelle Nilles,David Hill-Eubanks,Christelle Morgenthaler-Roth,Mark T Nelson,Céline Keime,Lydia Danglot,Anne Joutel

Cell proliferation 57:e13595 PubMed38185785

2024

Prickle1-driven basement membrane deposition of the iPSC-derived embryoid bodies is separable from the establishment of apicobasal polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Dianlei Guo,Sikai Liu,Jiao Zhang,Xinyu Gu,Lei Shi,Yingchun Su,Shujuan Xu,Rong Ju,Yanhong Wei,Chunqiao Liu

Communications biology 7:3 PubMed38168628

2024

Activation of M1 cholinergic receptors in mouse somatosensory cortex enhances information processing and detection behaviour.

Applications

Unspecified application

Species

Unspecified reactive species

Wricha Mishra,Ehsan Kheradpezhouh,Ehsan Arabzadeh

Proceedings of the National Academy of Sciences of the United States of America 120:e2306129120 PubMed37939083

2023

In vivo selection of synthetic nucleocapsids for tissue targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Olshefsky,Halli Benasutti,Meilyn Sylvestre,Gabriel L Butterfield,Gabriel J Rocklin,Christian Richardson,Derrick R Hicks,Marc J Lajoie,Kefan Song,Elizabeth Leaf,Catherine Treichel,Justin Decarreau,Sharon Ke,Gargi Kher,Lauren Carter,Jeffrey S Chamberlain,David Baker,Neil P King,Suzie H Pun

Journal of neuroinflammation 20:251 PubMed37915090

2023

The novel HS-mimetic, Tet-29, regulates immune cell trafficking across barriers of the CNS during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tessa Peck,Connor Davis,Georgia Lenihan-Geels,Maddie Griffiths,Sam Spijkers-Shaw,Olga V Zubkova,Anne Camille La Flamme

Nature protocols 18:1379-1415 PubMed37020146

2023

Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan-Ting Huang,Jesse R Poganik,Saba Parvez,Sruthi Raja,Brian Miller,Marcus J C Long,Joseph R Fetcho,Yimon Aye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175475)

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175475)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (59)

Product promise