AB150156

Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed

Name: Donkey Anti-Rat IgG (Alexa Fluor® 594) preadsorbed secondary antibody (ab150156) | Abcam
Brand: Abcam Limited
SKU: AB150156
Price: 310 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(48 Publications)

Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) is a preadsorbed secondary antibody with a maximum absorption wavelength of 590nm and a maximum emission wavelength of 617nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications

View Alternative Names

Ig gamma-1 chain C region

3 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150156 Alexa Fluor® 594 donkey anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150156 Alexa Fluor® 594 donkey anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, IHC-Fr, Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

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Product details

This antibody reacts with the heavy and light chains of Rat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (48)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:668 PubMed40708063

2025

TLR4/NF-κB-mediated M1 macrophage polarization contributes to the promotive effects of ETS2 on ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Liu,Jianshun Yu,Jie Zhang,Wei Ye,Jiaming Yao

International journal of molecular sciences 26: PubMed40076677

2025

Age-Related Differences in Lipopolysaccharide-Induced Delirium-like Behavior Implicate the Distinct Microglial Composition in the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Congli Sun,Xiaomin Kang,Xirui Jia,Yuwei Wang,Lijia Zhao,Xinyu Sun,Anaerguli Abula,Lijie Liu

iScience 28:111994 PubMed40071147

2025

IQGAP2 regulates blood-brain barrier immune dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Ketaki A Katdare,Andrew Kjar,Natasha M O'Brown,Emma H Neal,Alexander G Sorets,Alena Shostak,Wilber Romero-Fernandez,Alexander J Kwiatkowski,Kate Mlouk,Hyosung Kim,Rebecca P Cowell,Katrina R Schwensen,Cristian O Carvajal Tapia,Guste Venslovaite,Kensley B Horner,John T Wilson,Matthew S Schrag,Sean G Megason,Ethan S Lippmann

Communications biology 8:148 PubMed39885308

2025

Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factor.

Applications

Unspecified application

Species

Unspecified reactive species

Taichi Sugawara,Kayoko Sonoda,Nattapran Chompusri,Kazuhiro Noguchi,Seiji Okada,Mikio Furuse,Tomohiko Wakayama

Cell death & disease 15:824 PubMed39537618

2024

STING orchestrates microglia polarization via interaction with LC3 in autophagy after ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingqi Kong,Pengfei Xu,Nan Shen,Wenyu Li,Rui Li,Chunrong Tao,Guoping Wang,Yan Zhang,Wen Sun,Wei Hu,Xinfeng Liu

PLoS pathogens 20:e1012484 PubMed39241090

2024

Apicoplast-derived isoprenoids are essential for biosynthesis of GPI protein anchors, and consequently for egress and invasion in Plasmodium falciparum.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela S Bulloch,Long K Huynh,Kit Kennedy,Julie E Ralton,Malcolm J McConville,Stuart A Ralph

Cell reports 43:114594 PubMed39116203

2024

RTEL1 helicase counteracts RAD51-mediated homologous recombination and fork reversal to safeguard replicating genomes.

Applications

Unspecified application

Species

Unspecified reactive species

Suruchi Dixit,Tarun Nagraj,Debanjali Bhattacharya,Sneha Saxena,Satyaranjan Sahoo,Rajani Kant Chittela,Kumar Somyajit,Ganesh Nagaraju

Cell 187:3006-3023.e26 PubMed38744280

2024

Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Sacristan,Kumiko Samejima,Lorena Andrade Ruiz,Moonmoon Deb,Maaike L A Lambers,Adam Buckle,Chris A Brackley,Daniel Robertson,Tetsuya Hori,Shaun Webb,Robert Kiewisz,Tristan Bepler,Eloïse van Kwawegen,Patrik Risteski,Kruno Vukušić,Iva M Tolić,Thomas Müller-Reichert,Tatsuo Fukagawa,Nick Gilbert,Davide Marenduzzo,William C Earnshaw,Geert J P L Kops

Cancers 16: PubMed38730575

2024

Amino Terminal Acetylation of HOXB13 Regulates the DNA Damage Response in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Duy T Nguyen,Urvashi Mahajan,Duminduni Hewa Angappulige,Aashna Doshi,Nupam P Mahajan,Kiran Mahajan

Cell calcium 119:102854 PubMed38430790

2024

MLKL overexpression leads to Ca and metabolic dyshomeostasis in a neuronal cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Sathyaseelan S Deepa,Nidheesh Thadathil,Jorge Corral,Sabira Mohammed,Sophia Pham,Hadyn Rose,Michael T Kinter,Arlan Richardson,Carlos Manlio Díaz-García

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150156)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (48)

Product promise