Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) is a preadsorbed secondary antibody with a maximum absorption wavelength of 590nm and a maximum emission wavelength of 617nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications
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- Stem cell research & therapy 14:3682023New characterization and safety evaluation of human limbal stem cells used in clinical application: fidelity of mitotic process and mitotic spindle morphologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarija Zekušić,Marina Bujić Mihica,Marija Skoko,Kruno Vukušić,Patrik Risteski,Jelena Martinčić,Iva M Tolić,Krešo Bendelja,Snježana Ramić,Tamara Dolenec,Ivana Vrgoč Zimić,Dominik Puljić,Ivanka Petric Vicković,Renata Iveković,Ivanka Batarilo,Uršula Prosenc Zmrzljak,Alan Hoffmeister,Tiha VučemiloPubMed 38093301
- Experimental and therapeutic medicine 26:5682023CD138 promotes the accumulation and activation of autoreactive T cells in autoimmune MRL/lpr mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianhong Xie,Xin Liu,Ping LiPubMed 37954119
- Journal of neuroinflammation 20:2072023Transcriptomics and translatomics identify a robust inflammatory gene signature in brain endothelial cells after ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Arbaizar-Rovirosa,Mattia Gallizioli,Juan J Lozano,Julia Sidorova,Jordi Pedragosa,Sara Figuerola,Nerea Chaparro-Cabanillas,Patricia Boya,Mariona Graupera,Marc Claret,Xabier Urra,Anna M PlanasPubMed 37691115
- Nature communications 14:49602023Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYibing Han,Takeshi Tomita,Masayoshi Kato,Norihiro Ashihara,Yumiko Higuchi,Hisanori Matoba,Weiyi Wang,Hikaru Hayashi,Yuji Itoh,Satoshi Takahashi,Hiroshi Kurita,Jun Nakayama,Nobuo Okumura,Sachie HiratsukaPubMed 37620307
- International journal of biological sciences 19:4278-42902023Radioimmunotherapy Targeting B7-H3 glioma models enhanced antitumor efficacy by Reconstructing the tumor microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Zheng,Yan Wang,Fengqing Fu,Kaijie Zhang,Yanan Wang,Shandong Zhao,Qingfeng Liu,Huiwen Mu,Xueguang Zhang,Liyan MiaoPubMed 37705739
- Pigment cell & melanoma research 36:531-5412023Dynamic regulation of chromatin accessibility during melanocyte stem cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeoyeon Lee,Luye An,Paul D Soloway,Andrew C WhitePubMed 37462349
- NPJ Regenerative medicine 8:162023Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristen M Stearns-Reider,Michael R Hicks,Katherine G Hammond,Joseph C Reynolds,Alok Maity,Yerbol Z Kurmangaliyev,Jesse Chin,Adam Z Stieg,Nicholas A Geisse,Sophia Hohlbauch,Stefan Kaemmer,Lauren R Schmitt,Thanh T Pham,Ken Yamauchi,Bennett G Novitch,Roy Wollman,Kirk C Hansen,April D Pyle,Rachelle H CrosbiePubMed 36922514
- Frontiers in cellular neuroscience 16:10495622022Neurogenesis potential of oligodendrocyte precursor cells from oligospheres and injured spinal cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Zhao,Yanjing Zhu,Yilong Ren,Shuai Yin,Liqun Yu,Ruiqi Huang,Simin Song,Xiao Hu,Rongrong Zhu,Liming Cheng,Ning XiePubMed 36619671
- eLife 11:2022Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Štimac,Isabella Koprivec,Martina Manenica,Juraj Simunić,Iva M TolićPubMed 36269126
- International journal of molecular sciences 23:2022High-Fat Diet Consumption in Adolescence Induces Emotional Behavior Alterations and Hippocampal Neurogenesis Deficits Accompanied by Excessive Microglial Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuting Yao,Chenxi Yang,Conghui Wang,Hong Li,Jingyi Zhao,Xiaomin Kang,Zhuodong Liu,Lingyan Chen,Xinyu Chen,Tianshu Pu,Qinyang Li,Lijie LiuPubMed 35955450
Key facts
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Human
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200 - 1/1000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Alternative names
Ig gamma-1 chain C region
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Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) is a preadsorbed secondary antibody with a maximum absorption wavelength of 590nm and a maximum emission wavelength of 617nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 30 publications
Key facts
- Description
- Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Human
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Rat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (ab150156)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150156 Alexa Fluor® 594 donkey anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (ab150156)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150156 Alexa Fluor® 594 donkey anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (ab150156)
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