Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining.
Donkey
Rat
IgG
Heavy & Light chains
Mouse, Human
Alexa Fluor® 680
Ex: 679nm, Em: 702nm
WB
Polyclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Ig gamma-1 chain C region
Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining.
Ig gamma-1 chain C region
Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed
Donkey
Rat
IgG
Heavy & Light chains
Mouse, Human
Alexa Fluor® 680
Ex: 679nm, Em: 702nm
WB
Polyclonal
Yes
Liquid
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Affinity purification Immunogen
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
We batch test Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed, ab175777 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed, ab175777 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Terms & Conditions.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 overnight at 4°C. Antibody binding was detected using ab175777 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 5 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 2 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 1 µg
All lanes: Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed (ab175777) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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